Restriction map for pF1KB7365
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaBI       BseRI       BsgI        
BsiEI       BsiWI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MscI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        463  TCCGGA               T^CCGGA
AcyI           13  GACGCC               G[GA]^CG[CT]C
AfaI          437  GTAC                 GT^AC
AlwI           60  GATCC                GGATC(4/5)
Asp700I       359  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          41  GTGCTC               G[AT]GC[AT]^C
AvrII          31  CCTAGG               C^CTAGG
BbsI          553  GAAGAC               GAAGAC(2/6)
BbvI          516  GCAGC                GCAGC(8/12)
BlnI           31  CCTAGG               C^CTAGG
BpuAI         553  GAAGAC               GAAGAC(2/6)
BsaHI          13  GACGCC               G[GA]^CG[CT]C
BsaI           27  GAGACC               GGTCTC(1/5)
BsaWI         463  TCCGGA               [AT]^CCGG[AT]
BseAI         463  TCCGGA               T^CCGGA
BsiHKAI        41  GTGCTC               G[AT]GC[AT]^C
BsmBI           8  GAGACG               CGTCTC(1/5)
BspEI         463  TCCGGA               T^CCGGA
BstBI         366  TTCGAA               TT^CGAA
BstF5I        461  CATCC                GGATG(2/0)
BstYI          59  AGATCC               [GA]^GATC[CT]
EcoNI          17  CCTTTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       211  ATGCAT               ATGCA^T
Fnu4HI        516  GCAGC                GC^[ACGT]GC
FokI          461  CATCC                GGATG(9/13)
HapII         464  CCGG                 C^CGG
HgaI           13  GACGC                GACGC(5/10)
Hin1I          13  GACGCC               G[GA]^CG[CT]C
HpaII         464  CCGG                 C^CGG
HphI          522  GGTGA                GGTGA(8/7)
ItaI          516  GCAGC                GC^[ACGT]GC
MaeII         285  ACGT                 A^CGT
MaeIII        263  GTAAC                ^GT[ACGT]AC
MflI           59  AGATCC               [GA]^GATC[CT]
MroI          463  TCCGGA               T^CCGGA
MseI          452  TTAA                 T^TAA
MspA1I        517  CAGCTG               C[AC]G^C[GT]G
MspI          464  CCGG                 C^CGG
MwoI          336  GCCCAAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI          211  ATGCAT               ATGCA^T
NspI          483  ACATGC               [GA]CATG^[CT]
NspV          366  TTCGAA               TT^CGAA
PvuII         517  CAGCTG               CAG^CTG
RsaI          437  GTAC                 GT^AC
SfaNI         385  GCATC                GCATC(5/9)
SfuI          366  TTCGAA               TT^CGAA
Tru9I         452  TTAA                 T^TAA
TseI          516  GCAGC                G^C[AT]GC
XhoII          59  AGATCC               [GA]^GATC[CT]
XmnI          359  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          139  AGCT                 AG^CT
              518  AGCT                
AvaII         466  GGACC                G^G[AT]CC
              491  GGACC               
BfaI           32  CTAG                 C^TAG
              181  CTAG                
BmyI           41  GTGCTC               G[AGT]GC[ACT]^C
              417  GTGCCC              
BsmAI           8  GAGAC                GTCTC(1/5)
               27  GAGAC               
Bsp1286I       41  GTGCTC               G[AGT]GC[ACT]^C
              417  GTGCCC              
Cac8I         305  GCAGGC               GC[ACGT]^[ACGT]GC
              345  GCCAGC              
EarI          153  GAAGAG               CTCTTC(1/4)
              532  GAAGAG              
Ksp632I       153  GAAGAG               CTCTTC(1/4)
              532  GAAGAG              
MaeI           32  CTAG                 C^TAG
              181  CTAG                
TaqI           74  TCGA                 T^CGA
              367  TCGA                
TthHB8I        74  TCGA                 T^CGA
              367  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

BcgI        BpmI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     HaeIII      HinfI       MboI        
MboII       MnlI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       StyI        
TfiI        
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