Restriction map for pF1KB7282
 (Linear) (Six-base) MAPSORT of: KIBB7282.seq  Check: 7551  from: 1  to: 2115



KIBB7282.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0    2014    2115 
   Size:       2014     101
 
AclI AA'CG_TT

Cuts at:      0      84    2115 
   Size:         84    2031
 
AflIII A'CryG_T

Cuts at:      0    1559    1955    2009    2115 
   Size:       1559     396      54     106

  Fragments arranged by size: 

                1559     396     106      54
 
AleI CACnn'nnGTG

Cuts at:      0     652    1219    1471    1645    2115 
   Size:        652     567     252     174     470

  Fragments arranged by size: 

                 652     567     470     252     174
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1414    2115 
   Size:       1414     701
 
ApoI r'AATT_y

Cuts at:      0     538     863    2037    2115 
   Size:        538     325    1174      78

  Fragments arranged by size: 

                1174     538     325      78
 
AvaI C'yCGr_G

Cuts at:      0     355     362    2115 
   Size:        355       7    1753

  Fragments arranged by size: 

                1753     355       7
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1106    1139    2115 
   Size:       1106      33     976

  Fragments arranged by size: 

                1106     976      33
 
BanI G'GyrC_C

Cuts at:      0    1941    2115 
   Size:       1941     174
 
BanII G_rGCy'C

Cuts at:      0     643    1349    1837    2115 
   Size:        643     706     488     278

  Fragments arranged by size: 

                 706     643     488     278
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     357     959    1016    2115 
   Size:        357     602      57    1099

  Fragments arranged by size: 

                1099     602     357      57
 
BbvCI CC'TCA_GC

Cuts at:      0    1531    1947    2115 
   Size:       1531     416     168

  Fragments arranged by size: 

                1531     416     168
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1107    1667    1699    1781    2115 
   Size:       1107     560      32      82     334

  Fragments arranged by size: 

                1107     560     334      82      32
 
BfrBI ATG'CAT

Cuts at:      0       3     252    2115 
   Size:          3     249    1863

  Fragments arranged by size: 

                1863     249       3
 
BglII A'GATC_T

Cuts at:      0     555    2115 
   Size:        555    1560
 
Bme1580I G_kGCm'C

Cuts at:      0     243    1946    2115 
   Size:        243    1703     169

  Fragments arranged by size: 

                1703     243     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     646    1912    2115 
   Size:        646    1266     203

  Fragments arranged by size: 

                1266     646     203
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1658    1913    1962    2115 
   Size:       1658     255      49     153

  Fragments arranged by size: 

                1658     255     153      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     214     978    1436    2115 
   Size:         37     177     764     458     679

  Fragments arranged by size: 

                 764     679     458     177      37
 
Bpu10I CC'TnA_GC

Cuts at:      0    1531    1947    2115 
   Size:       1531     416     168

  Fragments arranged by size: 

                1531     416     168
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1571    2115 
   Size:       1571     544
 
BsaAI yAC'GTr

Cuts at:      0    1299    1562    1958    2115 
   Size:       1299     263     396     157

  Fragments arranged by size: 

                1299     396     263     157
 
BsaBI GATnn'nnATC

Cuts at:      0     560    2115 
   Size:        560    1555
 
BsaWI w'CCGG_w

Cuts at:      0    1028    2115 
   Size:       1028    1087
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1066    1096    2115 
   Size:       1066      30    1019

  Fragments arranged by size: 

                1066    1019      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     598     633    1090    2115 
   Size:        211     387      35     457    1025

  Fragments arranged by size: 

                1025     457     387     211      35
 
BseYI C'CCAG_C

Cuts at:      0    1181    1252    1594    1602    2115 
   Size:       1181      71     342       8     513

  Fragments arranged by size: 

                1181     513     342      71       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     947    1306    1823    2115 
   Size:        947     359     517     292

  Fragments arranged by size: 

                 947     517     359     292
 
BsiHKAI G_wGCw'C

Cuts at:      0    1028    1304    1349    1837    2054    2115 
   Size:       1028     276      45     488     217      61

  Fragments arranged by size: 

                1028     488     276     217      61      45
 
BsmI GAATG_Cn'

Cuts at:      0    1980    2115 
   Size:       1980     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     457     864    2115 
   Size:        457     407    1251

  Fragments arranged by size: 

                1251     457     407
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     643    1028    1304    1349    1837    1946    2054 
   Size:        243     400     385     276      45     488     109     108

Cuts at:   2054    2115 
   Size:         61

  Fragments arranged by size: 

                 488     400     385     276     243     109     108      61
                  45
 
BspHI T'CATG_A

Cuts at:      0    1785    2115 
   Size:       1785     330
 
BsrBI CCG'CTC

Cuts at:      0    1335    2115 
   Size:       1335     780
 
BsrDI GCAATG_nn'

Cuts at:      0     193     392    1553    2115 
   Size:        193     199    1161     562

  Fragments arranged by size: 

                1161     562     199     193
 
BsrGI T'GTAC_A

Cuts at:      0     611    2115 
   Size:        611    1504
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1462    2115 
   Size:       1462     653
 
BstBI TT'CG_AA

Cuts at:      0     759     839    2115 
   Size:        759      80    1276

  Fragments arranged by size: 

                1276     759      80
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     388     777    1473    2115 
   Size:        388     389     696     642

  Fragments arranged by size: 

                 696     642     389     388
 
BstYI r'GATC_y

Cuts at:      0     555    2115 
   Size:        555    1560
 
BstZ17I GTA'TAC

Cuts at:      0    2015    2115 
   Size:       2015     100
 
Bsu36I CC'TnA_GG

Cuts at:      0     581    2115 
   Size:        581    1534
 
BtgI C'CryG_G

Cuts at:      0     647    1004    1118    2115 
   Size:        647     357     114     997

  Fragments arranged by size: 

                 997     647     357     114
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1034    2115 
   Size:       1034    1081
 
BtsI GCAGTG_nn'

Cuts at:      0     243     404     526    1376    2115 
   Size:        243     161     122     850     739

  Fragments arranged by size: 

                 850     739     243     161     122
 
EaeI y'GGCC_r

Cuts at:      0    1933    2115 
   Size:       1933     182
 
EarI CTCTTCn'nnn_

Cuts at:      0    1388    1497    2115 
   Size:       1388     109     618

  Fragments arranged by size: 

                1388     618     109
 
EcoICRI GAG'CTC

Cuts at:      0    1347    1835    2115 
   Size:       1347     488     280

  Fragments arranged by size: 

                1347     488     280
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     214     978    1436    2115 
   Size:         37     177     764     458     679

  Fragments arranged by size: 

                 764     679     458     177      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     580    1632    2115 
   Size:        580    1052     483

  Fragments arranged by size: 

                1052     580     483
 
EcoO109I rG'GnC_Cy

Cuts at:      0     359    1163    1598    2115 
   Size:        359     804     435     517

  Fragments arranged by size: 

                 804     517     435     359
 
EcoRI G'AATT_C

Cuts at:      0    2037    2115 
   Size:       2037      78
 
HaeII r_GCGC'y

Cuts at:      0    1250    2115 
   Size:       1250     865
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1066    1098    1382    1414    1755    1787    2115 
   Size:       1066      32     284      32     341      32     328

  Fragments arranged by size: 

                1066     341     328     284      32      32      32
 
HincII GTy'rAC

Cuts at:      0     341    1125    2115 
   Size:        341     784     990

  Fragments arranged by size: 

                 990     784     341
 
HpaI GTT'AAC

Cuts at:      0     341    2115 
   Size:        341    1774
 
MscI TGG'CCA

Cuts at:      0    1935    2115 
   Size:       1935     180
 
MslI CAynn'nnrTG

Cuts at:      0     386     397     652    1219    1471    1645    2115 
   Size:        386      11     255     567     252     174     470

  Fragments arranged by size: 

