Restriction map for pF1KB7306
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AlwNI        1690  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1681  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2377  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3051  TGGCCA               TGG^CCA
BbrPI         192  CACGTG               CAC^GTG
BciVI        2313  GGATAC               GTATCC(6/5)
BlpI          592  GCTGAGC              GC^T[ACGT]AGC
BpmI         2277  CTCCAG               CTGGAG(16/14)
Bpu1102I      592  GCTGAGC              GC^T[ACGT]AGC
BsmI          405  GAATGC               GAATGC(1/-1)
Bsp1407I      715  TGTACA               T^GTACA
BspHI        3084  TCATGA               T^CATGA
BsrGI         715  TGTACA               T^GTACA
BssSI        1046  CACGAG               CACGAG(-5/-1)
BstYI        2538  GGATCT               [GA]^GATC[CT]
CelII         592  GCTGAGC              GC^T[ACGT]AGC
DraIII        392  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         3051  TGGCCA               [CT]^GGCC[GA]
EcoNI        2605  CCTGTTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         644  GAATTC               G^AATTC
HgaI          564  GACGC                GACGC(5/10)
MflI         2538  GGATCT               [GA]^GATC[CT]
MluNI        3051  TGGCCA               TGG^CCA
MscI         3051  TGGCCA               TGG^CCA
MslI         1520  CACTTATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1980  CCATGG               C^CATGG
PaeR7I       2366  CTCGAG               C^TCGAG
PflFI        2377  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         733  CCAGTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         192  CACGTG               CAC^GTG
PmlI          192  CACGTG               CAC^GTG
PpuMI         418  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         3084  TCATGA               T^CATGA
SacI          254  GAGCTC               GAGCT^C
SspBI         715  TGTACA               T^GTACA
StuI         1421  AGGCCT               AGG^CCT
Tth111I      2377  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        733  CCAGTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1770  TCTAGA               T^CTAGA
XhoI         2366  CTCGAG               C^TCGAG
XhoII        2538  GGATCT               [GA]^GATC[CT]
XmnI         1681  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII       2317  ACATGT               A^C[GA][CT]GT
             2849  ACATGT              
Alw44I        389  GTGCAC               G^TGCAC
             2809  GTGCAC              
ApaI         1167  GGGCCC               GGGCC^C
             1377  GGGCCC              
ApaLI         389  GTGCAC               G^TGCAC
             2809  GTGCAC              
AvaI         2165  CTCGGG               C^[CT]CG[GA]G
             2366  CTCGAG              
BcnI          416  CCGGG                CC^[GC]GG
             2385  CCGGG               
BsaAI         192  CACGTG               [CT]AC^GT[GA]
             2183  TACGTG              
BsaBI        2297  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2628  GATGCACATC          
BsmAI        1888  GAGAC                GTCTC(1/5)
             1908  GAGAC               
BsoBI        2165  CTCGGG               C^[CT]CG[GA]G
             2366  CTCGAG              
BspLU11I     2317  ACATGT               A^CATGT
             2849  ACATGT              
BsrDI         583  GCAATG               GCAATG(2/0)
             2525  GCAATG              
BstBI        2555  TTCGAA               TT^CGAA
             2756  TTCGAA              
HapII         416  CCGG                 C^CGG
             2385  CCGG                
HpaII         416  CCGG                 C^CGG
             2385  CCGG                
HphI          634  GGTGA                GGTGA(8/7)
             3043  GGTGA               
MaeII         193  ACGT                 A^CGT
             2184  ACGT                
MaeIII       2375  GTGAC                ^GT[ACGT]AC
             3044  GTGAC               
MamI         2297  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2628  GATGCACATC          
MspA1I       2157  CAGCTG               C[AC]G^C[GT]G
             2453  CAGCTG              
MspI          416  CCGG                 C^CGG
             2385  CCGG                
NciI          416  CCGGG                CC^[GC]GG
             2385  CCGGG               
NspV         2555  TTCGAA               TT^CGAA
             2756  TTCGAA              
PvuII        2157  CAGCTG               CAG^CTG
             2453  CAGCTG              
SfuI         2555  TTCGAA               TT^CGAA
             2756  TTCGAA              
SmlI         2366  CTCGAG               C^T[CT][GA]AG
             2927  CTCAAG              
Tsp45I       2375  GTGAC                ^GT[GC]AC
             3044  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BfaI        BglI        BmyI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HindIII     
HinfI       ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XcmI        
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