Restriction map for pF1KB7382
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AflII       AflIII      AgeI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanII       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       BstAPI      BstUI       BstXI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

AcsI           31  GAATTT               [GA]^AATT[CT]
AcyI          695  GGCGCC               G[GA]^CG[CT]C
AfaI          455  GTAC                 GT^AC
AhdI          486  GACTATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI           31  GAATTT               [GA]^AATT[CT]
Asp700I       846  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         486  GACTATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          695  GGCGCC               G^G[CT][GA]CC
BbeI          695  GGCGCC               GGCGC^C
BbvI          897  GCAGC                GCAGC(8/12)
BclI          344  TGATCA               T^GATCA
BcnI           60  CCGGG                CC^[GC]GG
BfaI          469  CTAG                 C^TAG
BglII         332  AGATCT               A^GATCT
BsaAI         367  TACGTG               [CT]AC^GT[GA]
BsaHI         695  GGCGCC               G[GA]^CG[CT]C
BsaJI         638  CCGTGG               C^C[ACGT][ACGT]GG
BseRI         874  GAGGAG               GAGGAG(10/8)
BsmFI          45  GGGAC                GGGAC(10/14)
BsmI           88  GCATTC               GAATGC(1/-1)
BspMI         715  ACCTGC               ACCTGC(4/8)
BsrDI         138  CATTGC               GCAATG(2/0)
Bst1107I      422  GTATAC               GTA^TAC
BstBI         674  TTCGAA               TT^CGAA
BstF5I        660  CATCC                GGATG(2/0)
BstNI         663  CCTGG                CC^[AT]GG
BstYI         332  AGATCT               [GA]^GATC[CT]
BstZ17I       422  GTATAC               GTA^TAC
CfoI          696  GCGC                 GCG^C
DsaI          638  CCGTGG               C^C[GA][CT]GG
Eam1105I      486  GACTATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        663  CCTGG                ^CC[AT]GG
FbaI          344  TGATCA               T^GATCA
Fnu4HI        897  GCAGC                GC^[ACGT]GC
FokI          660  CATCC                GGATG(9/13)
HaeII         695  GGCGCC               [GA]GCGC^[CT]
HapII          60  CCGG                 C^CGG
HhaI          696  GCGC                 GCG^C
Hin1I         695  GGCGCC               G[GA]^CG[CT]C
HinP1I        696  GCGC                 G^CGC
HpaII          60  CCGG                 C^CGG
ItaI          897  GCAGC                GC^[ACGT]GC
KasI          695  GGCGCC               G^GCGCC
MaeI          469  CTAG                 C^TAG
MaeII         368  ACGT                 A^CGT
MflI          332  AGATCT               [GA]^GATC[CT]
MslI           99  CATTTATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        686  CAGCTG               C[AC]G^C[GT]G
MspI           60  CCGG                 C^CGG
MvaI          663  CCTGG                CC^[AT]GG
NarI          695  GGCGCC               GG^CGCC
NciI           60  CCGGG                CC^[GC]GG
NspV          674  TTCGAA               TT^CGAA
PflMI         726  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         686  CAGCTG               CAG^CTG
RsaI          455  GTAC                 GT^AC
SapI          300  GCTCTTC              GCTCTTC(1/4)
SfaNI         290  GCATC                GCATC(5/9)
SfoI          695  GGCGCC               GGC^GCC
SfuI          674  TTCGAA               TT^CGAA
SmlI          864  CTTGAG               C^T[CT][GA]AG
TaqI          675  TCGA                 T^CGA
TfiI          846  GAATC                G^A[AT]TC
TseI          897  GCAGC                G^C[AT]GC
Tsp509I        32  AATT                 ^AATT
TthHB8I       675  TCGA                 T^CGA
Van91I        726  CCACTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          797  CCATTGACAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         332  AGATCT               [GA]^GATC[CT]
XmnI          846  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BbsI           37  GTCTTC               GAAGAC(2/6)
              817  GTCTTC              
BpuAI          37  GTCTTC               GAAGAC(2/6)
              817  GTCTTC              
BsgI          378  CTGCAC               GTGCAG(16/14)
              564  CTGCAC              
BsmAI         909  GAGAC                GTCTC(1/5)
              161  GTCTC               
BssKI          60  CCGGG                ^CC[ACGT]GG
              663  CCTGG               
BstEII        642  GGTCACC              G^GT[ACGT]ACC
              831  GGTCACC             
BstPI         642  GGTCACC              G^GT[ACGT]ACC
              831  GGTCACC             
Cac8I         558  GCTGGC               GC[ACGT]^[ACGT]GC
              562  GCCTGC              
EaeI          635  TGGCCG               [CT]^GGCC[GA]
              778  CGGCCA              
EcoO65I       642  GGTCACC              G^GT[ACGT]ACC
              831  GGTCACC             
HphI          644  TCACC                GGTGA(8/7)
              833  TCACC               
PleI           47  GACTC                GAGTC(4/5)
              416  GAGTC               
ScrFI          60  CCGGG                CC^[ACGT]GG
              663  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AccI        AluI        BsiYI       BslI        Cfr13I      
DdeI        DpnII       EarI        HaeIII      HinfI       
Ksp632I     MaeIII      MboI        MboII       MnlI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      Tsp45I      TspRI       
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