Restriction map for pF1KB7262
 (Linear) (Six-base) MAPSORT of: KIBB7262.seq  Check: 2697  from: 1  to: 1353



KIBB7262.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     672     751    1325    1353 
   Size:        354     318      79     574      28

  Fragments arranged by size: 

                 574     354     318      79      28
 
AflIII A'CryG_T

Cuts at:      0      89    1353 
   Size:         89    1264
 
AlwNI CAG_nnn'CTG

Cuts at:      0     961    1297    1353 
   Size:        961     336      56

  Fragments arranged by size: 

                 961     336      56
 
ApaI G_GGCC'C

Cuts at:      0     318    1353 
   Size:        318    1035
 
AvaI C'yCGr_G

Cuts at:      0       7      74     630     907    1353 
   Size:          7      67     556     277     446

  Fragments arranged by size: 

                 556     446     277      67       7
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1013    1046    1353 
   Size:       1013      33     307

  Fragments arranged by size: 

                1013     307      33
 
BamHI G'GATC_C

Cuts at:      0     594    1353 
   Size:        594     759
 
BanI G'GyrC_C

Cuts at:      0     137     196     634     794     999    1353 
   Size:        137      59     438     160     205     354

  Fragments arranged by size: 

                 438     354     205     160     137      59
 
BanII G_rGCy'C

Cuts at:      0      81     318    1353 
   Size:         81     237    1035

  Fragments arranged by size: 

                1035     237      81
 
BbeI G_GCGC'C

Cuts at:      0     200    1353 
   Size:        200    1153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     775    1273    1353 
   Size:        775     498      80

  Fragments arranged by size: 

                 775     498      80
 
BbvCI CC'TCA_GC

Cuts at:      0     655    1353 
   Size:        655     698
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1041    1353 
   Size:       1041     312
 
BglII A'GATC_T

Cuts at:      0     365     440    1094    1353 
   Size:        365      75     654     259

  Fragments arranged by size: 

                 654     365     259      75
 
Bme1580I G_kGCm'C

Cuts at:      0     318     378     637    1353 
   Size:        318      60     259     716

  Fragments arranged by size: 

                 716     318     259      60
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     605    1353 
   Size:        605     748
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1319    1353 
   Size:       1319      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     655    1353 
   Size:        655     698
 
BsaHI Gr'CG_yC

Cuts at:      0     197    1353 
   Size:        197    1156
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      15    1353 
   Size:         15    1338
 
BseYI C'CCAG_C

Cuts at:      0      33     245     480     613     639     919    1353 
   Size:         33     212     235     133      26     280     434

  Fragments arranged by size: 

                 434     280     235     212     133      33      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     583    1353 
   Size:        411     172     770

  Fragments arranged by size: 

                 770     411     172
 
Bsp1286I G_dGCh'C

Cuts at:      0      81     318     378     637    1353 
   Size:         81     237      60     259     716

  Fragments arranged by size: 

                 716     259     237      81      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     193     469    1058    1353 
   Size:        193     276     589     295

  Fragments arranged by size: 

                 589     295     276     193
 
BsrDI GCAATG_nn'

Cuts at:      0     274    1353 
   Size:        274    1079
 
BssSI C'ACGA_G

Cuts at:      0    1072    1353 
   Size:       1072     281
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     836     938    1353 
   Size:        836     102     415

  Fragments arranged by size: 

                 836     415     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     285    1353 
   Size:        285    1068
 
BstYI r'GATC_y

Cuts at:      0     365     440     594    1094    1353 
   Size:        365      75     154     500     259

  Fragments arranged by size: 

                 500     365     259     154      75
 
Bsu36I CC'TnA_GG

Cuts at:      0     590    1353 
   Size:        590     763
 
BtgI C'CryG_G

Cuts at:      0     105     573     780    1353 
   Size:        105     468     207     573

  Fragments arranged by size: 

                 573     468     207     105
 
BtsI GCAGTG_nn'

Cuts at:      0     237    1353 
   Size:        237    1116
 
EaeI y'GGCC_r

Cuts at:      0     852     963    1314    1353 
   Size:        852     111     351      39

  Fragments arranged by size: 

                 852     351     111      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     106     545    1353 
   Size:        106     439     808

  Fragments arranged by size: 

                 808     439     106
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     218    1353 
   Size:        218    1135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     672     751    1319    1325    1353 
   Size:        354     318      79     568       6      28

  Fragments arranged by size: 

                 568     354     318      79      28       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     315    1011    1353 
   Size:         18     297     696     342

  Fragments arranged by size: 

                 696     342     297      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1234    1353 
   Size:       1234     119
 
HaeII r_GCGC'y

Cuts at:      0     200     662    1353 
   Size:        200     462     691

  Fragments arranged by size: 

                 691     462     200
 
KasI G'GCGC_C

Cuts at:      0     196    1353 
   Size:        196    1157
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    1353 
   Size:       1267      86
 
MscI TGG'CCA

Cuts at:      0     965    1353 
   Size:        965     388
 
MslI CAynn'nnrTG

Cuts at:      0     267    1353 
   Size:        267    1086
 
MspA1I CmG'CkG

Cuts at:      0     425     495    1353 
   Size:        425      70     858

  Fragments arranged by size: 

                 858     425      70
 
NarI GG'CG_CC

Cuts at:      0     197    1353 
   Size:        197    1156
 
NcoI C'CATG_G

Cuts at:      0     105     573    1353 
   Size:        105     468     780

  Fragments arranged by size: 

                 780     468     105
 
NspI r_CATG'y

Cuts at:      0      93    1353 
   Size:         93    1260
 
PciI A'CATG_T

Cuts at:      0      89    1353 
   Size:         89    1264
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1297    1353 
   Size:       1297      56
 
PpuMI rG'GwC_Cy

Cuts at:      0      18    1011    1353 
   Size:         18     993     342

  Fragments arranged by size: 

                 993     342      18
 
PspOMI G'GGCC_C

Cuts at:      0     314    1353 
   Size:        314    1039
 
PspXI vC'TCGA_Gb

Cuts at:      0      74    1353 
   Size:         74    1279
 
PstI C_TGCA'G

Cuts at:      0     424    1353 
   Size:        424     929
 
PvuII CAG'CTG

Cuts at:      0     425    1353 
   Size:        425     928
 
SexAI A'CCwGG_T

Cuts at:      0     985    1353 
   Size:        985     368
 
SfcI C'TryA_G

Cuts at:      0     420    1353 
   Size:        420     933
 
SfoI GGC'GCC

Cuts at:      0     198    1353 
   Size:        198    1155
 
SmaI CCC'GGG

Cuts at:      0     632    1353 
   Size:        632     721
 
SmlI C'TyrA_G

Cuts at:      0      74    1353 
   Size:         74    1279
 
SrfI GCCC'GGGC

Cuts at:      0     632    1353 
   Size:        632     721
 
StuI AGG'CCT

Cuts at:      0     161     517    1078    1353 
   Size:        161     356     561     275

  Fragments arranged by size: 

                 561     356     275     161
 
StyI C'CwwG_G

Cuts at:      0     105     573     695     995    1159    1353 
   Size:        105     468     122     300     164     194

  Fragments arranged by size: 

                 468     300     194     164     122     105
 
TatI w'GTAC_w

Cuts at:      0     167    1353 
   Size:        167    1186
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1224    1256    1303    1335    1353 
   Size:       1224      32      47      32      18

  Fragments arranged by size: 

                1224      47      32      32      18
 
XhoI C'TCGA_G

Cuts at:      0      74    1353 
   Size:         74    1279
 
XmaI C'CCGG_G

Cuts at:      0     630    1353 
   Size:        630     723
 
XmnI GAAnn'nnTTC

Cuts at:      0    1280    1353 
   Size:       1280      73
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApaI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI    BglII Bme1580I     BplI
     BpmI   Bpu10I    BsaHI    BseRI    BseYI     BsgI Bsp1286I    BspMI
    BsrDI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI
    PflMI    PpuMI   PspOMI    PspXI     PstI    PvuII    SexAI     SfcI
     SfoI     SmaI     SmlI     SrfI     StuI     StyI     TatI     TstI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI
     ZraI
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