################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### ApaI 28 GGGCCC GGGCC^C AvaI 24 CCCGGG C^[CT]CG[GA]G AvrII 70 CCTAGG C^CTAGG BlnI 70 CCTAGG C^CTAGG BsgI 468 GTGCAG GTGCAG(16/14) BsiYI 18 CCAGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 18 CCAGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 10 GAGAC GTCTC(1/5) BsmFI 151 GGGAC GGGAC(10/14) BsoBI 24 CCCGGG C^[CT]CG[GA]G Bsp1407I 195 TGTACA T^GTACA BsrGI 195 TGTACA T^GTACA BstAPI 574 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 49 GGATG GGATG(2/0) Cac8I 238 GCTGGC GC[ACGT]^[ACGT]GC DraII 27 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 27 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 70 CCTAGG C^C[AT][AT]GG FokI 49 GGATG GGATG(9/13) HaeIII 29 GGCC GG^CC HapII 25 CCGG C^CGG HpaII 25 CCGG C^CGG HphI 531 TCACC GGTGA(8/7) MseI 217 TTAA T^TAA MspI 25 CCGG C^CGG PstI 581 CTGCAG CTGCA^G ScaI 403 AGTACT AGT^ACT SfaNI 190 GCATC GCATC(5/9) SfcI 581 CTGCAG C^T[GA][CT]AG SmaI 24 CCCGGG CCC^GGG SspBI 195 TGTACA T^GTACA StyI 70 CCTAGG C^C[AT][AT]GG Tru9I 217 TTAA T^TAA Tsp45I 370 GTCAC ^GT[GC]AC XmaCI 24 CCCGGG C^CCGGG XmaI 24 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 513 AAATTT [GA]^AATT[CT] 626 AAATTC AfaI 196 GTAC GT^AC 404 GTAC AlwNI 182 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 575 CAGAAGCTG ApoI 513 AAATTT [GA]^AATT[CT] 626 AAATTC BanII 28 GGGCCC G[GA]GC[CT]^C 141 GAGCCC BcnI 24 CCCGG CC^[GC]GG 25 CCGGG BmyI 28 GGGCCC G[AGT]GC[ACT]^C 141 GAGCCC Bsp1286I 28 GGGCCC G[AGT]GC[ACT]^C 141 GAGCCC BssKI 24 CCCGG ^CC[ACGT]GG 25 CCGGG Cfr13I 28 GGGCC G^G[ACGT]CC 29 GGCCC HinfI 184 GATTC G^A[ACGT]TC 603 GAATC MboII 418 GAAGA GAAGA(8/7) 111 TCTTC MwoI 134 GCAGAATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 574 GCAGAAGCTGC NciI 24 CCCGG CC^[GC]GG 25 CCGGG NlaIII 33 CATG CATG^ 465 CATG RsaI 196 GTAC GT^AC 404 GTAC Sau96I 28 GGGCC G^G[ACGT]CC 29 GGCCC ScrFI 24 CCCGG CC^[ACGT]GG 25 CCGGG TfiI 184 GATTC G^A[AT]TC 603 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI Asp700I BbvI BfaI BsaJI BsrI DdeI Fnu4HI ItaI MaeI MaeIII MnlI NlaIV TseI Tsp509I XmnI