Restriction map for pF1KB7247
 (Linear) (Six-base) MAPSORT of: KIBB7247.seq  Check: 9436  from: 1  to: 1788



KIBB7247.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1007    1039    1788 
   Size:       1007      32     749

  Fragments arranged by size: 

                1007     749      32
 
AccI GT'mk_AC

Cuts at:      0    1775    1788 
   Size:       1775      13
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     403     870    1788 
   Size:        140     263     467     918

  Fragments arranged by size: 

                 918     467     263     140
 
AflIII A'CryG_T

Cuts at:      0     359    1788 
   Size:        359    1429
 
AleI CACnn'nnGTG

Cuts at:      0     968    1788 
   Size:        968     820
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1071    1788 
   Size:       1071     717
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1515    1788 
   Size:       1515     273
 
ApaI G_GGCC'C

Cuts at:      0     476    1788 
   Size:        476    1312
 
AvaI C'yCGr_G

Cuts at:      0      14     270    1444    1788 
   Size:         14     256    1174     344

  Fragments arranged by size: 

                1174     344     256      14
 
BamHI G'GATC_C

Cuts at:      0     365    1788 
   Size:        365    1423
 
BanI G'GyrC_C

Cuts at:      0     307     957     970    1421    1788 
   Size:        307     650      13     451     367

  Fragments arranged by size: 

                 650     451     367     307      13
 
BanII G_rGCy'C

Cuts at:      0      15     278     476    1788 
   Size:         15     263     198    1312

  Fragments arranged by size: 

                1312     263     198      15
 
BbeI G_GCGC'C

Cuts at:      0     311    1425    1788 
   Size:        311    1114     363

  Fragments arranged by size: 

                1114     363     311
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     582     603    1728    1788 
   Size:        582      21    1125      60

  Fragments arranged by size: 

                1125     582      60      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0      19     253    1175    1280    1312    1531    1788 
   Size:         19     234     922     105      32     219     257

  Fragments arranged by size: 

                 922     257     234     219     105      32      19
 
BglII A'GATC_T

Cuts at:      0    1586    1788 
   Size:       1586     202
 
BlpI GC'TnA_GC

Cuts at:      0      87     773    1472    1788 
   Size:         87     686     699     316

  Fragments arranged by size: 

                 699     686     316      87
 
Bme1580I G_kGCm'C

Cuts at:      0     476     975    1180    1578    1788 
   Size:        476     499     205     398     210

  Fragments arranged by size: 

                 499     476     398     210     205
 
BmgBI CAC'GTC

Cuts at:      0     360    1788 
   Size:        360    1428
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1279    1788 
   Size:       1279     509
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1216    1519    1788 
   Size:       1216     303     269

  Fragments arranged by size: 

                1216     303     269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1564    1788 
   Size:       1564     224
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      69     805    1788 
   Size:         69     736     983

  Fragments arranged by size: 

                 983     736      69
 
BsaAI yAC'GTr

Cuts at:      0     954    1788 
   Size:        954     834
 
BsaBI GATnn'nnATC

Cuts at:      0    1282    1788 
   Size:       1282     506
 
BsaHI Gr'CG_yC

Cuts at:      0      49     308     620    1422    1788 
   Size:         49     259     312     802     366

  Fragments arranged by size: 

                 802     366     312     259      49
 
BsaWI w'CCGG_w

Cuts at:      0     857    1788 
   Size:        857     931
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1528    1558    1751    1781    1788 
   Size:       1528      30     193      30       7

  Fragments arranged by size: 

                1528     193      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178    1213    1788 
   Size:        178    1035     575

  Fragments arranged by size: 

                1035     575     178
 
BseYI C'CCAG_C

Cuts at:      0      38    1026    1503    1788 
   Size:         38     988     477     285

  Fragments arranged by size: 

                 988     477     285      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498     985    1250    1399    1788 
   Size:        498     487     265     149     389

  Fragments arranged by size: 

                 498     487     389     265     149
 
BsiEI CG_ry'CG

Cuts at:      0     176    1788 
   Size:        176    1612
 
BsiHKAI G_wGCw'C

Cuts at:      0     452     503     638    1070    1788 
   Size:        452      51     135     432     718

