Restriction map for
pF1KB7371
(Linear) (Six-base) MAPSORT of: KIBB7371.seq Check: 6721 from: 1 to: 948
KIBB7371.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 736 948
Size: 736 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 235 312 948
Size: 104 131 77 636
Fragments arranged by size:
636 131 104 77
AflII C'TTAA_G
Cuts at: 0 85 948
Size: 85 863
AlwNI CAG_nnn'CTG
Cuts at: 0 927 948
Size: 927 21
ApaI G_GGCC'C
Cuts at: 0 610 948
Size: 610 338
AvaI C'yCGr_G
Cuts at: 0 25 948
Size: 25 923
BamHI G'GATC_C
Cuts at: 0 241 948
Size: 241 707
BanI G'GyrC_C
Cuts at: 0 816 948
Size: 816 132
BanII G_rGCy'C
Cuts at: 0 383 610 948
Size: 383 227 338
Fragments arranged by size:
383 338 227
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 264 948
Size: 110 154 684
Fragments arranged by size:
684 154 110
BglII A'GATC_T
Cuts at: 0 479 948
Size: 479 469
Bme1580I G_kGCm'C
Cuts at: 0 574 610 948
Size: 574 36 338
Fragments arranged by size:
574 338 36
BmgBI CAC'GTC
Cuts at: 0 159 948
Size: 159 789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 463 468 948
Size: 367 96 5 480
Fragments arranged by size:
480 367 96 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 504 948
Size: 504 444
BseYI C'CCAG_C
Cuts at: 0 621 948
Size: 621 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 717 948
Size: 447 270 231
Fragments arranged by size:
447 270 231
BsiHKAI G_wGCw'C
Cuts at: 0 26 948
Size: 26 922
Bsp1286I G_dGCh'C
Cuts at: 0 26 383 574 610 948
Size: 26 357 191 36 338
Fragments arranged by size:
357 338 191 36 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 736 948
Size: 736 212
BsrDI GCAATG_nn'
Cuts at: 0 835 937 948
Size: 835 102 11
Fragments arranged by size:
835 102 11
BssSI C'ACGA_G
Cuts at: 0 301 948
Size: 301 647
BstAPI GCAn_nnn'nTGC
Cuts at: 0 927 948
Size: 927 21
BstYI r'GATC_y
Cuts at: 0 241 426 479 533 864 948
Size: 241 185 53 54 331 84
Fragments arranged by size:
331 241 185 84 54 53
BtgI C'CryG_G
Cuts at: 0 253 276 948
Size: 253 23 672
Fragments arranged by size:
672 253 23
DrdI GACnn_nn'nnGTC
Cuts at: 0 415 948
Size: 415 533
EarI CTCTTCn'nnn_
Cuts at: 0 172 200 694 874 948
Size: 172 28 494 180 74
Fragments arranged by size:
494 180 172 74 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 235 312 367 463 468 948
Size: 104 131 77 55 96 5 480
Fragments arranged by size:
480 131 104 96 77 55 5
EcoO109I rG'GnC_Cy
Cuts at: 0 210 408 606 607 749 846 948
Size: 210 198 198 1 142 97 102
Fragments arranged by size:
210 198 198 142 102 97 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 226 258 496 528 948
Size: 1 32 193 32 238 32 420
Fragments arranged by size:
420 238 193 32 32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 948
Size: 541 407
MslI CAynn'nnrTG
Cuts at: 0 547 948
Size: 547 401
NcoI C'CATG_G
Cuts at: 0 253 948
Size: 253 695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 948
Size: 413 32 503
Fragments arranged by size:
503 413 32
PpuMI rG'GwC_Cy
Cuts at: 0 408 749 846 948
Size: 408 341 97 102
Fragments arranged by size:
408 341 102 97
PspOMI G'GGCC_C
Cuts at: 0 606 948
Size: 606 342
SmlI C'TyrA_G
Cuts at: 0 85 948
Size: 85 863
StuI AGG'CCT
Cuts at: 0 151 948
Size: 151 797
StyI C'CwwG_G
Cuts at: 0 253 948
Size: 253 695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 307 948
Size: 307 641
Enzymes that do cut:
AarI AcuI AflII AlwNI ApaI AvaI BamHI BanI
BanII BbsI BglII Bme1580I BmgBI BpmI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstYI
BtgI DrdI EarI Eco57MI EcoO109I Hin4I MmeI MslI
NcoI PpiI PpuMI PspOMI SmlI StuI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI