Restriction map for pF1KB7248
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BamHI       BbrPI       BciVI       BclI        
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BspDI       BspEI       BsrDI       BssHII      Bst1107I    
BstZ17I     Bsu36I      CelII       ClaI        DraI        
DsaI        Eam1105I    Eco81I      EcoRI       EcoT22I     
FbaI        HincII      HindII      HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        SacII       
SalI        SbfI        ScaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AcsI          997  GAATTT               [GA]^AATT[CT]
Alw44I         97  GTGCAC               G^TGCAC
Aor51HI       759  AGCGCT               AGC^GCT
ApaLI          97  GTGCAC               G^TGCAC
ApoI          997  GAATTT               [GA]^AATT[CT]
Asp700I        87  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          438  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         308  TGCGCA               TGC^GCA
BalI          598  TGGCCA               TGG^CCA
BanI           72  GGCGCC               G^G[CT][GA]CC
BbeI           72  GGCGCC               GGCGC^C
BbsI         1153  GAAGAC               GAAGAC(2/6)
BbvCI         111  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1494  CGAAAGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1050  AGATCT               A^GATCT
BpuAI        1153  GAAGAC               GAAGAC(2/6)
BsaI         1507  GAGACC               GGTCTC(1/5)
BsgI         1502  GTGCAG               GTGCAG(16/14)
BsiEI        1237  CGGCCG               CG[GA][CT]^CG
BsmI          670  GCATTC               GAATGC(1/-1)
BspHI         143  TCATGA               T^CATGA
BspLU11I     1157  ACATGT               A^CATGT
BsrBI        1179  GAGCGG               CCGCTC(-3/-3)
BstBI        1492  TTCGAA               TT^CGAA
BstEII       1315  GGTGACC              G^GT[ACGT]ACC
BstPI        1315  GGTGACC              G^GT[ACGT]ACC
BstXI        1259  CCATCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1050  AGATCT               [GA]^GATC[CT]
CpoI          164  CGGTCCG              CG^G[AT]CCG
EagI         1237  CGGCCG               C^GGCCG
EclXI        1237  CGGCCG               C^GGCCG
Eco47III      759  AGCGCT               AGC^GCT
Eco52I       1237  CGGCCG               C^GGCCG
EcoO65I      1315  GGTGACC              G^GT[ACGT]ACC
EcoRV         710  GATATC               GAT^ATC
EcoT14I       902  CCTTGG               C^C[AT][AT]GG
FseI         1238  GGCCGGCC             GGCCGG^CC
FspI          308  TGCGCA               TGC^GCA
HindIII      1039  AAGCTT               A^AGCTT
KasI           72  GGCGCC               G^GCGCC
MflI         1050  AGATCT               [GA]^GATC[CT]
MluNI         598  TGGCCA               TGG^CCA
MscI          598  TGGCCA               TGG^CCA
NarI           72  GGCGCC               GG^CGCC
NspI         1157  ACATGT               [GA]CATG^[CT]
NspV         1492  TTCGAA               TT^CGAA
PflFI         438  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          887  GAGTC                GAGTC(4/5)
PstI          121  CTGCAG               CTGCA^G
RcaI          143  TCATGA               T^CATGA
RsrII         164  CGGTCCG              CG^G[AT]CCG
SacI         1357  GAGCTC               GAGCT^C
SapI          156  GCTCTTC              GCTCTTC(1/4)
SexAI         663  ACCTGGT              A^CC[AT]GGT
SfcI          121  CTGCAG               C^T[GA][CT]AG
SfiI          129  GGCCCAGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           72  GGCGCC               GGC^GCC
SfuI         1492  TTCGAA               TT^CGAA
SgrAI         324  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI         1333  CTCAAG               C^T[CT][GA]AG
StuI          899  AGGCCT               AGG^CCT
StyI          902  CCTTGG               C^C[AT][AT]GG
TfiI          541  GATTC                G^A[AT]TC
Tth111I       438  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          188  CCATGATGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1050  AGATCT               [GA]^GATC[CT]
XmnI           87  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         396  CGCG                 CG^CG
              861  CGCG                
AcyI           72  GGCGCC               G[GA]^CG[CT]C
              418  GACGCC              
AflIII        926  ACACGT               A^C[GA][CT]GT
             1157  ACATGT              
AlwI          498  GATCC                GGATC(4/5)
             1479  GGATC               
AspHI          97  GTGCAC               G[AT]GC[AT]^C
             1357  GAGCTC              
BanII         231  GGGCTC               G[GA]GC[CT]^C
             1357  GAGCTC              
BglI           75  GCCCCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              130  GCCCAGGTGGC         
BsaHI          72  GGCGCC               G[GA]^CG[CT]C
              418  GACGCC              
BsiHKAI        97  GTGCAC               G[AT]GC[AT]^C
             1357  GAGCTC              
BsmBI         970  CGTCTC               CGTCTC(1/5)
              573  GAGACG              
BsmFI         525  GTCCC                GGGAC(10/14)
             1132  GTCCC               
Bsp1407I      631  TGTACA               T^GTACA
              827  TGTACA              
BsrGI         631  TGTACA               T^GTACA
              827  TGTACA              
BsrI          723  ACTGG                ACTGG(1/-1)
              501  CCAGT               
BssSI         100  CACGAG               CACGAG(-5/-1)
             1009  CTCGTG              
BstAPI         99  GCACGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              360  GCAGGCGCTGC         
BstUI         396  CGCG                 CG^CG
              861  CGCG                
DrdI          962  GACAGCTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACGGCAGCGTC        
EarI          157  CTCTTC               CTCTTC(1/4)
              849  GAAGAG              
EcoNI         120  CCTGCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              291  CCTGCTGAAGG         
Hin1I          72  GGCGCC               G[GA]^CG[CT]C
              418  GACGCC              
HinfI         541  GATTC                G^A[ACGT]TC
              887  GAGTC               
Ksp632I       157  CTCTTC               CTCTTC(1/4)
              849  GAAGAG              
MvnI          396  CGCG                 CG^CG
              861  CGCG                
PpuMI         640  AGGACCT              [GA]G^G[AT]CC[CT]
              750  AGGACCT             
PvuII         943  CAGCTG               CAG^CTG
             1264  CAGCTG              
SmaI           23  CCCGGG               CCC^GGG
               78  CCCGGG              
SspBI         631  TGTACA               T^GTACA
              827  TGTACA              
Tsp509I       651  AATT                 ^AATT
              998  AATT                
TspRI         149  CACTG                CA[GC]TG[ACGT][ACGT]^
             1166  CACTG               
XmaCI          23  CCCGGG               C^CCGGG
               78  CCCGGG              
XmaI           23  CCCGGG               C^CCGGG
               78  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaI        
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BsrFI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        Tsp45I      
TthHB8I     Van91I      
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