(Circular) (Six-base) MAPSORT of: pF1KB7360.seq Check: 678 from: 1 to: 4034 pF1KB7360 4034 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 1072 1072 Size: 4034 Acc65I G'GTAC_C Cuts at: 1050 1050 Size: 4034 AclI AA'CG_TT Cuts at: 3874 3874 Size: 4034 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 876 1859 2291 3038 3306 876 Size: 983 432 747 268 1604 Fragments arranged by size: 1604 983 747 432 268 AflIII A'CryG_T Cuts at: 1386 2490 3467 1386 Size: 1104 977 1953 Fragments arranged by size: 1953 1104 977 AgeI A'CCGG_T Cuts at: 2422 2422 Size: 4034 AleI CACnn'nnGTG Cuts at: 685 732 685 Size: 47 3987 AlwNI CAG_nnn'CTG Cuts at: 183 265 395 1339 2906 183 Size: 82 130 944 1567 1311 Fragments arranged by size: 1567 1311 944 130 82 ApaLI G'TGCA_C Cuts at: 2804 2804 Size: 4034 ApoI r'AATT_y Cuts at: 139 231 1038 3387 3643 139 Size: 92 807 2349 256 530 Fragments arranged by size: 2349 807 530 256 92 AseI AT'TA_AT Cuts at: 20 20 Size: 4034 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4034 AvaI C'yCGr_G Cuts at: 1054 1054 Size: 4034 BamHI G'GATC_C Cuts at: 1059 1059 Size: 4034 BanI G'GyrC_C Cuts at: 1050 1464 1712 1747 1050 Size: 414 248 35 3337 Fragments arranged by size: 3337 414 248 35 BanII G_rGCy'C Cuts at: 272 1048 2078 272 Size: 776 1030 2228 Fragments arranged by size: 2228 1030 776 BbeI G_GCGC'C Cuts at: 1468 1716 1468 Size: 248 3786 BbsI GAAGACnn'nnnn_ Cuts at: 699 782 699 Size: 83 3951 BciVI GTATCCnnnnn_n' Cuts at: 1932 2693 4034 1932 Size: 761 1341 1932 Fragments arranged by size: 1932 1341 761 BclI T'GATC_A Cuts at: 1554 1554 Size: 4034 BfrBI ATG'CAT Cuts at: 319 319 Size: 4034 BglII A'GATC_T Cuts at: 1549 3586 1549 Size: 2037 1997 BlpI GC'TnA_GC Cuts at: 1146 1146 Size: 4034 Bme1580I G_kGCm'C Cuts at: 1659 1752 2808 1659 Size: 93 1056 2885 Fragments arranged by size: 2885 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 156 1246 1327 1662 3806 156 Size: 1090 81 335 2144 384 Fragments arranged by size: 2144 1090 384 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 4034 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 152 1210 2089 2581 2879 152 Size: 1058 879 492 298 1307 Fragments arranged by size: 1307 1058 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3738 3738 Size: 4034 BsaAI yAC'GTr Cuts at: 824 1387 824 Size: 563 3471 BsaBI GATnn'nnATC Cuts at: 6 1548 3585 6 Size: 1542 2037 455 Fragments arranged by size: 2037 1542 455 BsaHI Gr'CG_yC Cuts at: 1465 1713 2415 3911 1465 Size: 248 702 1496 1588 Fragments arranged by size: 1588 1496 702 248 BsaWI w'CCGG_w Cuts at: 1223 1744 2422 2696 2843 3254 1223 Size: 521 678 274 147 411 2003 Fragments arranged by size: 2003 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 446 446 Size: 4034 BseYI C'CCAG_C Cuts at: 903 1459 2217 2794 3290 903 Size: 556 758 577 496 1647 Fragments arranged by size: 1647 758 577 556 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 481 657 481 Size: 176 3858 BsiEI CG_ry'CG Cuts at: 104 1576 1622 