Restriction map for pF1KB7246
 (Linear) (Six-base) MAPSORT of: KIBB7246.seq  Check: 2578  from: 1  to: 1560



KIBB7246.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18      72      90     307     816     916    1537    1560 
   Size:         18      54      18     217     509     100     621      23

  Fragments arranged by size: 

                 621     509     217     100      54      23      18      18
            
 
AfeI AGC'GCT

Cuts at:      0      39    1320    1491    1560 
   Size:         39    1281     171      69

  Fragments arranged by size: 

                1281     171      69      39
 
AflIII A'CryG_T

Cuts at:      0     788    1560 
   Size:        788     772
 
AleI CACnn'nnGTG

Cuts at:      0     620     793    1560 
   Size:        620     173     767

  Fragments arranged by size: 

                 767     620     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273     471    1014    1164    1194    1560 
   Size:        273     198     543     150      30     366

  Fragments arranged by size: 

                 543     366     273     198     150      30
 
ApaI G_GGCC'C

Cuts at:      0      24    1560 
   Size:         24    1536
 
ApaLI G'TGCA_C

Cuts at:      0    1281    1560 
   Size:       1281     279
 
BanI G'GyrC_C

Cuts at:      0     137    1560 
   Size:        137    1423
 
BanII G_rGCy'C

Cuts at:      0      24     224     546    1444    1560 
   Size:         24     200     322     898     116

  Fragments arranged by size: 

                 898     322     200     116      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1528    1549    1560 
   Size:       1528      21      11

  Fragments arranged by size: 

                1528      21      11
 
BbvCI CC'TCA_GC

Cuts at:      0     893     899    1560 
   Size:        893       6     661

  Fragments arranged by size: 

                 893     661       6
 
BglI GCCn_nnn'nGGC

Cuts at:      0     146     467    1560 
   Size:        146     321    1093

  Fragments arranged by size: 

                1093     321     146
 
BglII A'GATC_T

Cuts at:      0     983    1560 
   Size:        983     577
 
BlpI GC'TnA_GC

Cuts at:      0      45    1079    1560 
   Size:         45    1034     481

  Fragments arranged by size: 

                1034     481      45
 
Bme1580I G_kGCm'C

Cuts at:      0      24     235    1285    1560 
   Size:         24     211    1050     275

  Fragments arranged by size: 

                1050     275     211      24
 
BmgBI CAC'GTC

Cuts at:      0     791    1560 
   Size:        791     769
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     300    1560 
   Size:        300    1260
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     742    1560 
   Size:        742     818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     436     493     511     538     727     754    1153 
   Size:        415      21      57      18      27     189      27     399

Cuts at:   1153    1270    1352    1439    1470    1482    1560 
   Size:        117      82      87      31      12      78

  Fragments arranged by size: 

                 415     399     189     117      87      82      78      57
                  31      27      27      21      18      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     505     679     893     899    1112    1560 
   Size:        505     174     214       6     213     448

  Fragments arranged by size: 

                 505     448     214     213     174       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     637     652    1021    1087    1560 
   Size:         48     589      15     369      66     473

  Fragments arranged by size: 

                 589     473     369      66      48      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325    1560 
   Size:        325    1235
 
BsaBI GATnn'nnATC

Cuts at:      0     295    1560 
   Size:        295    1265
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     409     439     571     577     601     607    1347    1377 
   Size:        409      30     132       6      24       6     740      30

Cuts at:   1377    1560 
   Size:        183

  Fragments arranged by size: 

                 740     409     183     132      30      30      24       6
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     595     598     619     622     883    1273    1293    1349 
   Size:        595       3      21       3     261     390      20      56

Cuts at:   1349    1560 
   Size:        211

  Fragments arranged by size: 

                 595     390     261     211      56      21      20       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     183     759     944    1560 
   Size:        183     576     185     616

  Fragments arranged by size: 

                 616     576     185     183
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1284    1386    1560 
   Size:       1284     102     174

  Fragments arranged by size: 

                1284     174     102
 
BsiHKAI G_wGCw'C

Cuts at:      0    1204    1285    1360    1560 
   Size:       1204      81      75     200

  Fragments arranged by size: 

                1204     200      81      75
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     412    1560 
   Size:        412    1148
 
Bsp1286I G_dGCh'C

Cuts at:      0      24     224     235     546    1204    1285    1360    1444 
   Size:         24     200      11     311     658      81      75      84

Cuts at:   1444    1560 
   Size:        116

  Fragments arranged by size: 

                 658     311     200     116      84      81      75      24
                  11
 
BssSI C'ACGA_G

Cuts at:      0     152    1560 
   Size:        152    1408
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435    1560 
   Size:        435    1125
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     952    1560 
   Size:        952     608
 
