Restriction map for pF1KB7241
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboII       MluI        MluNI       MroI        
MscI        MslI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        TaqI        TfiI        TspRI       
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         292  GACGTC               GACGT^C
AciI          284  GCGG                 CCGC(-3/-1)
AcyI          292  GACGTC               G[GA]^CG[CT]C
AlwI          220  GGATC                GGATC(4/5)
BanI          209  GGTGCC               G^G[CT][GA]CC
BanII         427  GGGCTC               G[GA]GC[CT]^C
BmyI          427  GGGCTC               G[AGT]GC[ACT]^C
BsaHI         292  GACGTC               G[GA]^CG[CT]C
BsaI          193  GAGACC               GGTCTC(1/5)
BsaJI         197  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         357  TCCGGT               [AT]^CCGG[AT]
BsmAI         193  GAGAC                GTCTC(1/5)
BsmFI          32  GTCCC                GGGAC(10/14)
Bsp1286I      427  GGGCTC               G[AGT]GC[ACT]^C
BspHI          92  TCATGA               T^CATGA
BspMI         167  ACCTGC               ACCTGC(4/8)
BsrDI         407  GCAATG               GCAATG(2/0)
BssKI         396  CCTGG                ^CC[ACGT]GG
BstNI         396  CCTGG                CC^[AT]GG
BstYI         220  GGATCT               [GA]^GATC[CT]
CfoI          171  GCGC                 GCG^C
DpnII         221  GATC                 ^GATC
EcoRII        396  CCTGG                ^CC[AT]GG
EcoT14I       197  CCAAGG               C^C[AT][AT]GG
HaeIII          7  GGCC                 GG^CC
HapII         358  CCGG                 C^CGG
HhaI          171  GCGC                 GCG^C
Hin1I         292  GACGTC               G[GA]^CG[CT]C
HinP1I        171  GCGC                 G^CGC
HinfI          24  GAGTC                G^A[ACGT]TC
HpaII         358  CCGG                 C^CGG
MaeII         293  ACGT                 A^CGT
MaeIII        305  GTGAC                ^GT[ACGT]AC
MboI          221  GATC                 ^GATC
MfeI          129  CAATTG               C^AATTG
MflI          220  GGATCT               [GA]^GATC[CT]
MseI           79  TTAA                 T^TAA
MspI          358  CCGG                 C^CGG
MunI          129  CAATTG               C^AATTG
MvaI          396  CCTGG                CC^[AT]GG
NdeII         221  GATC                 ^GATC
PflMI          21  CCAGAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           24  GAGTC                GAGTC(4/5)
PpuMI          30  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI           11  CTGCAG               CTGCA^G
RcaI           92  TCATGA               T^CATGA
Sau3AI        221  GATC                 ^GATC
SbfI           10  CCTGCAGG             CCTGCA^GG
ScrFI         396  CCTGG                CC^[ACGT]GG
SfcI           11  CTGCAG               C^T[GA][CT]AG
Sse8387I       10  CCTGCAGG             CCTGCA^GG
StyI          197  CCAAGG               C^C[AT][AT]GG
Tru9I          79  TTAA                 T^TAA
Tsp45I        305  GTGAC                ^GT[GC]AC
Tsp509I       130  AATT                 ^AATT
Van91I         21  CCAGAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         220  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AvaII          31  GGTCC                G^G[AT]CC
               72  GGTCC               
BstAPI          4  GCAGGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              248  GCAGCAGCTGC         
Cac8I           4  GCAGGC               GC[ACGT]^[ACGT]GC
              244  GCTGGC              
DraII           6  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               30  GGGTCCC             
EcoO109I        6  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               30  GGGTCCC             
HphI          300  GGTGA                GGTGA(8/7)
              236  TCACC               
NlaIII         93  CATG                 CATG^
              297  CATG                
PvuII         242  CAGCTG               CAG^CTG
              252  CAGCTG              
SfaNI         182  GATGC                GCATC(5/9)
              257  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        AlwNI       BbvI        BsiYI       BslI        
Cfr13I      Fnu4HI      ItaI        MnlI        MspA1I      
MwoI        NlaIV       Sau96I      TseI        
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