Restriction map for pF1KB7373
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          617  AAATTC               [GA]^AATT[CT]
AfaI          538  GTAC                 GT^AC
AlwNI         557  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          617  AAATTC               [GA]^AATT[CT]
AvaII         343  GGACC                G^G[AT]CC
BalI           93  TGGCCA               TGG^CCA
BanII         421  GAGCCC               G[GA]GC[CT]^C
BbsI           77  GAAGAC               GAAGAC(2/6)
BcnI          298  CCGGG                CC^[GC]GG
BmyI          421  GAGCCC               G[AGT]GC[ACT]^C
BpmI           72  CTCCAG               CTGGAG(16/14)
BpuAI          77  GAAGAC               GAAGAC(2/6)
BseRI         528  CTCCTC               GAGGAG(10/8)
Bsp1286I      421  GAGCCC               G[AGT]GC[ACT]^C
BsrI           53  CCAGT                ACTGG(1/-1)
DpnII         522  GATC                 ^GATC
DraI          706  TTTAAA               TTT^AAA
DraII         342  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII         50  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           93  TGGCCA               [CT]^GGCC[GA]
EcoO109I      342  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          589  CCCGC                CCCGC(4/6)
HapII         298  CCGG                 C^CGG
HpaII         298  CCGG                 C^CGG
MaeIII        667  GTTAC                ^GT[ACGT]AC
MboI          522  GATC                 ^GATC
MluNI          93  TGGCCA               TGG^CCA
MscI           93  TGGCCA               TGG^CCA
MspA1I         97  CAGCTG               C[AC]G^C[GT]G
MspI          298  CCGG                 C^CGG
MwoI          583  GCAACCCCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          298  CCGGG                CC^[GC]GG
NdeII         522  GATC                 ^GATC
NlaIII         41  CATG                 CATG^
NlaIV         132  GGTTCC               GG[ACGT]^[ACGT]CC
PpuMI         342  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          97  CAGCTG               CAG^CTG
RsaI          538  GTAC                 GT^AC
Sau3AI        522  GATC                 ^GATC
SfaNI         595  GCATC                GCATC(5/9)
SfcI          117  CTACAG               C^T[GA][CT]AG
SmlI          162  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          443  CCGC                 CCGC(-3/-1)
              590  CCGC                
AluI           98  AGCT                 AG^CT
              526  AGCT                
BsaJI         198  CCCAGG               C^C[ACGT][ACGT]GG
              199  CCAGGG              
BsmFI         143  GTCCC                GGGAC(10/14)
              196  GTCCC               
BstNI          91  CCTGG                CC^[AT]GG
              199  CCAGG               
Cac8I          95  GCCAGC               GC[ACGT]^[ACGT]GC
              561  GCCTGC              
Cfr13I         21  GGGCC                G^G[ACGT]CC
              343  GGACC               
DdeI          325  CTAAG                C^T[ACGT]AG
              419  CTGAG               
EarI          185  CTCTTC               CTCTTC(1/4)
              546  CTCTTC              
EcoRII         91  CCTGG                ^CC[AT]GG
              199  CCAGG               
Ksp632I       185  CTCTTC               CTCTTC(1/4)
              546  CTCTTC              
MvaI           91  CCTGG                CC^[AT]GG
              199  CCAGG               
Sau96I         21  GGGCC                G^G[ACGT]CC
              343  GGACC               
TaqI          228  TCGA                 T^CGA
              288  TCGA                
TfiI           68  GATTC                G^A[AT]TC
              271  GAATC               
TthHB8I       228  TCGA                 T^CGA
              288  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

BbvI        BssKI       Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MboII       MnlI        MseI        
PleI        ScrFI       Tru9I       TseI        Tsp509I     
TspRI       
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