                 567     470     386     255     252     174      11
 
MspA1I CmG'CkG

Cuts at:      0    1130    1606    1623    1931    2115 
   Size:       1130     476      17     308     184

  Fragments arranged by size: 

                1130     476     308     184      17
 
NcoI C'CATG_G

Cuts at:      0    1004    1118    2115 
   Size:       1004     114     997

  Fragments arranged by size: 

                1004     997     114
 
NruI TCG'CGA

Cuts at:      0    1019    2115 
   Size:       1019    1096
 
NsiI A_TGCA'T

Cuts at:      0       5     254    2115 
   Size:          5     249    1861

  Fragments arranged by size: 

                1861     249       5
 
NspI r_CATG'y

Cuts at:      0    2013    2058    2115 
   Size:       2013      45      57

  Fragments arranged by size: 

                2013      57      45
 
PciI A'CATG_T

Cuts at:      0    2009    2115 
   Size:       2009     106
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     948    1071    2115 
   Size:        948     123    1044

  Fragments arranged by size: 

                1044     948     123
 
PfoI T'CCnGG_A

Cuts at:      0    1226    1867    2115 
   Size:       1226     641     248

  Fragments arranged by size: 

                1226     641     248
 
PmlI CAC'GTG

Cuts at:      0    1562    1958    2115 
   Size:       1562     396     157

  Fragments arranged by size: 

                1562     396     157
 
PpuMI rG'GwC_Cy

Cuts at:      0     359    1163    1598    2115 
   Size:        359     804     435     517

  Fragments arranged by size: 

                 804     517     435     359
 
PshAI GACnn'nnGTC

Cuts at:      0    1161    2115 
   Size:       1161     954
 
PstI C_TGCA'G

Cuts at:      0     728    1383    2115 
   Size:        728     655     732

  Fragments arranged by size: 

                 732     728     655
 
PvuII CAG'CTG

Cuts at:      0    1606    1623    2115 
   Size:       1606      17     492

  Fragments arranged by size: 

                1606     492      17
 
SacI G_AGCT'C

Cuts at:      0    1349    1837    2115 
   Size:       1349     488     278

  Fragments arranged by size: 

                1349     488     278
 
SanDI GG'GwC_CC

Cuts at:      0     359    1598    2115 
   Size:        359    1239     517

  Fragments arranged by size: 

                1239     517     359
 
ScaI AGT'ACT

Cuts at:      0    1896    2115 
   Size:       1896     219
 
SfcI C'TryA_G

Cuts at:      0     724    1379    1538    2099    2115 
   Size:        724     655     159     561      16

  Fragments arranged by size: 

                 724     655     561     159      16
 
SspI AAT'ATT

Cuts at:      0     418    2115 
   Size:        418    1697
 
StuI AGG'CCT

Cuts at:      0    2095    2115 
   Size:       2095      20
 
StyI C'CwwG_G

Cuts at:      0    1004    1118    2115 
   Size:       1004     114     997

  Fragments arranged by size: 

                1004     997     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     444    1057    2115 
   Size:        444     613    1058

  Fragments arranged by size: 

                1058     613     444
 
TatI w'GTAC_w

Cuts at:      0     611    1520    1894    2115 
   Size:        611     909     374     221

  Fragments arranged by size: 

                 909     611     374     221
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1869    2115 
   Size:       1869     246
 

 Enzymes that do cut:

     AccI     AclI   AflIII     AleI    AlwNI     ApoI     AvaI     BaeI
     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI    BglII Bme1580I
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     HpaI
     MscI     MslI   MspA1I     NcoI     NruI     NsiI     NspI     PciI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PstI    PvuII     SacI
    SanDI     ScaI     SfcI     SspI     StuI     StyI    TaqII     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI     BcgI     BclI     BglI     BlpI    BmgBI     BmtI
    BpuEI    BsaHI    BsiEI    BsiWI    BspEI    BspMI    BsrFI   BssHII
    BssSI   BstEII     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     PacI     PasI     PmeI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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