Restriction map for pF1KB7262
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BbrPI       BcgI        BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII          42  CGCG                 CG^CG
AcyI          196  GGCGCC               G[GA]^CG[CT]C
AflIII         89  ACATGT               A^C[GA][CT]GT
AocI          589  CCTCAGG              CC^T[ACGT]AGG
ApaI          314  GGGCCC               GGGCC^C
Asp700I      1276  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          963  TGGCCA               TGG^CCA
BamHI         594  GGATCC               G^GATCC
BbeI          196  GGCGCC               GGCGC^C
BbvCI         654  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1030  GTATCC               GTATCC(6/5)
BfaI         1286  CTAG                 C^TAG
BpmI         1298  CTGGAG               CTGGAG(16/14)
BsaHI         196  GGCGCC               G[GA]^CG[CT]C
BseRI          24  CTCCTC               GAGGAG(10/8)
BsmAI         582  GAGAC                GTCTC(1/5)
BspLU11I       89  ACATGT               A^CATGT
BsrDI         267  GCAATG               GCAATG(2/0)
BssSI        1072  CACGAG               CACGAG(-5/-1)
BstUI          42  CGCG                 CG^CG
BstXI         278  CCAAGTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        589  CCTCAGG              CC^T[ACGT]AGG
Eco81I        589  CCTCAGG              CC^T[ACGT]AGG
HgaI         1053  GCGTC                GACGC(5/10)
Hin1I         196  GGCGCC               G[GA]^CG[CT]C
KasI          196  GGCGCC               G^GCGCC
MaeI         1286  CTAG                 C^TAG
MluNI         963  TGGCCA               TGG^CCA
MscI          963  TGGCCA               TGG^CCA
MslI          263  CACAGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           42  CGCG                 CG^CG
NarI          196  GGCGCC               GG^CGCC
NspI           89  ACATGT               [GA]CATG^[CT]
PaeR7I         74  CTCGAG               C^TCGAG
PflMI        1291  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          420  CTGCAG               CTGCA^G
PvuII         423  CAGCTG               CAG^CTG
SexAI         985  ACCAGGT              A^CC[AT]GGT
SfcI          420  CTGCAG               C^T[GA][CT]AG
SfoI          196  GGCGCC               GGC^GCC
SmaI          630  CCCGGG               CCC^GGG
SmlI           74  CTCGAG               C^T[CT][GA]AG
TaqI           75  TCGA                 T^CGA
TfiI         1176  GAATC                G^A[AT]TC
TthHB8I        75  TCGA                 T^CGA
Van91I       1291  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           74  CTCGAG               C^TCGAG
XmaCI         630  CCCGGG               C^CCGGG
XmaI          630  CCCGGG               C^CCGGG
XmnI         1276  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwI          595  GATCC                GGATC(4/5)
              594  GGATC               
AlwNI         956  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1292  CAGACCCTG           
BanII          77  GAGCCC               G[GA]GC[CT]^C
              314  GGGCCC              
BbsI          768  GAAGAC               GAAGAC(2/6)
             1280  GTCTTC              
BpuAI         768  GAAGAC               GAAGAC(2/6)
             1280  GTCTTC              
BsgI          426  CTGCAC               GTGCAG(16/14)
              562  GTGCAG              
BstAPI        830  GCATCCTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              932  GCAAGATTTGC         
EarI          111  GAAGAG               CTCTTC(1/4)
              550  GAAGAG              
HaeII         196  GGCGCC               [GA]GCGC^[CT]
              658  AGCGCC              
Ksp632I       111  GAAGAG               CTCTTC(1/4)
              550  GAAGAG              
MaeII         772  ACGT                 A^CGT
             1278  ACGT                
MspA1I        423  CAGCTG               C[AC]G^C[GT]G
              493  CCGCTG              
NcoI          105  CCATGG               C^CATGG
              573  CCATGG              
PpuMI          17  GGGTCCT              [GA]G^G[AT]CC[CT]
             1010  GGGTCCT             
SfaNI         610  GATGC                GCATC(5/9)
              830  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanI        BbvI        BcnI        BglII       BmyI        
BsaJI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       StuI        StyI        TseI        Tsp45I      
TspRI       XhoII       
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