Restriction map for pF1KB7247
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AvrII       BbvCI       BcgI        
BciVI       BclI        BfrI        BlnI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        Eam1105I    
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HpaI        
KpnI        MfeI        MluI        MseI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SbfI        SexAI       SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tru9I       Tsp509I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         1774  GTCTAC               GT^[AC][GT]AC
AccIII        857  TCCGGA               T^CCGGA
AflIII        359  ACGTGT               A^C[GA][CT]GT
AlwNI        1510  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          472  GGGCCC               GGGCC^C
Asp700I      1392  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          27  TGCGCA               TGC^GCA
BamHI         365  GGATCC               G^GATCC
BbrPI         952  CACGTG               CAC^GTG
BglII        1586  AGATCT               A^GATCT
BsaAI         952  CACGTG               [CT]AC^GT[GA]
BsaBI        1278  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         857  TCCGGA               [AT]^CCGG[AT]
BseAI         857  TCCGGA               T^CCGGA
BsiEI         173  CGGCCG               CG[GA][CT]^CG
BspEI         857  TCCGGA               T^CCGGA
DsaI            6  CCGCGG               C^C[GA][CT]GG
EagI          173  CGGCCG               C^GGCCG
EclXI         173  CGGCCG               C^GGCCG
Eco52I        173  CGGCCG               C^GGCCG
EcoNI        1764  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI           27  TGCGCA               TGC^GCA
HincII       1711  GTCAAC               GT[CT]^[GA]AC
HindII       1711  GTCAAC               GT[CT]^[GA]AC
HindIII       851  AAGCTT               A^AGCTT
KspI            6  CCGCGG               CCGC^GG
MamI         1278  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          857  TCCGGA               T^CCGGA
NotI          172  GCGGCCGC             GC^GGCCGC
NspI         1745  GCATGC               [GA]CATG^[CT]
PmaCI         952  CACGTG               CAC^GTG
PmlI          952  CACGTG               CAC^GTG
PpuMI         386  AGGTCCT              [GA]G^G[AT]CC[CT]
SacII           6  CCGCGG               CCGC^GG
SgrAI        1417  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         1579  CTCAAG               C^T[CT][GA]AG
SphI         1745  GCATGC               GCATG^C
StuI         1761  AGGCCT               AGG^CCT
XmnI         1392  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          897  GTAC                 GT^AC
             1110  GTAC                
AspI          583  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1213  GACACGGTC           
BbeI          307  GGCGCC               GGCGC^C
             1421  GGCGCC              
BpmI         1195  CTGGAG               CTGGAG(16/14)
             1498  CTGGAG              
BsaI          811  GAGACC               GGTCTC(1/5)
               63  GGTCTC              
BseRI         163  GAGGAG               GAGGAG(10/8)
             1198  GAGGAG              
BsmFI         236  GGGAC                GGGAC(10/14)
               33  GTCCC               
BsrDI        1098  CATTGC               GCAATG(2/0)
              806  GCAATG              
BsrFI         239  ACCGGC               [GA]^CCGG[CT]
             1418  ACCGGC              
BstAPI        515  GCACCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1326  GCATGAGCTGC         
BstXI         249  CCAGAAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1154  CCATGAGTCTGG        
Cfr10I        239  ACCGGC               [GA]^CCGG[CT]
             1418  ACCGGC              
HinfI         748  GACTC                G^A[ACGT]TC
             1158  GAGTC               
KasI          307  GGCGCC               G^GCGCC
             1421  GGCGCC              
NarI          307  GGCGCC               GG^CGCC
             1421  GGCGCC              
PflFI         583  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1213  GACACGGTC           
PleI          748  GACTC                GAGTC(4/5)
             1158  GAGTC               
PvuII         510  CAGCTG               CAG^CTG
              577  CAGCTG              
RsaI          897  GTAC                 GT^AC
             1110  GTAC                
SapI          501  GCTCTTC              GCTCTTC(1/4)
             1068  GCTCTTC             
ScaI          896  AGTACT               AGT^ACT
             1109  AGTACT              
SfiI         1305  GGCCATTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1524  GGCCTCACTGGCC       
SfoI          307  GGCGCC               GGC^GCC
             1421  GGCGCC              
Tth111I       583  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1213  GACACGGTC           
XcmI         1151  CCACCATGAGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1184  CCATACACGGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaHI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       
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