Restriction map for pF1KB7282
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BcgI        BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaHI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspMI       BsrFI       BssHII      BssSI       BstEII      
BstPI       CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2013  GTATAC               GT^[AC][GT]AC
AclI           83  AACGTT               AA^CGTT
AlwNI        1409  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          580  CCTGAGG              CC^T[ACGT]AGG
BalI         1933  TGGCCA               TGG^CCA
BanI         1941  GGTGCC               G^G[CT][GA]CC
BfaI         2003  CTAG                 C^TAG
BglII         555  AGATCT               A^GATCT
BsaBI         556  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1565  GGTCTC               GGTCTC(1/5)
BsaWI        1028  ACCGGA               [AT]^CCGG[AT]
BsmI         1980  GCATTC               GAATGC(1/-1)
Bsp1407I      611  TGTACA               T^GTACA
BspHI        1785  TCATGA               T^CATGA
BspLU11I     2009  ACATGT               A^CATGT
BsrBI        1333  GAGCGG               CCGCTC(-3/-3)
BsrGI         611  TGTACA               T^GTACA
Bst1107I     2013  GTATAC               GTA^TAC
BstAPI       1456  GCAAAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         555  AGATCT               [GA]^GATC[CT]
BstZ17I      2013  GTATAC               GTA^TAC
Bsu36I        580  CCTGAGG              CC^T[ACGT]AGG
EaeI         1933  TGGCCA               [CT]^GGCC[GA]
Eco81I        580  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2037  GAATTC               G^AATTC
HaeII        1246  AGCGCC               [GA]GCGC^[CT]
HpaI          339  GTTAAC               GTT^AAC
MaeI         2003  CTAG                 C^TAG
MamI          556  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          555  AGATCT               [GA]^GATC[CT]
MluNI        1933  TGGCCA               TGG^CCA
MscI         1933  TGGCCA               TGG^CCA
NruI         1017  TCGCGA               TCG^CGA
PshAI        1157  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I       83  AACGTT               AA^CGTT
RcaI         1785  TCATGA               T^CATGA
ScaI         1894  AGTACT               AGT^ACT
SspBI         611  TGTACA               T^GTACA
SspI          416  AATATT               AAT^ATT
StuI         2093  AGGCCT               AGG^CCT
XcmI         1862  CCACTTCCCGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         555  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII        1018  CGCG                 CG^CG
             2030  CGCG                
AlwI          918  GATCC                GGATC(4/5)
             1224  GATCC               
AvaI          355  CTCGGG               C^[CT]CG[GA]G
              362  CCCGAG              
BbrPI        1560  CACGTG               CAC^GTG
             1956  CACGTG              
BbvCI        1530  CCTCAGC              CCTCAGC(-5/-2)
             1946  CCTCAGC             
BsmBI         463  GAGACG               CGTCTC(1/5)
              870  GAGACG              
BsoBI         355  CTCGGG               C^[CT]CG[GA]G
              362  CCCGAG              
BstBI         758  TTCGAA               TT^CGAA
              838  TTCGAA              
BstUI        1018  CGCG                 CG^CG
             2030  CGCG                
CfoI         1247  GCGC                 GCG^C
             2031  GCGC                
EarI         1491  CTCTTC               CTCTTC(1/4)
             1393  GAAGAG              
EcoNI         576  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1628  CCTGTATGAGG         
EcoT14I      1004  CCATGG               C^C[AT][AT]GG
             1118  CCATGG              
EcoT22I         1  ATGCAT               ATGCA^T
              250  ATGCAT              
FauI         1928  CCCGC                CCCGC(4/6)
             1335  GCGGG               
HhaI         1247  GCGC                 GCG^C
             2031  GCGC                
HinP1I       1247  GCGC                 G^CGC
             2031  GCGC                
HincII        339  GTTAAC               GT[CT]^[GA]AC
             1123  GTTGAC              
HindII        339  GTTAAC               GT[CT]^[GA]AC
             1123  GTTGAC              
Ksp632I      1491  CTCTTC               CTCTTC(1/4)
             1393  GAAGAG              
MvnI         1018  CGCG                 CG^CG
             2030  CGCG                
NcoI         1004  CCATGG               C^CATGG
             1118  CCATGG              
NsiI            1  ATGCAT               ATGCA^T
              250  ATGCAT              
NspI         2009  ACATGT               [GA]CATG^[CT]
             2054  ACATGC              
NspV          758  TTCGAA               TT^CGAA
              838  TTCGAA              
PflMI         942  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1065  CCATCTCTTGG         
PmaCI        1560  CACGTG               CAC^GTG
             1956  CACGTG              
PmlI         1560  CACGTG               CAC^GTG
             1956  CACGTG              
PstI          724  CTGCAG               CTGCA^G
             1379  CTGCAG              
PvuII        1604  CAGCTG               CAG^CTG
             1621  CAGCTG              
SacI         1345  GAGCTC               GAGCT^C
             1833  GAGCTC              
SfuI          758  TTCGAA               TT^CGAA
              838  TTCGAA              
StyI         1004  CCATGG               C^C[AT][AT]GG
             1118  CCATGG              
Van91I        942  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1065  CCATCTCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BciVI       BcnI        BmyI        BpmI        
BpuAI       BsaAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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