  Fragments arranged by size: 

                 718     452     432     135      51
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     278     452     476     503     638     975    1070 
   Size:         15     263     174      24      27     135     337      95

Cuts at:   1070    1180    1578    1788 
   Size:        110     398     210

  Fragments arranged by size: 

                 398     337     263     210     174     135     110      95
                  27      24      15
 
BspEI T'CCGG_A

Cuts at:      0     857    1788 
   Size:        857     931
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     725    1007    1039    1788 
   Size:        725     282      32     749

  Fragments arranged by size: 

                 749     725     282      32
 
BsrBI CCG'CTC

Cuts at:      0      19      86     172    1788 
   Size:         19      67      86    1616

  Fragments arranged by size: 

                1616      86      67      19
 
BsrDI GCAATG_nn'

Cuts at:      0     813    1097    1788 
   Size:        813     284     691

  Fragments arranged by size: 

                 813     691     284
 
BsrFI r'CCGG_y

Cuts at:      0     239    1418    1788 
   Size:        239    1179     370

  Fragments arranged by size: 

                1179     370     239
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     521    1332    1788 
   Size:        521     811     456

  Fragments arranged by size: 

                 811     521     456
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     256    1161    1788 
   Size:        256     905     627

  Fragments arranged by size: 

                 905     627     256
 
BstYI r'GATC_y

Cuts at:      0     365     459    1586    1788 
   Size:        365      94    1127     202

  Fragments arranged by size: 

                1127     365     202      94
 
BtgI C'CryG_G

Cuts at:      0       6    1788 
   Size:          6    1782
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1269    1368    1788 
   Size:       1269      99     420

  Fragments arranged by size: 

                1269     420      99
 
BtsI GCAGTG_nn'

Cuts at:      0     645    1788 
   Size:        645    1143
 
DraIII CAC_nnn'GTG

Cuts at:      0     447     686     845    1622    1755    1788 
   Size:        447     239     159     777     133      33

  Fragments arranged by size: 

                 777     447     239     159     133      33
 
EaeI y'GGCC_r

Cuts at:      0     173     341     374     707     734    1304    1313    1788 
   Size:        173     168      33     333      27     570       9     475

  Fragments arranged by size: 

                 570     475     333     173     168      33      27       9
            
 
EagI C'GGCC_G

Cuts at:      0     173    1788 
   Size:        173    1615
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     508    1075    1402    1600    1788 
   Size:        271     237     567     327     198     188

  Fragments arranged by size: 

                 567     327     271     237     198     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     403     870    1216    1519    1788 
   Size:        140     263     467     346     303     269

  Fragments arranged by size: 

                 467     346     303     269     263     140
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1768    1788 
   Size:       1768      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387     472     473    1410    1449    1512    1788 
   Size:        387      85       1     937      39      63     276

  Fragments arranged by size: 

                 937     387     276      85      63      39       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     793    1788 
   Size:        793     995
 
FspI TGC'GCA

Cuts at:      0      29    1788 
   Size:         29    1759
 
HaeII r_GCGC'y

Cuts at:      0      94     311    1323    1425    1788 
   Size:         94     217    1012     102     363

  Fragments arranged by size: 

                1012     363     217     102      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97    1788 
   Size:         65      32    1691

  Fragments arranged by size: 

                1691      65      32
 
HincII GTy'rAC

Cuts at:      0    1713    1788 
   Size:       1713      75
 
HindIII A'AGCT_T

Cuts at:      0     851    1788 
   Size:        851     937
 
KasI G'GCGC_C

Cuts at:      0     307    1421    1788 
   Size:        307    1114     367

  Fragments arranged by size: 

                1114     367     307
 
MscI TGG'CCA

Cuts at:      0     376     709    1306    1315    1788 
   Size:        376     333     597       9     473

  Fragments arranged by size: 

                 597     473     376     333       9
 
MslI CAynn'nnrTG

Cuts at:      0     604     787     968    1788 
   Size:        604     183     181     820

