Restriction map for pF1KB7263
 (Linear) (Six-base) MAPSORT of: KIBB7263.seq  Check: 8361  from: 1  to: 945



KIBB7263.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     232     945 
   Size:        232     713
 
AflIII A'CryG_T

Cuts at:      0     724     945 
   Size:        724     221
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     309     945 
   Size:        309     636
 
ApoI r'AATT_y

Cuts at:      0     911     945 
   Size:        911      34
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     713     746     945 
   Size:        713      33     199

  Fragments arranged by size: 

                 713     199      33
 
BanII G_rGCy'C

Cuts at:      0      35     945 
   Size:         35     910
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     741     945 
   Size:        741     204
 
BlpI GC'TnA_GC

Cuts at:      0     302     945 
   Size:        302     643
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     853     945 
   Size:        853      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     257     355     945 
   Size:         85     172      98     590

  Fragments arranged by size: 

                 590     172      98      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     945 
   Size:        379     566
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     945 
   Size:         35     910
 
BsrDI GCAATG_nn'

Cuts at:      0     525     614     945 
   Size:        525      89     331

  Fragments arranged by size: 

                 525     331      89
 
BsrGI T'GTAC_A

Cuts at:      0     571     945 
   Size:        571     374
 
BssSI C'ACGA_G

Cuts at:      0     695     945 
   Size:        695     250
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     796     945 
   Size:        796     149
 
BstYI r'GATC_y

Cuts at:      0     278     945 
   Size:        278     667
 
Bsu36I CC'TnA_GG

Cuts at:      0      46     931     945 
   Size:         46     885      14

  Fragments arranged by size: 

                 885      46      14
 
BtgI C'CryG_G

Cuts at:      0     356     513     945 
   Size:        356     157     432

  Fragments arranged by size: 

                 432     356     157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     775     945 
   Size:        775     170
 
BtsI GCAGTG_nn'

Cuts at:      0     212     391     945 
   Size:        212     179     554

  Fragments arranged by size: 

                 554     212     179
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     231     266     945 
   Size:        231      35     679

  Fragments arranged by size: 

                 679     231      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     628     945 
   Size:        628     317
 
EaeI y'GGCC_r

Cuts at:      0     353     945 
   Size:        353     592
 
EarI CTCTTCn'nnn_

Cuts at:      0     100     695     945 
   Size:        100     595     250

  Fragments arranged by size: 

                 595     250     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     257     355     945 
   Size:         85     172      98     590

  Fragments arranged by size: 

                 590     172      98      85
 
EcoRI G'AATT_C

Cuts at:      0     911     945 
   Size:        911      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     486     518     676     708     945 
   Size:        486      32     158      32     237

  Fragments arranged by size: 

                 486     237     158      32      32
 
HindIII A'AGCT_T

Cuts at:      0     459     945 
   Size:        459     486
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     945 
   Size:        245     700
 
MscI TGG'CCA

Cuts at:      0     355     945 
   Size:        355     590
 
MslI CAynn'nnrTG

Cuts at:      0     518     551     740     829     945 
   Size:        518      33     189      89     116

  Fragments arranged by size: 

                 518     189     116      89      33
 
MspA1I CmG'CkG

Cuts at:      0     306     945 
   Size:        306     639
 
NcoI C'CATG_G

Cuts at:      0     356     513     945 
   Size:        356     157     432

  Fragments arranged by size: 

                 432     356     157
 
NspI r_CATG'y

Cuts at:      0     728     945 
   Size:        728     217
 
PciI A'CATG_T

Cuts at:      0     724     945 
   Size:        724     221
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     772     945 
   Size:        772     173
 
PfoI T'CCnGG_A

Cuts at:      0      57     332     945 
   Size:         57     275     613

  Fragments arranged by size: 

                 613     275      57
 
PvuII CAG'CTG

Cuts at:      0     306     945 
   Size:        306     639
 
SapI GCTCTTCn'nnn_

Cuts at:      0     695     945 
   Size:        695     250
 
SfcI C'TryA_G

Cuts at:      0     879     945 
   Size:        879      66
 
StyI C'CwwG_G

Cuts at:      0     356     513     945 
   Size:        356     157     432

  Fragments arranged by size: 

                 432     356     157
 
TatI w'GTAC_w

Cuts at:      0     471     571     945 
   Size:        471     100     374

  Fragments arranged by size: 

                 471     374     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       3      35     945 
   Size:          3      32     910

  Fragments arranged by size: 

                 910      32       3
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     627     945 
   Size:        627     318
 

 Enzymes that do cut:

     AccI   AflIII     AlfI     ApoI     BaeI    BanII    BciVI     BlpI
     BmrI     BpmI     BsgI Bsp1286I    BsrDI    BsrGI    BssSI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EarI  Eco57MI    EcoRI    Hin4I  HindIII     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PciI    PflMI     PfoI    PvuII     SapI
     SfcI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I     ClaI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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