Restriction map for pF1KB7243
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsiWI       BsmI        BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
ClaI        CpoI        DraI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MseI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspI        SwaI        Tru9I       
Tsp509I     XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccIII        585  TCCGGA               T^CCGGA
AcyI         1285  GGCGTC               G[GA]^CG[CT]C
AflIII        158  ACACGT               A^C[GA][CT]GT
AocI         1100  CCTCAGG              CC^T[ACGT]AGG
AspI         1012  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1295  TGGCCA               TGG^CCA
BamHI         575  GGATCC               G^GATCC
BbsI           25  GTCTTC               GAAGAC(2/6)
BbvCI         822  CCTCAGC              CCTCAGC(-5/-2)
BcgI          761  GCAGGCTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI          25  GTCTTC               GAAGAC(2/6)
BsaHI        1285  GGCGTC               G[GA]^CG[CT]C
BsaWI         585  TCCGGA               [AT]^CCGG[AT]
BseAI         585  TCCGGA               T^CCGGA
BsiEI        1247  CGGCCG               CG[GA][CT]^CG
BsmBI         948  CGTCTC               CGTCTC(1/5)
Bsp1407I     1109  TGTACA               T^GTACA
BspEI         585  TCCGGA               T^CCGGA
BspMI          21  GCAGGT               ACCTGC(4/8)
BsrGI        1109  TGTACA               T^GTACA
BstXI         356  CCACGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         575  GGATCC               [GA]^GATC[CT]
Bsu36I       1100  CCTCAGG              CC^T[ACGT]AGG
DraIII        869  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          706  GACTACAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1247  CGGCCG               C^GGCCG
EclXI        1247  CGGCCG               C^GGCCG
Eco52I       1247  CGGCCG               C^GGCCG
Eco81I       1100  CCTCAGG              CC^T[ACGT]AGG
EcoT14I      1346  CCTTGG               C^C[AT][AT]GG
HaeII         315  GGCGCT               [GA]GCGC^[CT]
Hin1I        1285  GGCGTC               G[GA]^CG[CT]C
HincII        526  GTCAAC               GT[CT]^[GA]AC
HindII        526  GTCAAC               GT[CT]^[GA]AC
MflI          575  GGATCC               [GA]^GATC[CT]
MluNI        1295  TGGCCA               TGG^CCA
MroI          585  TCCGGA               T^CCGGA
MscI         1295  TGGCCA               TGG^CCA
NaeI         1257  GCCGGC               GCC^GGC
NgoMIV       1257  GCCGGC               G^CCGGC
NspI         1220  ACATGC               [GA]CATG^[CT]
PaeR7I       1168  CTCGAG               C^TCGAG
PflFI        1012  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          865  GAGTC                GAGTC(4/5)
PpuMI         443  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         543  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          295  CTGCAG               CTGCA^G
PvuII         981  CAGCTG               CAG^CTG
SacI          481  GAGCTC               GAGCT^C
SapI         1356  GCTCTTC              GCTCTTC(1/4)
SbfI          294  CCTGCAGG             CCTGCA^GG
ScaI          554  AGTACT               AGT^ACT
SfiI          489  GGCCGGGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1168  CTCGAG               C^T[CT][GA]AG
Sse8387I      294  CCTGCAGG             CCTGCA^GG
SspBI        1109  TGTACA               T^GTACA
StyI         1346  CCTTGG               C^C[AT][AT]GG
TaqI         1169  TCGA                 T^CGA
TfiI          251  GAATC                G^A[AT]TC
Tth111I      1012  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1169  TCGA                 T^CGA
XcmI          839  CCACACTGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1168  CTCGAG               C^TCGAG
XhoII         575  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          715  GTCTAC               GT^[AC][GT]AC
             1018  GTCTAC              
ApaI          654  GGGCCC               GGGCC^C
              852  GGGCCC              
AvaI           48  CTCGGG               C^[CT]CG[GA]G
             1168  CTCGAG              
BsgI          275  CTGCAC               GTGCAG(16/14)
             1126  CTGCAC              
BsoBI          48  CTCGGG               C^[CT]CG[GA]G
             1168  CTCGAG              
BsrFI         452  GCCGGT               [GA]^CCGG[CT]
             1257  GCCGGC              
BssSI        1129  CACGAG               CACGAG(-5/-1)
              769  CTCGTG              
Cfr10I        452  GCCGGT               [GA]^CCGG[CT]
             1257  GCCGGC              
DsaI          371  CCACGG               C^C[GA][CT]GG
              461  CCGTGG              
EarI          166  CTCTTC               CTCTTC(1/4)
             1357  CTCTTC              
EcoNI          84  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1125  CCTGCACGAGG         
HinfI         251  GAATC                G^A[ACGT]TC
              865  GAGTC               
Ksp632I       166  CTCTTC               CTCTTC(1/4)
             1357  CTCTTC              
PflMI         219  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              441  CCAGGTCCTGG         
SfcI          295  CTGCAG               C^T[GA][CT]AG
              708  CTACAG              
StuI           60  AGGCCT               AGG^CCT
             1104  AGGCCT              
Van91I        219  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              441  CCAGGTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com