  Fragments arranged by size: 

                 820     604     183     181
 
MspA1I CmG'CkG

Cuts at:      0       8      38     512     566     579    1023    1521    1788 
   Size:          8      30     474      54      13     444     498     267

  Fragments arranged by size: 

                 498     474     444     267      54      30      13       8
            
 
NarI GG'CG_CC

Cuts at:      0     308    1422    1788 
   Size:        308    1114     366

  Fragments arranged by size: 

                1114     366     308
 
NotI GC'GGCC_GC

Cuts at:      0     173    1788 
   Size:        173    1615
 
NspI r_CATG'y

Cuts at:      0    1749    1788 
   Size:       1749      39
 
PmlI CAC'GTG

Cuts at:      0     954    1788 
   Size:        954     834
 
PpuMI rG'GwC_Cy

Cuts at:      0     387    1788 
   Size:        387    1401
 
PspOMI G'GGCC_C

Cuts at:      0     472    1788 
   Size:        472    1316
 
PstI C_TGCA'G

Cuts at:      0     322    1379    1520    1788 
   Size:        322    1057     141     268

  Fragments arranged by size: 

                1057     322     268     141
 
PvuII CAG'CTG

Cuts at:      0     512     579    1788 
   Size:        512      67    1209

  Fragments arranged by size: 

                1209     512      67
 
SacII CC_GC'GG

Cuts at:      0       9    1788 
   Size:          9    1779
 
SapI GCTCTTCn'nnn_

Cuts at:      0     508    1075    1788 
   Size:        508     567     713

  Fragments arranged by size: 

                 713     567     508
 
ScaI AGT'ACT

Cuts at:      0     898    1111    1788 
   Size:        898     213     677

  Fragments arranged by size: 

                 898     677     213
 
SfcI C'TryA_G

Cuts at:      0     318    1375    1516    1548    1788 
   Size:        318    1057     141      32     240

  Fragments arranged by size: 

                1057     318     240     141      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1312    1531    1788 
   Size:       1312     219     257

  Fragments arranged by size: 

                1312     257     219
 
SfoI GGC'GCC

Cuts at:      0     309    1423    1788 
   Size:        309    1114     365

  Fragments arranged by size: 

                1114     365     309
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1418    1788 
   Size:       1418     370
 
SmlI C'TyrA_G

Cuts at:      0    1579    1788 
   Size:       1579     209
 
SphI G_CATG'C

Cuts at:      0    1749    1788 
   Size:       1749      39
 
StuI AGG'CCT

Cuts at:      0    1763    1788 
   Size:       1763      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      51     292     343     471     621     958    1249    1788 
   Size:         51     241      51     128     150     337     291     539

  Fragments arranged by size: 

                 539     337     291     241     150     128      51      51
            
 
TatI w'GTAC_w

Cuts at:      0     896    1109    1788 
   Size:        896     213     679

  Fragments arranged by size: 

                 896     679     213
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1751    1783    1788 
   Size:       1751      32       5

  Fragments arranged by size: 

                1751      32       5
 
Tth111I GACn'n_nGTC

Cuts at:      0     586    1216    1788 
   Size:        586     630     572

  Fragments arranged by size: 

                 630     586     572
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1158    1191    1788 
   Size:       1158      33     597

  Fragments arranged by size: 

                1158     597      33
 
XmnI GAAnn'nnTTC

Cuts at:      0    1396    1788 
   Size:       1396     392
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI
     EarI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     MscI     MslI   MspA1I     NarI     NotI
     NspI     PmlI    PpuMI   PspOMI     PstI    PvuII    SacII     SapI
     ScaI     SfcI     SfiI     SfoI    SgrAI     SmlI     SphI     StuI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BmtI     BplI   Bpu10I    BsiWI
     BsmI    BsmBI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI
    EcoRI    EcoRV     FseI    FspAI     HpaI     KpnI     MfeI     MluI
     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SbfI    SexAI     SgfI     SmaI    SnaBI     SpeI     SrfI     SspI
     StyI     SwaI     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com