(Circular) (Six-base) MAPSORT of: pF1KB7270.seq Check: 9650 from: 1 to: 3752 pF1KB7270 3752 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 790 790 Size: 3752 Acc65I G'GTAC_C Cuts at: 768 768 Size: 3752 AclI AA'CG_TT Cuts at: 3592 3592 Size: 3752 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 205 1577 2009 2756 3024 205 Size: 1372 432 747 268 933 Fragments arranged by size: 1372 933 747 432 268 AflIII A'CryG_T Cuts at: 1104 2208 3185 1104 Size: 1104 977 1671 Fragments arranged by size: 1671 1104 977 AgeI A'CCGG_T Cuts at: 2140 2140 Size: 3752 AlwNI CAG_nnn'CTG Cuts at: 295 688 1057 2624 295 Size: 393 369 1567 1423 Fragments arranged by size: 1567 1423 393 369 ApaI G_GGCC'C Cuts at: 140 140 Size: 3752 ApaLI G'TGCA_C Cuts at: 2522 2522 Size: 3752 ApoI r'AATT_y Cuts at: 621 734 756 3105 3361 621 Size: 113 22 2349 256 1012 Fragments arranged by size: 2349 1012 256 113 22 AseI AT'TA_AT Cuts at: 20 20 Size: 3752 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3752 AvaI C'yCGr_G Cuts at: 132 772 132 Size: 640 3112 AvrII C'CTAG_G Cuts at: 178 178 Size: 3752 BamHI G'GATC_C Cuts at: 777 777 Size: 3752 BanI G'GyrC_C Cuts at: 768 1182 1430 1465 768 Size: 414 248 35 3055 Fragments arranged by size: 3055 414 248 35 BanII G_rGCy'C Cuts at: 140 253 766 1796 140 Size: 113 513 1030 2096 Fragments arranged by size: 2096 1030 513 113 BbeI G_GCGC'C Cuts at: 1186 1434 1186 Size: 248 3504 BciVI GTATCCnnnnn_n' Cuts at: 1650 2411 3752 1650 Size: 761 1341 1650 Fragments arranged by size: 1650 1341 761 BclI T'GATC_A Cuts at: 1272 1272 Size: 3752 BglII A'GATC_T Cuts at: 1267 3304 1267 Size: 2037 1715 BlpI GC'TnA_GC Cuts at: 864 864 Size: 3752 Bme1580I G_kGCm'C Cuts at: 140 1377 1470 2526 140 Size: 1237 93 1056 1366 Fragments arranged by size: 1366 1237 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 482 503 964 1045 1380 3524 482 Size: 21 461 81 335 2144 710 Fragments arranged by size: 2144 710 461 335 81 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2119 2119 Size: 3752 Bpu10I CC'TnA_GC Cuts at: 113 113 Size: 3752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 928 1807 2299 2597 928 Size: 879 492 298 2083 Fragments arranged by size: 2083 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3456 3456 Size: 3752 BsaAI yAC'GTr Cuts at: 1105 1105 Size: 3752 BsaBI GATnn'nnATC Cuts at: 6 1266 3303 6 Size: 1260 2037 455 Fragments arranged by size: 2037 1260 455 BsaHI Gr'CG_yC Cuts at: 1183 1431 2133 3629 1183 Size: 248 702 1496 1306 Fragments arranged by size: 1496 1306 702 248 BsaWI w'CCGG_w Cuts at: 941 1462 2140 2414 2561 2972 941 Size: 521 678 274 147 411 1721 Fragments arranged by size: 1721 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1177 1935 2512 3008 1177 Size: 758 577 496 1921 Fragments arranged by size: 1921 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 597 597 Size: 3752 BsiEI CG_ry'CG Cuts at: 104 1294 1340 2548 104 Size: 1190 46 1208 1308 Fragments arranged by size: 1308 1208 1190 46 BsiHKAI G_wGCw'C Cuts at: 766 1544 1734 2526 766 Size: 778 190 792 1992 Fragments arranged by size: 1992 792 778 190 Bsp1286I G_dGCh'C Cuts at: 140 253 766 1377 1470 1544 1734 1796 2526 Size: 113 513 611 93 74 190 62 730 Cuts at: 2526 140 Size: 1366 Fragments arranged by size: 1366 730 611 513 190 113 93 74 62 BspEI T'CCGG_A Cuts at: 2972 2972 Size: 3752 BspHI T'CATG_A Cuts at: 2928 2928 Size: 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 802 1016 1318 1699 802 Size: 214 302 381 2855 Fragments arranged by size: 2855 381 302 214 BsrBI CCG'CTC Cuts at: 50 2044 2098 3582 3747 50 Size: 1994 54 1484 165 55 Fragments arranged by size: 1994 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1664 1664 Size: 3752 BsrFI r'CCGG_y Cuts at: 1750 1931 2140 1750 Size: 181 209 3362 Fragments arranged by size: 3362 209 181 BsrGI T'GTAC_A Cuts at: 303 303 Size: 3752 BssSI C'ACGA_G Cuts at: 2023 2381 2023 Size: 358 3394 BstAPI GCAn_nnn'nTGC Cuts at: 688 1129 688 Size: 441 3311 BstBI TT'CG_AA Cuts at: 2113 2113 Size: 3752 BstYI r'GATC_y Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958 Size: 776 467 23 335 246 1012 86 12 Cuts at: 2958 3304 1 Size: 346 449 Fragments arranged by size: 1012 776 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3752 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1702 