Restriction map for
pF1KB7388
(Circular) (Six-base) MAPSORT of: pF1KB7388.seq Check: 4227 from: 1 to: 4193
pF1KB7388 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 955 2018 2450 3197 3465 929
Size: 26 1063 432 747 268 1657
Fragments arranged by size:
1657 1063 747 432 268 26
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AleI CACnn'nnGTG
Cuts at: 997 997
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 677 952 1044 1498 3065 677
Size: 275 92 454 1567 1805
Fragments arranged by size:
1805 1567 454 275 92
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 147 183 289 1197 3546 3802 147
Size: 36 106 908 2349 256 538
Fragments arranged by size:
2349 908 538 256 106 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1213 1213
Size: 4193
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 1209 1623 1871 1906 1209
Size: 414 248 35 3496
Fragments arranged by size:
3496 414 248 35
BanII G_rGCy'C
Cuts at: 1207 2237 1207
Size: 1030 3163
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 798 1713 798
Size: 915 3278
BfrBI ATG'CAT
Cuts at: 896 896
Size: 4193
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 1818 1911 2967 1818
Size: 93 1056 3044
Fragments arranged by size:
3044 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BmtI G_CTAG'C
Cuts at: 604 604
Size: 4193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 406
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 427 1624 1872 2574 4070 427
Size: 1197 248 702 1496 550
Fragments arranged by size:
1496 1197 702 550 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 300 300
Size: 4193
BseYI C'CCAG_C
Cuts at: 1618 2376 2953 3449 1618
Size: 758 577 496 2362
Fragments arranged by size:
2362 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 4193
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
BsmI GAATG_Cn'
Cuts at: 707 707
Size: 4193
BsmBI CGTCTCn'nnnn_
Cuts at: 424 424
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 1207 1818 1911 1985 2175 2237 2967 1207
Size: 611 93 74 190 62 730 2433
Fragments arranged by size:
2433 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 605 1243 1457 1759 2140 392
Size: 213 638 214 302 381 2445
Fragments arranged by size:
2445 638 381 302 214 213
BsrBI CCG'CTC
Cuts at: 50 336 407 2485 2539 4023 4188 50
Size: 286 71 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 286 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstEII G'GTnAC_C
Cuts at: 999 999
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 258 258
Size: 4193
BstYI r'GATC_y
Cuts at: 1 1218 1685 1708 2043 2289 3301 3387 3399
Size: 1217 467 23 335 246 1012 86 12
Cuts at: 3399 3745 1
Size: 346 449
Fragments arranged by size:
1217 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 320 320
Size: 4193
BtgI C'CryG_G
Cuts at: 107 608 107
Size: 501 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 809 2143 2324 2487 809
Size: 1334 181 163 2515
Fragments arranged by size:
2515 1334 181 163
BtsI GCAGTG_nn'
Cuts at: 1436 1436
Size: 4193
DraI TTT'AAA
Cuts at: 146 1192 146
Size: 1046 3147
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 398 611 629 1778 1952 2343 2370 3715 398
Size: 213 18 1149 174 391 27 1345 876
Fragments arranged by size:
1345 1149 876 391 213 174 27 18
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 929 955 2018 2450 3197 3465 406
Size: 523 26 1063 432 747 268 1134
Fragments arranged by size:
1134 1063 747 523 432 268 26
EcoO109I rG'GnC_Cy
Cuts at: 1332 1332
Size: 4193
EcoRI G'AATT_C
Cuts at: 289 1197 289
Size: 908 3285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 2212 354
Size: 1858 2335
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 1627 1875 2897 3876 1627
Size: 248 1022 979 1944
Fragments arranged by size:
1944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 665 697 1167 1199 2035 2065 2067 2097 665
Size: 32 470 32 836 30 2 30 2761
Fragments arranged by size:
2761 836 470 32 32 30 30 2
HincII GTy'rAC
Cuts at: 465 1232 465
Size: 767 3426
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 189 1073 2864 3048 189
Size: 884 1791 184 1334
Fragments arranged by size:
1791 1334 884 184
MscI TGG'CCA
Cuts at: 400 613 631 1954 400
Size: 213 18 1323 2639
Fragments arranged by size:
2639 1323 213 18
MslI CAynn'nnrTG
Cuts at: 256 661 853 997 2182 2309 256
Size: 405 192 144 1185 127 2140
Fragments arranged by size:
2140 1185 405 192 144 127
MspA1I CmG'CkG
Cuts at: 949 1304 1618 1978 2991 3236 949
Size: 355 314 360 1013 245 1906
Fragments arranged by size:
1906 1013 360 355 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 107 608 107
Size: 501 3692
NdeI CA'TA_TG
Cuts at: 634 634
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NheI G'CTAG_C
Cuts at: 600 600
Size: 4193
NsiI A_TGCA'T
Cuts at: 898 898
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PshAI GACnn'nnGTC
Cuts at: 416 416
Size: 4193
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 949 1618 1978 949
Size: 669 360 3164
Fragments arranged by size:
3164 669 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 1122 1236 1921 2914 3105 33
Size: 1089 114 685 993 191 1121
Fragments arranged by size:
1121 1089 993 685 191 114
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1348 2263 2755 3017 1348
Size: 915 492 262 2524
Fragments arranged by size:
2524 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 300 608 1327 1681 107
Size: 193 308 719 354 2619
Fragments arranged by size:
2619 719 354 308 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 434 560 3708 434
Size: 126 3148 919
Fragments arranged by size:
3148 919 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
Bpu10I BsaXI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI