Restriction map for pF1KB7271
 (Linear) (Six-base) MAPSORT of: KIBB7271.seq  Check: 3645  from: 1  to: 1485



KIBB7271.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0      52    1106    1485 
   Size:         52    1054     379

  Fragments arranged by size: 

                1054     379      52
 
AflII C'TTAA_G

Cuts at:      0    1180    1485 
   Size:       1180     305
 
AleI CACnn'nnGTG

Cuts at:      0     199    1485 
   Size:        199    1286
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     227    1485 
   Size:        227    1258
 
AlwNI CAG_nnn'CTG

Cuts at:      0     570    1480    1485 
   Size:        570     910       5

  Fragments arranged by size: 

                 910     570       5
 
AvaI C'yCGr_G

Cuts at:      0    1449    1485 
   Size:       1449      36
 
BanI G'GyrC_C

Cuts at:      0    1370    1485 
   Size:       1370     115
 
BanII G_rGCy'C

Cuts at:      0     115     283     393     496     990    1450    1459    1485 
   Size:        115     168     110     103     494     460       9      26

  Fragments arranged by size: 

                 494     460     168     115     110     103      26       9
            
 
BbeI G_GCGC'C

Cuts at:      0    1374    1485 
   Size:       1374     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1162    1485 
   Size:       1162     323
 
BglI GCCn_nnn'nGGC

Cuts at:      0     382    1485 
   Size:        382    1103
 
Bme1580I G_kGCm'C

Cuts at:      0     440    1318    1485 
   Size:        440     878     167

  Fragments arranged by size: 

                 878     440     167
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     494     864    1485 
   Size:        494     370     621

  Fragments arranged by size: 

                 621     494     370
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     349     509     899    1049    1485 
   Size:         43     306     160     390     150     436

  Fragments arranged by size: 

                 436     390     306     160     150      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     905    1485 
   Size:        905     580
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     885    1485 
   Size:        885     600
 
BsaHI Gr'CG_yC

Cuts at:      0    1371    1485 
   Size:       1371     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1291    1321    1485 
   Size:       1291      30     164

  Fragments arranged by size: 

                1291     164      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     260     263    1485 
   Size:        260       3    1222

  Fragments arranged by size: 

                1222     260       3
 
BseYI C'CCAG_C

Cuts at:      0     420     439    1229    1485 
   Size:        420      19     790     256

  Fragments arranged by size: 

                 790     420     256      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731    1031    1485 
   Size:        731     300     454

  Fragments arranged by size: 

                 731     454     300
 
BsiHKAI G_wGCw'C

Cuts at:      0     115     393    1485 
   Size:        115     278    1092

  Fragments arranged by size: 

                1092     278     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     283     393     440     496     990    1318    1450 
   Size:        115     168     110      47      56     494     328     132

Cuts at:   1450    1459    1485 
   Size:          9      26

  Fragments arranged by size: 

                 494     328     168     132     115     110      56      47
                  26       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     758     951    1485 
   Size:        758     193     534

  Fragments arranged by size: 

                 758     534     193
 
BsrBI CCG'CTC

Cuts at:      0     398    1485 
   Size:        398    1087
 
BsrFI r'CCGG_y

Cuts at:      0     533    1485 
   Size:        533     952
 
BsrGI T'GTAC_A

Cuts at:      0     346    1485 
   Size:        346    1139
 
BssSI C'ACGA_G

Cuts at:      0     289    1485 
   Size:        289    1196
 
BstEII G'GTnAC_C

Cuts at:      0     139    1485 
   Size:        139    1346
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1023    1485 
   Size:       1023     462
 
BstYI r'GATC_y

Cuts at:      0     127    1391    1485 
   Size:        127    1264      94

  Fragments arranged by size: 

                1264     127      94
 
Bsu36I CC'TnA_GG

Cuts at:      0     953    1100    1485 
   Size:        953     147     385

  Fragments arranged by size: 

                 953     385     147
 
BtgI C'CryG_G

Cuts at:      0     353    1485 
   Size:        353    1132
 
BtsI GCAGTG_nn'

Cuts at:      0    1350    1485 
   Size:       1350     135
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     645     680    1485 
   Size:        645      35     805

  Fragments arranged by size: 

                 805     645      35
 
EaeI y'GGCC_r

Cuts at:      0     383    1485 
   Size:        383    1102
 
EarI CTCTTCn'nnn_

Cuts at:      0     554    1485 
   Size:        554     931
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     986    1485 
   Size:        986     499
 
EcoICRI GAG'CTC

Cuts at:      0     113     391    1485 
   Size:        113     278    1094

  Fragments arranged by size: 

                1094     278     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     349     509     899    1049    1485 
   Size:         43     306     160     390     150     436

  Fragments arranged by size: 

                 436     390     306     160     150      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     904     979    1485 
   Size:        904      75     506

  Fragments arranged by size: 

                 904     506      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     940    1485 
   Size:        940     545
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      71     583    1179    1485 
   Size:         71     512     596     306

  Fragments arranged by size: 

                 596     512     306      71
 
FspI TGC'GCA

Cuts at:      0    1342    1485 
   Size:       1342     143
 
HaeII r_GCGC'y

Cuts at:      0     922    1374    1485 
   Size:        922     452     111

  Fragments arranged by size: 

                 922     452     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302    1255    1287    1485 
   Size:        270      32     953      32     198

  Fragments arranged by size: 

                 953     270     198      32      32
 
KasI G'GCGC_C

Cuts at:      0    1370    1485 
   Size:       1370     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     501    1485 
   Size:        501     984
 
MscI TGG'CCA

Cuts at:      0     385    1485 
   Size:        385    1100
 
MslI CAynn'nnrTG

Cuts at:      0     199     209    1485 
   Size:        199      10    1276

  Fragments arranged by size: 

                1276     199      10
 
MspA1I CmG'CkG

Cuts at:      0    1229    1485 
   Size:       1229     256
 
NaeI GCC'GGC

Cuts at:      0     535    1485 
   Size:        535     950
 
NarI GG'CG_CC

Cuts at:      0    1371    1485 
   Size:       1371     114
 
NdeI CA'TA_TG

Cuts at:      0       7     206    1485 
   Size:          7     199    1279

  Fragments arranged by size: 

                1279     199       7
 
NgoMIV G'CCGG_C

Cuts at:      0     533    1485 
   Size:        533     952
 
NspI r_CATG'y

Cuts at:      0     347     843    1366    1473    1485 
   Size:        347     496     523     107      12

  Fragments arranged by size: 

                 523     496     347     107      12
 
PasI CC'CwG_GG

Cuts at:      0     981    1485 
   Size:        981     504
 
PfoI T'CCnGG_A

Cuts at:      0      20    1485 
   Size:         20    1465
 
PpuMI rG'GwC_Cy

Cuts at:      0     940    1485 
   Size:        940     545
 
PstI C_TGCA'G

Cuts at:      0     454    1228    1443    1485 
   Size:        454     774     215      42

  Fragments arranged by size: 

                 774     454     215      42
 
PvuII CAG'CTG

Cuts at:      0    1229    1485 
   Size:       1229     256
 
SacI G_AGCT'C

Cuts at:      0     115     393    1485 
   Size:        115     278    1092

  Fragments arranged by size: 

                1092     278     115
 
SapI GCTCTTCn'nnn_

Cuts at:      0     554    1485 
   Size:        554     931
 
SbfI CC_TGCA'GG

Cuts at:      0     454    1485 
   Size:        454    1031
 
SfcI C'TryA_G

Cuts at:      0     450    1224    1439    1485 
   Size:        450     774     215      46

  Fragments arranged by size: 

                 774     450     215      46
 
SfoI GGC'GCC

Cuts at:      0    1372    1485 
   Size:       1372     113
 
SmaI CCC'GGG

Cuts at:      0    1451    1485 
   Size:       1451      34
 
SmlI C'TyrA_G

Cuts at:      0    1180    1485 
   Size:       1180     305
 
SphI G_CATG'C

Cuts at:      0    1366    1473    1485 
   Size:       1366     107      12

  Fragments arranged by size: 

                1366     107      12
 
StyI C'CwwG_G

Cuts at:      0     458    1485 
   Size:        458    1027
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1343    1485 
   Size:       1343     142
 
TatI w'GTAC_w

Cuts at:      0     346    1485 
   Size:        346    1139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     932     964    1485 
   Size:        932      32     521

  Fragments arranged by size: 

                 932     521      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     204    1485 
   Size:        204    1281
 
XmaI C'CCGG_G

Cuts at:      0    1449    1485 
   Size:       1449      36
 
XmnI GAAnn'nnTTC

Cuts at:      0    1216    1485 
   Size:       1216     269
 

 Enzymes that do cut:

     AccI    AflII     AleI     AlfI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BglI Bme1580I     BmrI     BpmI   Bpu10I     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI
     PasI     PfoI    PpuMI     PstI    PvuII     SacI     SapI     SbfI
     SfcI     SfoI     SmaI     SmlI     SphI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NcoI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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