1883 2046 1702 Size: 181 163 3408 Fragments arranged by size: 3408 181 163 BtsI GCAGTG_nn' Cuts at: 995 995 Size: 3752 DraI TTT'AAA Cuts at: 751 751 Size: 3752 DrdI GACnn_nn'nnGTC Cuts at: 1458 2316 1458 Size: 858 2894 EaeI y'GGCC_r Cuts at: 1337 1511 1902 1929 3274 1337 Size: 174 391 27 1345 1815 Fragments arranged by size: 1815 1345 391 174 27 EagI C'GGCC_G Cuts at: 1337 1337 Size: 3752 EarI CTCTTCn'nnn_ Cuts at: 1775 1985 1775 Size: 210 3542 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1960 2270 2416 3562 1960 Size: 310 146 1146 2150 Fragments arranged by size: 2150 1146 310 146 EcoICRI GAG'CTC Cuts at: 764 764 Size: 3752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 205 1577 2009 2756 3024 205 Size: 1372 432 747 268 933 Fragments arranged by size: 1372 933 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 136 891 136 Size: 755 2997 EcoRI G'AATT_C Cuts at: 756 756 Size: 3752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 235 610 1771 235 Size: 375 1161 2216 Fragments arranged by size: 2216 1161 375 FspI TGC'GCA Cuts at: 1533 1533 Size: 3752 HaeII r_GCGC'y Cuts at: 1186 1434 2456 3435 1186 Size: 248 1022 979 1503 Fragments arranged by size: 1503 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 283 315 1594 1624 1626 1656 283 Size: 32 1279 30 2 30 2379 Fragments arranged by size: 2379 1279 32 30 30 2 HincII GTy'rAC Cuts at: 791 791 Size: 3752 KasI G'GCGC_C Cuts at: 1182 1430 1182 Size: 248 3504 KpnI G_GTAC'C Cuts at: 772 772 Size: 3752 MluI A'CGCG_T Cuts at: 3185 3185 Size: 3752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 299 2423 2607 299 Size: 2124 184 1444 Fragments arranged by size: 2124 1444 184 MscI TGG'CCA Cuts at: 1513 1513 Size: 3752 MslI CAynn'nnrTG Cuts at: 1741 1868 1741 Size: 127 3625 MspA1I CmG'CkG Cuts at: 863 1177 1537 2550 2795 863 Size: 314 360 1013 245 1820 Fragments arranged by size: 1820 1013 360 314 245 NaeI GCC'GGC Cuts at: 1933 1933 Size: 3752 NarI GG'CG_CC Cuts at: 1183 1431 1183 Size: 248 3504 NcoI C'CATG_G Cuts at: 107 107 Size: 3752 NgoMIV G'CCGG_C Cuts at: 1931 1931 Size: 3752 NspI r_CATG'y Cuts at: 805 1305 2212 805 Size: 500 907 2345 Fragments arranged by size: 2345 907 500 PciI A'CATG_T Cuts at: 2208 2208 Size: 3752 PmeI GTTT'AAAC Cuts at: 751 751 Size: 3752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2891 2923 2891 Size: 32 3720 PspOMI G'GGCC_C Cuts at: 136 136 Size: 3752 PstI C_TGCA'G Cuts at: 693 799 1484 693 Size: 106 685 2961 Fragments arranged by size: 2961 685 106 PvuI CG_AT'CG Cuts at: 104 104 Size: 3752 PvuII CAG'CTG Cuts at: 1177 1537 1177 Size: 360 3392 RsrII CG'GwC_CG Cuts at: 1947 1947 Size: 3752 SacI G_AGCT'C Cuts at: 766 766 Size: 3752 SalI G'TCGA_C Cuts at: 789 789 Size: 3752 SapI GCTCTTCn'nnn_ Cuts at: 1775 1985 1775 Size: 210 3542 SbfI CC_TGCA'GG Cuts at: 799 799 Size: 3752 ScaI AGT'ACT Cuts at: 513 3269 513 Size: 2756 996 SfcI C'TryA_G Cuts at: 33 689 795 1480 2473 2664 33 Size: 656 106 685 993 191 1121 Fragments arranged by size: 1121 993 685 656 191 106 SfoI GGC'GCC Cuts at: 1184 1432 1184 Size: 248 3504 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3752 SmaI CCC'GGG Cuts at: 134 774 134 Size: 640 3112 SmlI C'TyrA_G Cuts at: 907 1822 2314 2576 907 Size: 915 492 262 2083 Fragments arranged by size: 2083 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3752 SphI G_CATG'C Cuts at: 805 1305 805 Size: 500 3252 StyI C'CwwG_G Cuts at: 107 178 886 1240 107 Size: 71 708 354 2619 Fragments arranged by size: 2619 708 354 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1335 1468 1927 2136 1335 Size: 133 459 209 2951 Fragments arranged by size: 2951 459 209 133 TatI w'GTAC_w Cuts at: 303 511 3267 303 Size: 208 2756 788 Fragments arranged by size: 2756 788 208 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 252 284 3269 3301 252 Size: 32 2985 32 703 Fragments arranged by size: 2985 703 32 32 Tth111I GACn'n_nGTC Cuts at: 1549 1549 Size: 3752 XbaI T'CTAG_A Cuts at: 783 783 Size: 3752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1247 1247 Size: 3752 XmaI C'CCGG_G Cuts at: 132 772 132 Size: 640 3112 XmnI GAAnn'nnTTC Cuts at: 195 218 734 195 Size: 23 516 3213 Fragments arranged by size: 3213 516 23 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI