Restriction map for pF1KB7400
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          223  GTCTAC               GT^[AC][GT]AC
AclI          483  AACGTT               AA^CGTT
AlwNI          20  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          870  CCTAAGG              CC^T[ACGT]AGG
AspHI         631  GTGCTC               G[AT]GC[AT]^C
BalI          368  TGGCCA               TGG^CCA
BbsI          259  GAAGAC               GAAGAC(2/6)
BcgI          125  GCACCCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         784  AGATCT               A^GATCT
BmyI          631  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         259  GAAGAC               GAAGAC(2/6)
BsaAI         364  TACGTG               [CT]AC^GT[GA]
BsiHKAI       631  GTGCTC               G[AT]GC[AT]^C
BsmAI         436  GTCTC                GTCTC(1/5)
BsmI           62  GAATGC               GAATGC(1/-1)
Bsp1286I      631  GTGCTC               G[AGT]GC[ACT]^C
BspHI         857  TCATGA               T^CATGA
BsrDI         422  CATTGC               GCAATG(2/0)
Bsu36I        870  CCTAAGG              CC^T[ACGT]AGG
Cfr13I         72  GGGCC                G^G[ACGT]CC
DsaI          347  CCGTGG               C^C[GA][CT]GG
EaeI          368  TGGCCA               [CT]^GGCC[GA]
Eco81I        870  CCTAAGG              CC^T[ACGT]AGG
FauI          625  CCCGC                CCCGC(4/6)
MluNI         368  TGGCCA               TGG^CCA
MscI          368  TGGCCA               TGG^CCA
MslI          165  CACACCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         67  CAGCTG               C[AC]G^C[GT]G
NlaIV         754  GGCTCC               GG[ACGT]^[ACGT]CC
NspI          715  ACATGC               [GA]CATG^[CT]
PleI          151  GACTC                GAGTC(4/5)
PshAI         732  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      483  AACGTT               AA^CGTT
PstI          308  CTGCAG               CTGCA^G
PvuII          67  CAGCTG               CAG^CTG
RcaI          857  TCATGA               T^CATGA
Sau96I         72  GGGCC                G^G[ACGT]CC
SmlI          672  CTTGAG               C^T[CT][GA]AG
TfiI          852  GAATC                G^A[AT]TC
Tsp509I       585  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI           99  GTAC                 GT^AC
              594  GTAC                
BfaI          241  CTAG                 C^TAG
              788  CTAG                
BsiYI          75  CCTCTTCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              360  CCGCTACGTGG         
BslI           75  CCTCTTCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              360  CCGCTACGTGG         
BspMI         247  ACCTGC               ACCTGC(4/8)
              335  ACCTGC              
BsrI          184  ACTGG                ACTGG(1/-1)
              665  CCAGT               
BstF5I        636  CATCC                GGATG(2/0)
              882  GGATG               
DdeI           26  CTGAG                C^T[ACGT]AG
              871  CTAAG               
EarI           76  CTCTTC               CTCTTC(1/4)
              462  CTCTTC              
EcoT14I       545  CCTTGG               C^C[AT][AT]GG
              689  CCAAGG              
FokI          636  CATCC                GGATG(9/13)
              882  GGATG               
HinfI         151  GACTC                G^A[ACGT]TC
              852  GAATC               
Ksp632I        76  CTCTTC               CTCTTC(1/4)
              462  CTCTTC              
MaeI          241  CTAG                 C^TAG
              788  CTAG                
MaeII         365  ACGT                 A^CGT
              484  ACGT                
MseI          458  TTAA                 T^TAA
              494  TTAA                
RsaI           99  GTAC                 GT^AC
              594  GTAC                
SfaNI         251  GCATC                GCATC(5/9)
              269  GCATC               
StyI          545  CCTTGG               C^C[AT][AT]GG
              689  CCAAGG              
Tru9I         458  TTAA                 T^TAA
              494  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BseRI       BssKI       BstNI       BstYI       Cac8I       
DpnII       EcoRII      Fnu4HI      HaeIII      HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MvaI        MwoI        NdeII       NlaIII      
Sau3AI      ScrFI       SfcI        TseI        Tsp45I      
TspRI       XhoII       
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