Restriction map for pF1KB7245
 (Linear) (Six-base) MAPSORT of: KIBB7245.seq  Check: 2218  from: 1  to: 921



KIBB7245.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     724     921 
   Size:        724     197
 
AccI GT'mk_AC

Cuts at:      0     101     921 
   Size:        101     820
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     921 
   Size:        206     715
 
AleI CACnn'nnGTG

Cuts at:      0     445     921 
   Size:        445     476
 
AlwNI CAG_nnn'CTG

Cuts at:      0     229     921 
   Size:        229     692
 
AvaI C'yCGr_G

Cuts at:      0      59     921 
   Size:         59     862
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0       8      41     921 
   Size:          8      33     880

  Fragments arranged by size: 

                 880      33       8
 
BanI G'GyrC_C

Cuts at:      0     325     921 
   Size:        325     596
 
BanII G_rGCy'C

Cuts at:      0      50      68     921 
   Size:         50      18     853

  Fragments arranged by size: 

                 853      50      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     327     921 
   Size:         30     297     594

  Fragments arranged by size: 

                 594     297      30
 
BbvCI CC'TCA_GC

Cuts at:      0     673     921 
   Size:        673     248
 
BglII A'GATC_T

Cuts at:      0     299     596     921 
   Size:        299     297     325

  Fragments arranged by size: 

                 325     299     297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     604     921 
   Size:         52     552     317

  Fragments arranged by size: 

                 552     317      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     673     921 
   Size:        673     248
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     921 
   Size:        577     344
 
BsaAI yAC'GTr

Cuts at:      0      20     921 
   Size:         20     901
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     443     473     921 
   Size:        443      30     448

  Fragments arranged by size: 

                 448     443      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     628     921 
   Size:        244     384     293

  Fragments arranged by size: 

                 384     293     244
 
BseYI C'CCAG_C

Cuts at:      0     158     921 
   Size:        158     763
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     702     921 
   Size:        549     153     219

  Fragments arranged by size: 

                 549     219     153
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     921 
   Size:         68     853
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      68     921 
   Size:         50      18     853

  Fragments arranged by size: 

                 853      50      18
 
BspHI T'CATG_A

Cuts at:      0     404     830     921 
   Size:        404     426      91

  Fragments arranged by size: 

                 426     404      91
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724     921 
   Size:        724     197
 
BsrDI GCAATG_nn'

Cuts at:      0     368     921 
   Size:        368     553
 
BsrFI r'CCGG_y

Cuts at:      0     911     921 
   Size:        911      10
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     447     519     921 
   Size:        117     330      72     402

  Fragments arranged by size: 

                 402     330     117      72
 
BstYI r'GATC_y

Cuts at:      0     299     596     921 
   Size:        299     297     325

  Fragments arranged by size: 

                 325     299     297
 
Bsu36I CC'TnA_GG

Cuts at:      0     865     921 
   Size:        865      56
 
BtgI C'CryG_G

Cuts at:      0     329     921 
   Size:        329     592
 
BtsI GCAGTG_nn'

Cuts at:      0     227     493     577     896     921 
   Size:        227     266      84     319      25

  Fragments arranged by size: 

                 319     266     227      84      25
 
EaeI y'GGCC_r

Cuts at:      0     819     921 
   Size:        819     102
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     921 
   Size:        225     696
 
EcoICRI GAG'CTC

Cuts at:      0      66     921 
   Size:         66     855
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     206     604     921 
   Size:         52     154     398     317

  Fragments arranged by size: 

                 398     317     154      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     443     475     666     698     921 
   Size:        241      32     170      32     191      32     223

  Fragments arranged by size: 

                 241     223     191     170      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     805     921 
   Size:        805     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     626     921 
   Size:        626     295
 
MslI CAynn'nnrTG

Cuts at:      0     115     142     172     403     445     664     733     829 
   Size:        115      27      30     231      42     219      69      96

Cuts at:    829     921 
   Size:         92

  Fragments arranged by size: 

                 231     219     115      96      92      69      42      30
                  27
 
MspA1I CmG'CkG

Cuts at:      0     477     921 
   Size:        477     444
 
NcoI C'CATG_G

Cuts at:      0     329     921 
   Size:        329     592
 
NspI r_CATG'y

Cuts at:      0     882     921 
   Size:        882      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     921 
   Size:        318     603
 
PmlI CAC'GTG

Cuts at:      0      20     921 
   Size:         20     901
 
PvuII CAG'CTG

Cuts at:      0     477     921 
   Size:        477     444
 
SacI G_AGCT'C

Cuts at:      0      68     921 
   Size:         68     853
 
ScaI AGT'ACT

Cuts at:      0     551     921 
   Size:        551     370
 
SmlI C'TyrA_G

Cuts at:      0     592     921 
   Size:        592     329
 
StyI C'CwwG_G

Cuts at:      0     329     921 
   Size:        329     592
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     411     435     739     921 
   Size:        186     225      24     304     182

  Fragments arranged by size: 

                 304     225     186     182      24
 
TatI w'GTAC_w

Cuts at:      0     176     549     921 
   Size:        176     373     372

  Fragments arranged by size: 

                 373     372     176
 
Tth111I GACn'n_nGTC

Cuts at:      0      34     921 
   Size:         34     887
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     243     921 
   Size:        243     678
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     AvaI     BaeI     BanI
    BanII     BbsI    BbvCI    BglII     BpmI   Bpu10I    BpuEI    BsaAI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    Hin4I  HindIII     MmeI     MslI   MspA1I
     NcoI     NspI    PflMI     PmlI    PvuII     SacI     ScaI     SmlI
     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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