Restriction map for pF1KB7402
 (Linear) (Six-base) MAPSORT of: KIBB7402.seq  Check: 8368  from: 1  to: 1089



KIBB7402.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

Acc65I G'GTAC_C

Cuts at:      0     465    1089 
   Size:        465     624
 
AflII C'TTAA_G

Cuts at:      0     214    1089 
   Size:        214     875
 
AlwNI CAG_nnn'CTG

Cuts at:      0     519    1089 
   Size:        519     570
 
ApaI G_GGCC'C

Cuts at:      0     727    1089 
   Size:        727     362
 
ApoI r'AATT_y

Cuts at:      0     840    1089 
   Size:        840     249
 
AvaI C'yCGr_G

Cuts at:      0      22    1089 
   Size:         22    1067
 
BanI G'GyrC_C

Cuts at:      0      99     438     465     541    1089 
   Size:         99     339      27      76     548

  Fragments arranged by size: 

                 548     339      99      76      27
 
BanII G_rGCy'C

Cuts at:      0     727     867    1089 
   Size:        727     140     222

  Fragments arranged by size: 

                 727     222     140
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     621     655    1089 
   Size:        621      34     434

  Fragments arranged by size: 

                 621     434      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     789    1089 
   Size:        789     300
 
BclI T'GATC_A

Cuts at:      0     233    1089 
   Size:        233     856
 
BlpI GC'TnA_GC

Cuts at:      0     643    1089 
   Size:        643     446
 
Bme1580I G_kGCm'C

Cuts at:      0     710     727    1089 
   Size:        710      17     362

  Fragments arranged by size: 

                 710     362      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     736     898    1089 
   Size:         57     679     162     191

  Fragments arranged by size: 

                 679     191     162      57
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    1089 
   Size:        127     962
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     163    1089 
   Size:        163     926
 
BsaAI yAC'GTr

Cuts at:      0     119    1089 
   Size:        119     970
 
BsaBI GATnn'nnATC

Cuts at:      0     682    1089 
   Size:        682     407
 
BsaWI w'CCGG_w

Cuts at:      0     527    1089 
   Size:        527     562
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     313     343     538     568     748     778    1089 
   Size:        313      30     195      30     180      30     311

  Fragments arranged by size: 

                 313     311     195     180      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     216     784    1089 
   Size:        216     568     305

  Fragments arranged by size: 

                 568     305     216
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     849    1089 
   Size:        351     498     240

  Fragments arranged by size: 

                 498     351     240
 
BsiHKAI G_wGCw'C

Cuts at:      0     354    1089 
   Size:        354     735
 
Bsp1286I G_dGCh'C

Cuts at:      0     354     710     727     867    1089 
   Size:        354     356      17     140     222

  Fragments arranged by size: 

                 356     354     222     140      17
 
BspHI T'CATG_A

Cuts at:      0     734    1089 
   Size:        734     355
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     237    1089 
   Size:        237     852
 
BsrDI GCAATG_nn'

Cuts at:      0    1067    1089 
   Size:       1067      22
 
BsrGI T'GTAC_A

Cuts at:      0     269    1089 
   Size:        269     820
 
BstYI r'GATC_y

Cuts at:      0     254     377    1089 
   Size:        254     123     712

  Fragments arranged by size: 

                 712     254     123
 
BtgI C'CryG_G

Cuts at:      0     867    1089 
   Size:        867     222
 
BtsI GCAGTG_nn'

Cuts at:      0     339    1089 
   Size:        339     750
 
DraIII CAC_nnn'GTG

Cuts at:      0     119    1081    1089 
   Size:        119     962       8

  Fragments arranged by size: 

                 962     119       8
 
EaeI y'GGCC_r

Cuts at:      0     668     848    1089 
   Size:        668     180     241

  Fragments arranged by size: 

                 668     241     180
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     496    1089 
   Size:        496     593
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     736     898    1089 
   Size:         57     679     162     191

  Fragments arranged by size: 

                 679     191     162      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     718     811    1089 
   Size:        718      93     278

  Fragments arranged by size: 

                 718     278      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     723     724    1089 
   Size:        723       1     365

  Fragments arranged by size: 

                 723     365       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     763     795    1038    1070    1089 
   Size:        143      32     588      32     243      32      19

  Fragments arranged by size: 

                 588     243     143      32      32      32      19
 
KpnI G_GTAC'C

Cuts at:      0     469    1089 
   Size:        469     620
 
MscI TGG'CCA

Cuts at:      0     670     850    1089 
   Size:        670     180     239

  Fragments arranged by size: 

                 670     239     180
 
MslI CAynn'nnrTG

Cuts at:      0      91     514    1089 
   Size:         91     423     575

  Fragments arranged by size: 

                 575     423      91
 
PfoI T'CCnGG_A

Cuts at:      0     404     713     875    1089 
   Size:        404     309     162     214

  Fragments arranged by size: 

                 404     309     214     162
 
PmlI CAC'GTG

Cuts at:      0     119    1089 
   Size:        119     970
 
PspOMI G'GGCC_C

Cuts at:      0     723    1089 
   Size:        723     366
 
SfcI C'TryA_G

Cuts at:      0     393    1089 
   Size:        393     696
 
SmlI C'TyrA_G

Cuts at:      0     142     214    1089 
   Size:        142      72     875

  Fragments arranged by size: 

                 875     142      72
 
StyI C'CwwG_G

Cuts at:      0     293     414     557     759    1089 
   Size:        293     121     143     202     330

  Fragments arranged by size: 

                 330     293     202     143     121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     986    1089 
   Size:        986     103
 
TatI w'GTAC_w

Cuts at:      0     269    1089 
   Size:        269     820
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     313     345    1089 
   Size:        313      32     744

  Fragments arranged by size: 

                 744     313      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     155    1089 
   Size:        155     934
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     300     575     969    1089 
   Size:        300     275     394     120

  Fragments arranged by size: 

                 394     300     275     120
 
XmnI GAAnn'nnTTC

Cuts at:      0     199    1089 
   Size:        199     890
 

 Enzymes that do cut:

   Acc65I    AflII    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BcgI    BciVI     BclI     BlpI Bme1580I     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BstYI     BtgI     BtsI   DraIII
     EaeI     EciI  Eco57MI    EcoNI EcoO109I    Hin4I     KpnI     MscI
     MslI     PfoI     PmlI   PspOMI     SfcI     SmlI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BfrBI     BglI
    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaHI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI     EarI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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