Restriction map for pF1KB7408
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     AspEI       AspHI       
AvaI        AvaII       AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HgaI        Hin1I       HindIII     HpaI        
HphI        KasI        KspI        MamI        MfeI        
MluI        MroI        MspA1I      MunI        MvnI        
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        Tsp45I      Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I        395  GGTACC               G^GTACC
AseI          786  ATTAAT               AT^TAAT
AsnI          786  ATTAAT               AT^TAAT
Asp718I       395  GGTACC               G^GTACC
AspI          584  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          791  TGGCCA               TGG^CCA
BanII         388  GAGCCC               G[GA]GC[CT]^C
BsgI          757  CTGCAC               GTGCAG(16/14)
Bsp1407I      867  TGTACA               T^GTACA
BsrFI          24  GCCGGC               [GA]^CCGG[CT]
BsrGI         867  TGTACA               T^GTACA
BstXI         399  CCACGTCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          24  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I         24  GCCGGC               [GA]^CCGG[CT]
DraI          921  TTTAAA               TTT^AAA
DsaI          405  CCGTGG               C^C[GA][CT]GG
EcoRI         552  GAATTC               G^AATTC
EcoRV         820  GATATC               GAT^ATC
HapII          25  CCGG                 C^CGG
HincII        590  GTCAAC               GT[CT]^[GA]AC
HindII        590  GTCAAC               GT[CT]^[GA]AC
HpaII          25  CCGG                 C^CGG
KpnI          395  GGTACC               GGTAC^C
MluNI         791  TGGCCA               TGG^CCA
MscI          791  TGGCCA               TGG^CCA
MspI           25  CCGG                 C^CGG
NaeI           24  GCCGGC               GCC^GGC
NgoMIV         24  GCCGGC               G^CCGGC
PflFI         584  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         786  ATTAAT               AT^TAAT
SfaNI        1122  GATGC                GCATC(5/9)
SspBI         867  TGTACA               T^GTACA
SwaI          920  ATTTAAAT             ATTT^AAAT
TaqI          824  TCGA                 T^CGA
Tth111I       584  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       824  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          113  GCGG                 CCGC(-3/-1)
              335  GCGG                
BanI          191  GGTGCC               G^G[CT][GA]CC
              395  GGTACC              
BbsI          162  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BcgI          147  CGACGACTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              258  CGACGACTCTGC        
BfaI         1070  CTAG                 C^TAG
             1172  CTAG                
BglII         414  AGATCT               A^GATCT
             1032  AGATCT              
BmyI          129  GGGCAC               G[AGT]GC[ACT]^C
              388  GAGCCC              
BpmI          137  CTGGAG               CTGGAG(16/14)
              248  CTGGAG              
BpuAI         162  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BsaJI         391  CCCAGG               C^C[ACGT][ACGT]GG
              405  CCGTGG              
BsmFI         485  GGGAC                GGGAC(10/14)
              454  GTCCC               
Bsp1286I      129  GGGCAC               G[AGT]GC[ACT]^C
              388  GAGCCC              
BsrBI         111  GAGCGG               CCGCTC(-3/-3)
              333  GAGCGG              
BsrI          930  ACTGG                ACTGG(1/-1)
             1092  CCAGT               
EaeI          791  TGGCCA               [CT]^GGCC[GA]
             1049  CGGCCA              
EarI           33  CTCTTC               CTCTTC(1/4)
             1134  CTCTTC              
Ksp632I        33  CTCTTC               CTCTTC(1/4)
             1134  CTCTTC              
MaeI         1070  CTAG                 C^TAG
             1172  CTAG                
MaeIII        858  GTTAC                ^GT[ACGT]AC
             1095  GTTAC               
PleI          151  GACTC                GAGTC(4/5)
              262  GACTC               
PstI          213  CTGCAG               CTGCA^G
              324  CTGCAG              
SspI          727  AATATT               AAT^ATT
             1012  AATATT              
TfiI           16  GATTC                G^A[AT]TC
              826  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BsaI        BsiYI       BslI        BsmAI       
BssKI       BstAPI      BstNI       BstYI       CfoI        
DdeI        DpnII       EcoNI       EcoRII      Fnu4HI      
HaeII       HaeIII      HhaI        HinP1I      HinfI       
ItaI        MaeII       MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
ScrFI       SfcI        SmlI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       
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