Restriction map for pF1KB7390
 (Linear) (Six-base) MAPSORT of: KIBB7390.seq  Check: 9122  from: 1  to: 963



KIBB7390.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     118     963 
   Size:        118     845
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     510     756     963 
   Size:        213     297     246     207

  Fragments arranged by size: 

                 297     246     213     207
 
AfeI AGC'GCT

Cuts at:      0     367     963 
   Size:        367     596
 
AhdI GACnn_n'nnGTC

Cuts at:      0     455     963 
   Size:        455     508
 
AlwNI CAG_nnn'CTG

Cuts at:      0     267     765     963 
   Size:        267     498     198

  Fragments arranged by size: 

                 498     267     198
 
ApaLI G'TGCA_C

Cuts at:      0      67     963 
   Size:         67     896
 
ApoI r'AATT_y

Cuts at:      0     505     963 
   Size:        505     458
 
AvaI C'yCGr_G

Cuts at:      0     751     910     963 
   Size:        751     159      53

  Fragments arranged by size: 

                 751     159      53
 
BanI G'GyrC_C

Cuts at:      0     416     943     963 
   Size:        416     527      20

  Fragments arranged by size: 

                 527     416      20
 
BanII G_rGCy'C

Cuts at:      0      94     625     911     963 
   Size:         94     531     286      52

  Fragments arranged by size: 

                 531     286      94      52
 
BglI GCCn_nnn'nGGC

Cuts at:      0     651     963 
   Size:        651     312
 
BlpI GC'TnA_GC

Cuts at:      0      88     247     963 
   Size:         88     159     716

  Fragments arranged by size: 

                 716     159      88
 
Bme1580I G_kGCm'C

Cuts at:      0      71     946     963 
   Size:         71     875      17

  Fragments arranged by size: 

                 875      71      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     568     963 
   Size:        568     395
 
Bpu10I CC'TnA_GC

Cuts at:      0     337     963 
   Size:        337     626
 
BsaHI Gr'CG_yC

Cuts at:      0     931     963 
   Size:        931      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     443     473     963 
   Size:        443      30     490

  Fragments arranged by size: 

                 490     443      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     633     780     861     919     963 
   Size:        208     425     147      81      58      44

  Fragments arranged by size: 

                 425     208     147      81      58      44
 
BseYI C'CCAG_C

Cuts at:      0     153     260     362     626     963 
   Size:        153     107     102     264     337

  Fragments arranged by size: 

                 337     264     153     107     102
 
BsiHKAI G_wGCw'C

Cuts at:      0      64      71      94     625     731     887     963 
   Size:         64       7      23     531     106     156      76

  Fragments arranged by size: 

                 531     156     106      76      64      23       7
 
BsmI GAATG_Cn'

Cuts at:      0     170     963 
   Size:        170     793
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     922     963 
   Size:        922      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      64      71      94     625     731     887     911     946 
   Size:         64       7      23     531     106     156      24      35

Cuts at:    946     963 
   Size:         17

  Fragments arranged by size: 

                 531     156     106      64      35      24      23      17
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     118     750     963 
   Size:        118     632     213

  Fragments arranged by size: 

                 632     213     118
 
BsrFI r'CCGG_y

Cuts at:      0     407     963 
   Size:        407     556
 
BssSI C'ACGA_G

Cuts at:      0     292     963 
   Size:        292     671
 
BstBI TT'CG_AA

Cuts at:      0     162     963 
   Size:        162     801
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     873     954     963 
   Size:        873      81       9

  Fragments arranged by size: 

                 873      81       9
 
BtgI C'CryG_G

Cuts at:      0      29     938     963 
   Size:         29     909      25

  Fragments arranged by size: 

                 909      29      25
 
BtsI GCAGTG_nn'

Cuts at:      0      27     637     963 
   Size:         27     610     326

  Fragments arranged by size: 

                 610     326      27
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      33     963 
   Size:         33     930
 
EaeI y'GGCC_r

Cuts at:      0      98     350     963 
   Size:         98     252     613

  Fragments arranged by size: 

                 613     252      98
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     232     298     388     486     963 
   Size:        226       6      66      90      98     477

  Fragments arranged by size: 

                 477     226      98      90      66       6
 
EcoICRI GAG'CTC

Cuts at:      0      92     623     963 
   Size:         92     531     340

  Fragments arranged by size: 

                 531     340      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     510     756     963 
   Size:        213     297     246     207

  Fragments arranged by size: 

                 297     246     213     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0     762     869     963 
   Size:        762     107      94

  Fragments arranged by size: 

                 762     107      94
 
HaeII r_GCGC'y

Cuts at:      0     369     812     898     963 
   Size:        369     443      86      65

  Fragments arranged by size: 

                 443     369      86      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     441     473     963 
   Size:        441      32     490

  Fragments arranged by size: 

                 490     441      32
 
HincII GTy'rAC

Cuts at:      0     476     963 
   Size:        476     487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     912     963 
   Size:        912      51
 
MscI TGG'CCA

Cuts at:      0     100     963 
   Size:        100     863
 
NruI TCG'CGA

Cuts at:      0     902     963 
   Size:        902      61
 
PasI CC'CwG_GG

Cuts at:      0     873     963 
   Size:        873      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     267     963 
   Size:        267     696
 
PpuMI rG'GwC_Cy

Cuts at:      0     762     869     963 
   Size:        762     107      94

  Fragments arranged by size: 

                 762     107      94
 
PstI C_TGCA'G

Cuts at:      0     493     963 
   Size:        493     470
 
SacI G_AGCT'C

Cuts at:      0      94     625     963 
   Size:         94     531     338

  Fragments arranged by size: 

                 531     338      94
 
SexAI A'CCwGG_T

Cuts at:      0      80     865     963 
   Size:         80     785      98

  Fragments arranged by size: 

                 785      98      80
 
SfcI C'TryA_G

Cuts at:      0     489     963 
   Size:        489     474
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     420     600     963 
   Size:        376      44     180     363

  Fragments arranged by size: 

                 376     363     180      44
 
TatI w'GTAC_w

Cuts at:      0     314     963 
   Size:        314     649
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     443     475     963 
   Size:        443      32     488

  Fragments arranged by size: 

                 488     443      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     286     538     585     963 
   Size:        286     252      47     378

  Fragments arranged by size: 

                 378     286     252      47
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     249     870     963 
   Size:        249     621      93

  Fragments arranged by size: 

                 621     249      93
 
XmnI GAAnn'nnTTC

Cuts at:      0     178     963 
   Size:        178     785
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII     BglI     BlpI Bme1580I     BmrI   Bpu10I    BsaHI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrFI    BssSI    BstBI    BstXI     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MmeI
     MscI     NruI     PasI    PflMI    PpuMI     PstI     SacI    SexAI
     SfcI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     XbaI     XhoI     XmaI     ZraI
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