Restriction map for pF1KB7384
 (Linear) (Six-base) MAPSORT of: KIBB7384.seq  Check: 8730  from: 1  to: 1392



KIBB7384.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

Acc65I G'GTAC_C

Cuts at:      0     646    1162    1392 
   Size:        646     516     230

  Fragments arranged by size: 

                 646     516     230
 
AflIII A'CryG_T

Cuts at:      0     203    1392 
   Size:        203    1189
 
AleI CACnn'nnGTG

Cuts at:      0    1235    1392 
   Size:       1235     157
 
ApoI r'AATT_y

Cuts at:      0     467    1382    1392 
   Size:        467     915      10

  Fragments arranged by size: 

                 915     467      10
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     630     638     663     671    1392 
   Size:        630       8      25       8     721

  Fragments arranged by size: 

                 721     630      25       8       8
 
BanI G'GyrC_C

Cuts at:      0     126     646    1162    1392 
   Size:        126     520     516     230

  Fragments arranged by size: 

                 520     516     230     126
 
BanII G_rGCy'C

Cuts at:      0     239     617    1392 
   Size:        239     378     775

  Fragments arranged by size: 

                 775     378     239
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     890    1012    1392 
   Size:        890     122     380

  Fragments arranged by size: 

                 890     380     122
 
BfrBI ATG'CAT

Cuts at:      0     582    1392 
   Size:        582     810
 
BglII A'GATC_T

Cuts at:      0    1319    1392 
   Size:       1319      73
 
Bme1580I G_kGCm'C

Cuts at:      0     131    1392 
   Size:        131    1261
 
BmgBI CAC'GTC

Cuts at:      0     144    1392 
   Size:        144    1248
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     310     312     330    1392 
   Size:        310       2      18    1062

  Fragments arranged by size: 

                1062     310      18       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1196    1392 
   Size:       1196     196
 
BsaAI yAC'GTr

Cuts at:      0     206    1392 
   Size:        206    1186
 
BsaWI w'CCGG_w

Cuts at:      0      89    1392 
   Size:         89    1303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     684    1028    1167    1392 
   Size:        684     344     139     225

  Fragments arranged by size: 

                 684     344     225     139
 
BseYI C'CCAG_C

Cuts at:      0     130    1392 
   Size:        130    1262
 
BsiEI CG_ry'CG

Cuts at:      0      59     162    1392 
   Size:         59     103    1230

  Fragments arranged by size: 

                1230     103      59
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     311     564     617    1392 
   Size:        143     168     253      53     775

  Fragments arranged by size: 

                 775     253     168     143      53
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     143     239     311     564     617    1392 
   Size:        131      12      96      72     253      53     775

  Fragments arranged by size: 

                 775     253     131      96      72      53      12
 
BspEI T'CCGG_A

Cuts at:      0      89    1392 
   Size:         89    1303
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     643    1392 
   Size:        643     749
 
BstYI r'GATC_y

Cuts at:      0     290    1319    1392 
   Size:        290    1029      73

  Fragments arranged by size: 

                1029     290      73
 
BtgI C'CryG_G

Cuts at:      0     275    1392 
   Size:        275    1117
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      25    1392 
   Size:         25    1367
 
DraIII CAC_nnn'GTG

Cuts at:      0     116    1392 
   Size:        116    1276
 
EaeI y'GGCC_r

Cuts at:      0      56     159     278    1284    1392 
   Size:         56     103     119    1006     108

  Fragments arranged by size: 

                1006     119     108     103      56
 
EagI C'GGCC_G

Cuts at:      0      56     159    1392 
   Size:         56     103    1233

  Fragments arranged by size: 

                1233     103      56
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     617    1185    1300    1392 
   Size:        227     390     568     115      92

  Fragments arranged by size: 

                 568     390     227     115      92
 
EcoICRI GAG'CTC

Cuts at:      0     615    1392 
   Size:        615     777
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1196    1392 
   Size:       1196     196
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1112    1392 
   Size:       1112     280
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411    1392 
   Size:        411     981
 
EcoRI G'AATT_C

Cuts at:      0     467    1392 
   Size:        467     925
 
EcoRV GAT'ATC

Cuts at:      0     731    1392 
   Size:        731     661
 
HincII GTy'rAC

Cuts at:      0     105     555     897     963    1392 
   Size:        105     450     342      66     429

  Fragments arranged by size: 

                 450     429     342     105      66
 
HindIII A'AGCT_T

Cuts at:      0     657    1392 
   Size:        657     735
 
HpaI GTT'AAC

Cuts at:      0     963    1392 
   Size:        963     429
 
KpnI G_GTAC'C

Cuts at:      0     650    1166    1392 
   Size:        650     516     226

  Fragments arranged by size: 

                 650     516     226
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     844    1392 
   Size:        420     424     548

  Fragments arranged by size: 

                 548     424     420
 
MscI TGG'CCA

Cuts at:      0     280    1286    1392 
   Size:        280    1006     106

  Fragments arranged by size: 

                1006     280     106
 
MslI CAynn'nnrTG

Cuts at:      0     641    1235    1392 
   Size:        641     594     157

  Fragments arranged by size: 

                 641     594     157
 
MspA1I CmG'CkG

Cuts at:      0     301    1363    1392 
   Size:        301    1062      29

  Fragments arranged by size: 

                1062     301      29
 
NcoI C'CATG_G

Cuts at:      0     275    1392 
   Size:        275    1117
 
NotI GC'GGCC_GC

Cuts at:      0      56    1392 
   Size:         56    1336
 
NsiI A_TGCA'T

Cuts at:      0     584    1392 
   Size:        584     808
 
PasI CC'CwG_GG

Cuts at:      0     354    1392 
   Size:        354    1038
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     973    1392 
   Size:        973     419
 
PpuMI rG'GwC_Cy

Cuts at:      0     411    1392 
   Size:        411     981
 
PshAI GACnn'nnGTC

Cuts at:      0     815    1392 
   Size:        815     577
 
PstI C_TGCA'G

Cuts at:      0     306    1392 
   Size:        306    1086
 
PvuII CAG'CTG

Cuts at:      0     301    1363    1392 
   Size:        301    1062      29

  Fragments arranged by size: 

                1062     301      29
 
SacI G_AGCT'C

Cuts at:      0     617    1392 
   Size:        617     775
 
SanDI GG'GwC_CC

Cuts at:      0     411    1392 
   Size:        411     981
 
SapI GCTCTTCn'nnn_

Cuts at:      0     227    1392 
   Size:        227    1165
 
SfcI C'TryA_G

Cuts at:      0     302     776     872    1392 
   Size:        302     474      96     520

  Fragments arranged by size: 

                 520     474     302      96
 
StyI C'CwwG_G

Cuts at:      0     275    1392 
   Size:        275    1117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     572    1392 
   Size:        572     820
 
TatI w'GTAC_w

Cuts at:      0    1111    1392 
   Size:       1111     281
 
XmnI GAAnn'nnTTC

Cuts at:      0     631    1392 
   Size:        631     761
 

 Enzymes that do cut:

   Acc65I   AflIII     AleI     ApoI     BaeI     BanI    BanII     BbsI
    BfrBI    BglII Bme1580I    BmgBI     BplI     BpmI    BsaAI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BstXI    BstYI
     BtgI    BtgZI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV   HincII  HindIII     HpaI     KpnI
     MmeI     MscI     MslI   MspA1I     NcoI     NotI     NsiI     PasI
    PflMI    PpuMI    PshAI     PstI    PvuII     SacI    SanDI     SapI
     SfcI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
     DrdI     EciI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NruI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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