2830 104 Size: 1472 46 1208 1308 Fragments arranged by size: 1472 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1048 1826 2016 2808 1048 Size: 778 190 792 2274 Fragments arranged by size: 2274 792 778 190 Bsp1286I G_dGCh'C Cuts at: 272 1048 1659 1752 1826 2016 2078 2808 272 Size: 776 611 93 74 190 62 730 1498 Fragments arranged by size: 1498 776 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3254 3254 Size: 4034 BspHI T'CATG_A Cuts at: 512 3210 512 Size: 2698 1336 BspMI ACCTGCnnnn'nnnn_ Cuts at: 535 1084 1298 1600 1981 535 Size: 549 214 302 381 2588 Fragments arranged by size: 2588 549 381 302 214 BsrBI CCG'CTC Cuts at: 50 2326 2380 3864 4029 50 Size: 2276 54 1484 165 55 Fragments arranged by size: 2276 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 542 1946 542 Size: 1404 2630 BsrFI r'CCGG_y Cuts at: 488 2032 2213 2422 488 Size: 1544 181 209 2100 Fragments arranged by size: 2100 1544 209 181 BssSI C'ACGA_G Cuts at: 2305 2663 2305 Size: 358 3676 BstAPI GCAn_nnn'nTGC Cuts at: 1411 1411 Size: 4034 BstBI TT'CG_AA Cuts at: 2395 2395 Size: 4034 BstYI r'GATC_y Cuts at: 1 1059 1526 1549 1884 2130 3142 3228 3240 Size: 1058 467 23 335 246 1012 86 12 Cuts at: 3240 3586 1 Size: 346 449 Fragments arranged by size: 1058 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4034 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 462 1984 2165 2328 462 Size: 1522 181 163 2168 Fragments arranged by size: 2168 1522 181 163 BtsI GCAGTG_nn' Cuts at: 1277 1277 Size: 4034 DraI TTT'AAA Cuts at: 1033 1033 Size: 4034 DraIII CAC_nnn'GTG Cuts at: 936 936 Size: 4034 DrdI GACnn_nn'nnGTC Cuts at: 1740 2598 1740 Size: 858 3176 EaeI y'GGCC_r Cuts at: 1619 1793 2184 2211 3556 1619 Size: 174 391 27 1345 2097 Fragments arranged by size: 2097 1345 391 174 27 EagI C'GGCC_G Cuts at: 1619 1619 Size: 4034 EarI CTCTTCn'nnn_ Cuts at: 931 2057 2267 931 Size: 1126 210 2698 Fragments arranged by size: 2698 1126 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2242 2552 2698 3844 2242 Size: 310 146 1146 2432 Fragments arranged by size: 2432 1146 310 146 EcoICRI GAG'CTC Cuts at: 1046 1046 Size: 4034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 876 1859 2291 3038 3306 876 Size: 983 432 747 268 1604 Fragments arranged by size: 1604 983 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1173 1173 Size: 4034 EcoRI G'AATT_C Cuts at: 139 1038 139 Size: 899 3135 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 4034 FspI TGC'GCA Cuts at: 1815 1815 Size: 4034 HaeII r_GCGC'y Cuts at: 1468 1716 2738 3717 1468 Size: 248 1022 979 1785 Fragments arranged by size: 1785 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 128 150 160 182 349 381 383 415 531 Size: 22 10 22 167 32 2 32 116 Cuts at: 531 563 854 886 1876 1906 1908 1938 128 Size: 32 291 32 990 30 2 30 2224 Fragments arranged by size: 2224 990 291 167 116 32 32 32 32 30 30 22 22 10 2 2 HincII GTy'rAC Cuts at: 1073 1073 Size: 4034 HindIII A'AGCT_T Cuts at: 992 992 Size: 4034 