Restriction map for pF1KB7415
 (Linear) (Six-base) MAPSORT of: KIBB7415.seq  Check: 1212  from: 1  to: 963



KIBB7415.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     963 
   Size:        102     861
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     403     435     963 
   Size:        403      32     528

  Fragments arranged by size: 

                 528     403      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     169     963 
   Size:        169     794
 
ApoI r'AATT_y

Cuts at:      0     177     246     272     293     963 
   Size:        177      69      26      21     670

  Fragments arranged by size: 

                 670     177      69      26      21
 
AvaI C'yCGr_G

Cuts at:      0     664     963 
   Size:        664     299
 
BanII G_rGCy'C

Cuts at:      0     816     963 
   Size:        816     147
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     570     963 
   Size:        570     393
 
BfrBI ATG'CAT

Cuts at:      0     388     963 
   Size:        388     575
 
Bme1580I G_kGCm'C

Cuts at:      0     514     611     963 
   Size:        514      97     352

  Fragments arranged by size: 

                 514     352      97
 
BmtI G_CTAG'C

Cuts at:      0     761     963 
   Size:        761     202
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     963 
   Size:        128     835
 
Bpu10I CC'TnA_GC

Cuts at:      0     155     963 
   Size:        155     808
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     963 
   Size:        394     569
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     110     305     963 
   Size:        110     195     658

  Fragments arranged by size: 

                 658     195     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     403     433     963 
   Size:        403      30     530

  Fragments arranged by size: 

                 530     403      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391     963 
   Size:        391     572
 
BseYI C'CCAG_C

Cuts at:      0     513     963 
   Size:        513     450
 
BsiHKAI G_wGCw'C

Cuts at:      0     816     963 
   Size:        816     147
 
BsmI GAATG_Cn'

Cuts at:      0     388     963 
   Size:        388     575
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      14     963 
   Size:         14     949
 
Bsp1286I G_dGCh'C

Cuts at:      0     514     611     816     963 
   Size:        514      97     205     147

  Fragments arranged by size: 

                 514     205     147      97
 
BspHI T'CATG_A

Cuts at:      0     108     963 
   Size:        108     855
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     633     963 
   Size:        633     330
 
BstBI TT'CG_AA

Cuts at:      0     291     963 
   Size:        291     672
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     442     644     963 
   Size:        442     202     319

  Fragments arranged by size: 

                 442     319     202
 
BtsI GCAGTG_nn'

Cuts at:      0     838     878     963 
   Size:        838      40      85

  Fragments arranged by size: 

                 838      85      40
 
DraI TTT'AAA

Cuts at:      0     542     963 
   Size:        542     421
 
EaeI y'GGCC_r

Cuts at:      0     203     255     963 
   Size:        203      52     708

  Fragments arranged by size: 

                 708     203      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     821     963 
   Size:        821     142
 
EcoICRI GAG'CTC

Cuts at:      0     814     963 
   Size:        814     149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     128     963 
   Size:        102      26     835

  Fragments arranged by size: 

                 835     102      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     151     963 
   Size:        151     812
 
EcoRI G'AATT_C

Cuts at:      0     272     963 
   Size:        272     691
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     124     156     785     817     963 
   Size:         36      32      56      32     629      32     146

  Fragments arranged by size: 

                 629     146      56      36      32      32      32
 
MscI TGG'CCA

Cuts at:      0     205     257     963 
   Size:        205      52     706

  Fragments arranged by size: 

                 706     205      52
 
MslI CAynn'nnrTG

Cuts at:      0     440     963 
   Size:        440     523
 
NheI G'CTAG_C

Cuts at:      0     757     963 
   Size:        757     206
 
NsiI A_TGCA'T

Cuts at:      0     390     963 
   Size:        390     573
 
NspI r_CATG'y

Cuts at:      0      69     963 
   Size:         69     894
 
PasI CC'CwG_GG

Cuts at:      0     644     963 
   Size:        644     319
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     403     435     963 
   Size:        403      32     528

  Fragments arranged by size: 

                 528     403      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     151     963 
   Size:        151     812
 
SacI G_AGCT'C

Cuts at:      0     816     963 
   Size:        816     147
 
SapI GCTCTTCn'nnn_

Cuts at:      0     821     963 
   Size:        821     142
 
SmlI C'TyrA_G

Cuts at:      0     373     963 
   Size:        373     590
 
SphI G_CATG'C

Cuts at:      0      69     963 
   Size:         69     894
 
SspI AAT'ATT

Cuts at:      0     873     963 
   Size:        873      90
 
StuI AGG'CCT

Cuts at:      0     371     963 
   Size:        371     592
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     265     963 
   Size:        265     698
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     AvaI    BanII     BbsI    BfrBI
 Bme1580I     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI   BstAPI    BstBI
    BstXI     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    Hin4I     MscI     MslI     NheI     NsiI     NspI     PasI
     PpiI    PpuMI     SacI     SapI     SmlI     SphI     SspI     StuI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com