Restriction map for pF1KB7384
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsgI        BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     FauI        
FbaI        FseI        FspI        HaeII       Hin1I       
KasI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII         718  CGCG                 CG^CG
AccIII         89  TCCGGA               T^CCGGA
AflIII        203  ACACGT               A^C[GA][CT]GT
Asp700I       627  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglII        1319  AGATCT               A^GATCT
BpmI         1211  CTCCAG               CTGGAG(16/14)
BsaAI         204  CACGTA               [CT]AC^GT[GA]
BsaWI          89  TCCGGA               [AT]^CCGG[AT]
BseAI          89  TCCGGA               T^CCGGA
BsmAI        1047  GTCTC                GTCTC(1/5)
BspEI          89  TCCGGA               T^CCGGA
BstUI         718  CGCG                 CG^CG
BstXI         636  CCATTACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         410  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        111  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          275  CCATGG               C^C[GA][CT]GG
EcoNI        1108  CCTAGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      410  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         467  GAATTC               G^AATTC
EcoRV         729  GATATC               GAT^ATC
EcoT14I       275  CCATGG               C^C[AT][AT]GG
EcoT22I       580  ATGCAT               ATGCA^T
HindIII       657  AAGCTT               A^AGCTT
HpaI          961  GTTAAC               GTT^AAC
MroI           89  TCCGGA               T^CCGGA
MvnI          718  CGCG                 CG^CG
NcoI          275  CCATGG               C^CATGG
NotI           55  GCGGCCGC             GC^GGCCGC
NsiI          580  ATGCAT               ATGCA^T
PflMI         967  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         410  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         811  GACTTTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          302  CTGCAG               CTGCA^G
SacI          613  GAGCTC               GAGCT^C
SapI          232  GAAGAGC              GCTCTTC(1/4)
StyI          275  CCATGG               C^C[AT][AT]GG
Van91I        967  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          627  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        646  GGTACC               G^GTACC
             1162  GGTACC              
AcsI          467  GAATTC               [GA]^AATT[CT]
             1382  AAATTC              
AlwI          291  GATCC                GGATC(4/5)
              988  GATCC               
ApoI          467  GAATTC               [GA]^AATT[CT]
             1382  AAATTC              
Asp718I       646  GGTACC               G^GTACC
             1162  GGTACC              
AvaII         411  GGTCC                G^G[AT]CC
              442  GGACC               
BalI          278  TGGCCA               TGG^CCA
             1284  TGGCCA              
BanII         235  GAGCCC               G[GA]GC[CT]^C
              613  GAGCTC              
BbsI          883  GAAGAC               GAAGAC(2/6)
             1005  GAAGAC              
BpuAI         883  GAAGAC               GAAGAC(2/6)
             1005  GAAGAC              
BsiEI          56  CGGCCG               CG[GA][CT]^CG
              159  CGGCCG              
BstF5I       1075  CATCC                GGATG(2/0)
              539  GGATG               
BstNI         165  CCAGG                CC^[AT]GG
              354  CCTGG               
BstYI         290  AGATCC               [GA]^GATC[CT]
             1319  AGATCT              
CfoI          719  GCGC                 GCG^C
             1014  GCGC                
EagI           56  CGGCCG               C^GGCCG
              159  CGGCCG              
EclXI          56  CGGCCG               C^GGCCG
              159  CGGCCG              
Eco52I         56  CGGCCG               C^GGCCG
              159  CGGCCG              
EcoRII        165  CCAGG                ^CC[AT]GG
              354  CCTGG               
FokI         1075  CATCC                GGATG(9/13)
              539  GGATG               
HgaI          101  GCGTC                GACGC(5/10)
              119  GCGTC               
HhaI          719  GCGC                 GCG^C
             1014  GCGC                
HinP1I        719  GCGC                 G^CGC
             1014  GCGC                
HphI          229  GGTGA                GGTGA(8/7)
             1207  TCACC               
KpnI          646  GGTACC               GGTAC^C
             1162  GGTACC              
MaeII         143  ACGT                 A^CGT
              205  ACGT                
MflI          290  AGATCC               [GA]^GATC[CT]
             1319  AGATCT              
MluNI         278  TGGCCA               TGG^CCA
             1284  TGGCCA              
MscI          278  TGGCCA               TGG^CCA
             1284  TGGCCA              
MslI          637  CATTACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1231  CACGACTGTG          
MspA1I        299  CAGCTG               C[AC]G^C[GT]G
             1361  CAGCTG              
MvaI          165  CCAGG                CC^[AT]GG
              354  CCTGG               
PvuII         299  CAGCTG               CAG^CTG
             1361  CAGCTG              
TaqI          333  TCGA                 T^CGA
              741  TCGA                
Tsp45I        250  GTCAC                ^GT[GC]AC
              532  GTGAC               
TspRI         185  CACTG                CA[GC]TG[ACGT][ACGT]^
              252  CACTG               
TthHB8I       333  TCGA                 T^CGA
              741  TCGA                
XhoII         290  AGATCC               [GA]^GATC[CT]
             1319  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BanI        
BbvI        BcnI        BfaI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EarI        Fnu4HI      
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TfiI        Tru9I       TseI        
Tsp509I     
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