Restriction map for pF1KB7382
 (Linear) (Six-base) MAPSORT of: KIBB7382.seq  Check: 8056  from: 1  to: 939



KIBB7382.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     423     495     712     939 
   Size:        423      72     217     227

  Fragments arranged by size: 

                 423     227     217      72
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710     939 
   Size:        710     229
 
AhdI GACnn_n'nnGTC

Cuts at:      0     491     939 
   Size:        491     448
 
ApoI r'AATT_y

Cuts at:      0      31     939 
   Size:         31     908
 
BanI G'GyrC_C

Cuts at:      0     695     939 
   Size:        695     244
 
BbeI G_GCGC'C

Cuts at:      0     699     939 
   Size:        699     240
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     810     939 
   Size:         30     780     129

  Fragments arranged by size: 

                 780     129      30
 
BclI T'GATC_A

Cuts at:      0     344     939 
   Size:        344     595
 
BglII A'GATC_T

Cuts at:      0     332     939 
   Size:        332     607
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     901     939 
   Size:        187     714      38

  Fragments arranged by size: 

                 714     187      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     885     939 
   Size:        885      54
 
BsaAI yAC'GTr

Cuts at:      0     369     939 
   Size:        369     570
 
BsaHI Gr'CG_yC

Cuts at:      0     696     939 
   Size:        696     243
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     889     939 
   Size:        889      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     549     939 
   Size:        363     186     390

  Fragments arranged by size: 

                 390     363     186
 
BsmI GAATG_Cn'

Cuts at:      0      88     939 
   Size:         88     851
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724     939 
   Size:        724     215
 
BsrDI GCAATG_nn'

Cuts at:      0     137     939 
   Size:        137     802
 
BstBI TT'CG_AA

Cuts at:      0     675     939 
   Size:        675     264
 
BstEII G'GTnAC_C

Cuts at:      0     642     831     939 
   Size:        642     189     108

  Fragments arranged by size: 

                 642     189     108
 
BstYI r'GATC_y

Cuts at:      0     332     939 
   Size:        332     607
 
BstZ17I GTA'TAC

Cuts at:      0     424     939 
   Size:        424     515
 
BtgI C'CryG_G

Cuts at:      0     638     939 
   Size:        638     301
 
EaeI y'GGCC_r

Cuts at:      0     635     778     939 
   Size:        635     143     161

  Fragments arranged by size: 

                 635     161     143
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     677     751     939 
   Size:        307     370      74     188

  Fragments arranged by size: 

                 370     307     188      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710     939 
   Size:        710     229
 
HaeII r_GCGC'y

Cuts at:      0     699     939 
   Size:        699     240
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     477     509     939 
   Size:        477      32     430

  Fragments arranged by size: 

                 477     430      32
 
KasI G'GCGC_C

Cuts at:      0     695     939 
   Size:        695     244
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     939 
   Size:        224     715
 
MslI CAynn'nnrTG

Cuts at:      0     103     939 
   Size:        103     836
 
MspA1I CmG'CkG

Cuts at:      0     688     939 
   Size:        688     251
 
NarI GG'CG_CC

Cuts at:      0     696     939 
   Size:        696     243
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     732     939 
   Size:        732     207
 
PvuII CAG'CTG

Cuts at:      0     688     939 
   Size:        688     251
 
SapI GCTCTTCn'nnn_

Cuts at:      0     307     939 
   Size:        307     632
 
SfoI GGC'GCC

Cuts at:      0     697     939 
   Size:        697     242
 
SmlI C'TyrA_G

Cuts at:      0     864     939 
   Size:        864      75
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     804     939 
   Size:        804     135
 
XmnI GAAnn'nnTTC

Cuts at:      0     850     939 
   Size:        850      89
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     ApoI     BanI     BbeI     BbsI     BclI
    BglII   Bpu10I    BpuEI    BsaAI    BsaHI    BseRI     BsgI     BsmI
    BspMI    BsrDI    BstBI   BstEII    BstYI  BstZ17I     BtgI     EaeI
     EarI  Eco57MI    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I
     NarI    PflMI    PvuII     SapI     SfoI     SmlI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI     BsaI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstXI   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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