BstYI r'GATC_y

Cuts at:      0     983    1560 
   Size:        983     577
 
BtgI C'CryG_G

Cuts at:      0     806    1560 
   Size:        806     754
 
ClaI AT'CG_AT

Cuts at:      0     299    1560 
   Size:        299    1261
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     646    1530    1560 
   Size:        646     884      30

  Fragments arranged by size: 

                 884     646      30
 
EaeI y'GGCC_r

Cuts at:      0    1151    1560 
   Size:       1151     409
 
EarI CTCTTCn'nnn_

Cuts at:      0     749    1560 
   Size:        749     811
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18      72      90     307     415     436     493     511 
   Size:         18      54      18     217     108      21      57      18

Cuts at:    511     538     727     754     816     916    1153    1270    1352 
   Size:         27     189      27      62     100     237     117      82

Cuts at:   1352    1439    1470    1482    1537    1560 
   Size:         87      31      12      55      23

  Fragments arranged by size: 

                 237     217     189     117     108     100      87      82
                  62      57      55      54      31      27      27      23
                  21      18      18      18      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     655    1560 
   Size:        655     905
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411     468    1394    1452    1560 
   Size:        411      57     926      58     108

  Fragments arranged by size: 

                 926     411     108      58      57
 
HaeII r_GCGC'y

Cuts at:      0      41    1322    1493    1560 
   Size:         41    1281     171      67

  Fragments arranged by size: 

                1281     171      67      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    1456    1488    1560 
   Size:        577      32     847      32      72

  Fragments arranged by size: 

                 847     577      72      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1207    1560 
   Size:       1207     353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     674    1451    1560 
   Size:        674     777     109

  Fragments arranged by size: 

                 777     674     109
 
MscI TGG'CCA

Cuts at:      0    1153    1560 
   Size:       1153     407
 
MslI CAynn'nnrTG

Cuts at:      0     620     746     793    1560 
   Size:        620     126      47     767

  Fragments arranged by size: 

                 767     620     126      47
 
MspA1I CmG'CkG

Cuts at:      0     516    1502    1560 
   Size:        516     986      58

  Fragments arranged by size: 

                 986     516      58
 
NcoI C'CATG_G

Cuts at:      0     806    1560 
   Size:        806     754
 
PasI CC'CwG_GG

Cuts at:      0    1390    1560 
   Size:       1390     170
 
PfoI T'CCnGG_A

Cuts at:      0     392     413    1560 
   Size:        392      21    1147

  Fragments arranged by size: 

                1147     392      21
 
PpuMI rG'GwC_Cy

Cuts at:      0     411    1394    1452    1560 
   Size:        411     983      58     108

  Fragments arranged by size: 

                 983     411     108      58
 
PspOMI G'GGCC_C

Cuts at:      0      20    1560 
   Size:         20    1540
 
PstI C_TGCA'G

Cuts at:      0     440    1019    1061    1560 
   Size:        440     579      42     499

  Fragments arranged by size: 

                 579     499     440      42
 
PvuII CAG'CTG

Cuts at:      0     516    1502    1560 
   Size:        516     986      58

  Fragments arranged by size: 

                 986     516      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0     749    1560 
   Size:        749     811
 
SbfI CC_TGCA'GG

Cuts at:      0    1061    1560 
   Size:       1061     499
 
SfcI C'TryA_G

Cuts at:      0     436    1015    1057    1560 
   Size:        436     579      42     503

  Fragments arranged by size: 

                 579     503     436      42
 
SmlI C'TyrA_G

Cuts at:      0      63     631     652    1036    1102    1560 
   Size:         63     568      21     384      66     458

  Fragments arranged by size: 

                 568     458     384      66      63      21
 
StuI AGG'CCT

Cuts at:      0     490     565     904    1560 
   Size:        490      75     339     656

  Fragments arranged by size: 

                 656     490     339      75
 
StyI C'CwwG_G

Cuts at:      0     806    1118    1560 
   Size:        806     312     442

  Fragments arranged by size: 

                 806     442     312
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     409     441    1347    1379    1560 
   Size:        409      32     906      32     181

  Fragments arranged by size: 

                 906     409     181      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1161    1434    1560 
   Size:       1161     273     126

  Fragments arranged by size: 

                1161     273     126
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI    ApaLI     BanI
    BanII     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BssSI   BstAPI
    BstXI    BstYI     BtgI     ClaI     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I  HindIII     MmeI     MscI     MslI
   MspA1I     NcoI     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII
     SapI     SbfI     SfcI     SmlI     StuI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BmtI
    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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