KasI G'GCGC_C Cuts at: 1464 1712 1464 Size: 248 3786 KpnI G_GTAC'C Cuts at: 1054 1054 Size: 4034 MluI A'CGCG_T Cuts at: 3467 3467 Size: 4034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2705 2889 2705 Size: 184 3850 MscI TGG'CCA Cuts at: 1795 1795 Size: 4034 MslI CAynn'nnrTG Cuts at: 685 732 2023 2150 685 Size: 47 1291 127 2569 Fragments arranged by size: 2569 1291 127 47 MspA1I CmG'CkG Cuts at: 627 907 1145 1459 1819 2832 3077 627 Size: 280 238 314 360 1013 245 1584 Fragments arranged by size: 1584 1013 360 314 280 245 238 NaeI GCC'GGC Cuts at: 490 2215 490 Size: 1725 2309 NarI GG'CG_CC Cuts at: 1465 1713 1465 Size: 248 3786 NcoI C'CATG_G Cuts at: 107 107 Size: 4034 NgoMIV G'CCGG_C Cuts at: 488 2213 488 Size: 1725 2309 NsiI A_TGCA'T Cuts at: 321 321 Size: 4034 NspI r_CATG'y Cuts at: 1087 1587 2494 1087 Size: 500 907 2627 Fragments arranged by size: 2627 907 500 PasI CC'CwG_GG Cuts at: 265 265 Size: 4034 PciI A'CATG_T Cuts at: 2490 2490 Size: 4034 PfoI T'CCnGG_A Cuts at: 311 311 Size: 4034 PmeI GTTT'AAAC Cuts at: 1033 1033 Size: 4034 PmlI CAC'GTG Cuts at: 824 824 Size: 4034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3173 3205 3173 Size: 32 4002 PstI C_TGCA'G Cuts at: 1081 1766 1081 Size: 685 3349 PvuI CG_AT'CG Cuts at: 104 104 Size: 4034 PvuII CAG'CTG Cuts at: 627 907 1459 1819 627 Size: 280 552 360 2842 Fragments arranged by size: 2842 552 360 280 RsrII CG'GwC_CG Cuts at: 2229 2229 Size: 4034 SacI G_AGCT'C Cuts at: 1048 1048 Size: 4034 SalI G'TCGA_C Cuts at: 1071 1071 Size: 4034 SapI GCTCTTCn'nnn_ Cuts at: 2057 2267 2057 Size: 210 3824 SbfI CC_TGCA'GG Cuts at: 1081 1081 Size: 4034 ScaI AGT'ACT Cuts at: 3551 3551 Size: 4034 SfcI C'TryA_G Cuts at: 33 387 1077 1762 2755 2946 33 Size: 354 690 685 993 191 1121 Fragments arranged by size: 1121 993 690 685 354 191 SfoI GGC'GCC Cuts at: 1466 1714 1466 Size: 248 3786 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4034 SmaI CCC'GGG Cuts at: 1056 1056 Size: 4034 SmlI C'TyrA_G Cuts at: 167 1189 2104 2596 2858 167 Size: 1022 915 492 262 1343 Fragments arranged by size: 1343 1022 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4034 SphI G_CATG'C Cuts at: 1087 1587 1087 Size: 500 3534 SspI AAT'ATT Cuts at: 206 206 Size: 4034 StyI C'CwwG_G Cuts at: 107 150 426 1168 1522 107 Size: 43 276 742 354 2619 Fragments arranged by size: 2619 742 354 276 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 847 1617 1750 2209 2418 847 Size: 770 133 459 209 2463 Fragments arranged by size: 2463 770 459 209 133 TatI w'GTAC_w Cuts at: 3549 3549 Size: 4034 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3551 3583 3551 Size: 32 4002 Tth111I GACn'n_nGTC Cuts at: 162 1831 162 Size: 1669 2365 XbaI T'CTAG_A Cuts at: 1065 1065 Size: 4034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 582 1529 582 Size: 947 3087 XmaI C'CCGG_G Cuts at: 1054 1054 Size: 4034 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PflMI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI