Restriction map for pF1KB7248
 (Linear) (Six-base) MAPSORT of: KIBB7248.seq  Check: 8941  from: 1  to: 1602



KIBB7248.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1524    1602 
   Size:       1524      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316    1602 
   Size:        316    1286
 
AfeI AGC'GCT

Cuts at:      0     761    1602 
   Size:        761     841
 
AflIII A'CryG_T

Cuts at:      0     926    1157    1602 
   Size:        926     231     445

  Fragments arranged by size: 

                 926     445     231
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     799     831    1602 
   Size:        799      32     771

  Fragments arranged by size: 

                 799     771      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366     644    1570    1602 
   Size:        366     278     926      32

  Fragments arranged by size: 

                 926     366     278      32
 
ApaLI G'TGCA_C

Cuts at:      0      97    1602 
   Size:         97    1505
 
ApoI r'AATT_y

Cuts at:      0     997    1602 
   Size:        997     605
 
AvaI C'yCGr_G

Cuts at:      0      23      78     250    1021    1602 
   Size:         23      55     172     771     581

  Fragments arranged by size: 

                 771     581     172      55      23
 
BanI G'GyrC_C

Cuts at:      0      72    1602 
   Size:         72    1530
 
BanII G_rGCy'C

Cuts at:      0     235    1361    1602 
   Size:        235    1126     241

  Fragments arranged by size: 

                1126     241     235
 
BbeI G_GCGC'C

Cuts at:      0      76    1602 
   Size:         76    1526
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1160    1602 
   Size:       1160     442
 
BbvCI CC'TCA_GC

Cuts at:      0     112    1602 
   Size:        112    1490
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1483    1517    1602 
   Size:       1483      34      85

  Fragments arranged by size: 

                1483      85      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      81     136    1602 
   Size:         81      55    1466

  Fragments arranged by size: 

                1466      81      55
 
BglII A'GATC_T

Cuts at:      0    1050    1602 
   Size:       1050     552
 
Bme1580I G_kGCm'C

Cuts at:      0     101    1602 
   Size:        101    1501
 
BmgBI CAC'GTC

Cuts at:      0     743    1602 
   Size:        743     859
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     701     776    1602 
   Size:         24     677      75     826

  Fragments arranged by size: 

                 826     677      75      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     112    1602 
   Size:        112    1490
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1318    1602 
   Size:       1318     284
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1501    1602 
   Size:       1501     101
 
BsaHI Gr'CG_yC

Cuts at:      0      73     419    1602 
   Size:         73     346    1183

  Fragments arranged by size: 

                1183     346      73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     674     704    1602 
   Size:        674      30     898

  Fragments arranged by size: 

                 898     674      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     856     859    1103    1411    1602 
   Size:        856       3     244     308     191

  Fragments arranged by size: 

                 856     308     244     191       3
 
BseYI C'CCAG_C

Cuts at:      0    1570    1602 
   Size:       1570      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1523    1602 
   Size:       1523      79
 
BsiEI CG_ry'CG

Cuts at:      0    1240    1602 
   Size:       1240     362
 
BsiHKAI G_wGCw'C

Cuts at:      0     101    1361    1602 
   Size:        101    1260     241

  Fragments arranged by size: 

                1260     241     101
 
BsmI GAATG_Cn'

Cuts at:      0     670    1602 
   Size:        670     932
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     567     976    1602 
   Size:        567     409     626

  Fragments arranged by size: 

                 626     567     409
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     235    1361    1602 
   Size:        101     134    1126     241

  Fragments arranged by size: 

                1126     241     134     101
 
BspHI T'CATG_A

Cuts at:      0     143    1602 
   Size:        143    1459
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     652    1208    1524    1602 
   Size:        652     556     316      78

  Fragments arranged by size: 

                 652     556     316      78
 
BsrBI CCG'CTC

Cuts at:      0    1181    1602 
   Size:       1181     421
 
BsrFI r'CCGG_y

Cuts at:      0     325    1235    1239    1602 
   Size:        325     910       4     363

  Fragments arranged by size: 

                 910     363     325       4
 
BsrGI T'GTAC_A

Cuts at:      0     631     827    1602 
   Size:        631     196     775

  Fragments arranged by size: 

                 775     631     196
 
BssSI C'ACGA_G

Cuts at:      0     100    1009    1602 
   Size:        100     909     593

  Fragments arranged by size: 

                 909     593     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105     366    1602 
   Size:        105     261    1236

  Fragments arranged by size: 

                1236     261     105
 
BstBI TT'CG_AA

Cuts at:      0    1493    1602 
   Size:       1493     109
 
BstEII G'GTnAC_C

Cuts at:      0    1315    1602 
   Size:       1315     287
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1266    1602 
   Size:       1266     336
 
BstYI r'GATC_y

Cuts at:      0    1050    1602 
   Size:       1050     552
 
DraIII CAC_nnn'GTG

Cuts at:      0     317     342     785    1602 
   Size:        317      25     443     817

  Fragments arranged by size: 

                 817     443     317      25
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     968    1303    1602 
   Size:        968     335     299

  Fragments arranged by size: 

                 968     335     299
 
EaeI y'GGCC_r

Cuts at:      0     344     380     598     893    1237    1602 
   Size:        344      36     218     295     344     365

  Fragments arranged by size: 

                 365     344     344     295     218      36
 
EagI C'GGCC_G

Cuts at:      0    1237    1602 
   Size:       1237     365
 
EarI CTCTTCn'nnn_

Cuts at:      0     163     844    1602 
   Size:        163     681     758

  Fragments arranged by size: 

                 758     681     163
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99     970    1590    1602 
   Size:         99     871     620      12

  Fragments arranged by size: 

                 871     620      99      12
 
EcoICRI GAG'CTC

Cuts at:      0    1359    1602 
   Size:       1359     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     316     701     776    1602 
   Size:         24     292     385      75     826

  Fragments arranged by size: 

                 826     385     292      75      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     124     295    1602 
   Size:        124     171    1307

  Fragments arranged by size: 

                1307     171     124
 
EcoO109I rG'GnC_Cy

Cuts at:      0     641     751    1017    1602 
   Size:        641     110     266     585

  Fragments arranged by size: 

                 641     585     266     110
 
EcoRV GAT'ATC

Cuts at:      0     712    1602 
   Size:        712     890
 
FseI GG_CCGG'CC

Cuts at:      0    1243    1602 
   Size:       1243     359
 
FspI TGC'GCA

Cuts at:      0     310    1602 
   Size:        310    1292
 
FspAI rTGC'GCAy

Cuts at:      0     310    1602 
   Size:        310    1292
 
HaeII r_GCGC'y

Cuts at:      0      76     119     367     688     763    1602 
   Size:         76      43     248     321      75     839

  Fragments arranged by size: 

                 839     321     248      76      75      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     674     706    1048    1080    1602 
   Size:        674      32     342      32     522

  Fragments arranged by size: 

                 674     522     342      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1039    1602 
   Size:       1039     563
 
KasI G'GCGC_C

Cuts at:      0      72    1602 
   Size:         72    1530
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    1602 
   Size:        611     991
 
MscI TGG'CCA

Cuts at:      0     600    1602 
   Size:        600    1002
 
MslI CAynn'nnrTG

Cuts at:      0     427     439     454     741    1602 
   Size:        427      12      15     287     861

  Fragments arranged by size: 

                 861     427     287      15      12
 
MspA1I CmG'CkG

Cuts at:      0     184     945     979    1187    1266    1602 
   Size:        184     761      34     208      79     336

  Fragments arranged by size: 

                 761     336     208     184      79      34
 
NaeI GCC'GGC

Cuts at:      0     327    1237    1241    1602 
   Size:        327     910       4     361

  Fragments arranged by size: 

                 910     361     327       4
 
NarI GG'CG_CC

Cuts at:      0      73    1602 
   Size:         73    1529
 
NgoMIV G'CCGG_C

Cuts at:      0     325    1235    1239    1602 
   Size:        325     910       4     363

  Fragments arranged by size: 

                 910     363     325       4
 
NspI r_CATG'y

Cuts at:      0    1161    1602 
   Size:       1161     441
 
PasI CC'CwG_GG

Cuts at:      0     228    1344    1602 
   Size:        228    1116     258

  Fragments arranged by size: 

                1116     258     228
 
PciI A'CATG_T

Cuts at:      0    1157    1602 
   Size:       1157     445
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     342     699     737    1602 
   Size:        342     357      38     865

  Fragments arranged by size: 

                 865     357     342      38
 
PpuMI rG'GwC_Cy

Cuts at:      0     641     751    1602 
   Size:        641     110     851

  Fragments arranged by size: 

                 851     641     110
 
PstI C_TGCA'G

Cuts at:      0     125    1602 
   Size:        125    1477
 
PvuII CAG'CTG

Cuts at:      0     945    1266    1602 
   Size:        945     321     336

  Fragments arranged by size: 

                 945     336     321
 
RsrII CG'GwC_CG

Cuts at:      0     165    1602 
   Size:        165    1437
 
SacI G_AGCT'C

Cuts at:      0    1361    1602 
   Size:       1361     241
 
SapI GCTCTTCn'nnn_

Cuts at:      0     163    1602 
   Size:        163    1439
 
SexAI A'CCwGG_T

Cuts at:      0     663    1602 
   Size:        663     939
 
SfcI C'TryA_G

Cuts at:      0     121    1602 
   Size:        121    1481
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     136    1602 
   Size:        136    1466
 
SfoI GGC'GCC

Cuts at:      0      74    1602 
   Size:         74    1528
 
SgrAI Cr'CCGG_yG

Cuts at:      0     325    1602 
   Size:        325    1277
 
SmaI CCC'GGG

Cuts at:      0      25      80    1602 
   Size:         25      55    1522

  Fragments arranged by size: 

                1522      55      25
 
SmlI C'TyrA_G

Cuts at:      0    1333    1602 
   Size:       1333     269
 
StuI AGG'CCT

Cuts at:      0     901    1602 
   Size:        901     701
 
StyI C'CwwG_G

Cuts at:      0     902    1602 
   Size:        902     700
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     895    1384    1602 
   Size:        895     489     218

  Fragments arranged by size: 

                 895     489     218
 
TatI w'GTAC_w

Cuts at:      0     631     827    1602 
   Size:        631     196     775

  Fragments arranged by size: 

                 775     631     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     757     789    1602 
   Size:        757      32     813

  Fragments arranged by size: 

                 813     757      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     441    1602 
   Size:        441    1161
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195    1602 
   Size:        195    1407
 
XmaI C'CCGG_G

Cuts at:      0      23      78    1602 
   Size:         23      55    1524

  Fragments arranged by size: 

                1524      55      23
 
XmnI GAAnn'nnTTC

Cuts at:      0      91    1602 
   Size:         91    1511
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AloI    AlwNI    ApaLI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
    BglII Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI
    PflMI    PpuMI     PstI    PvuII    RsrII     SacI     SapI    SexAI
     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BfrBI     BlpI     BmrI     BmtI     BplI    BsaAI
    BsaBI    BsaWI    BsiWI    BspEI    BsrDI   BssHII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI    EcoRI     FalI
   HincII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    SacII     SalI
    SanDI     SbfI     ScaI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI     ZraI
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