Restriction map for
pF1KB7241
(Circular) (Six-base) MAPSORT of: pF1KB7241.seq Check: 4270 from: 1 to: 3554
pF1KB7241 3554 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 404 404
Size: 3554
AccI GT'mk_AC
Cuts at: 592 592
Size: 3554
Acc65I G'GTAC_C
Cuts at: 570 570
Size: 3554
AclI AA'CG_TT
Cuts at: 3394 3394
Size: 3554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1811 2558 2826 1379
Size: 432 747 268 2107
Fragments arranged by size:
2107 747 432 268
AflIII A'CryG_T
Cuts at: 906 2010 2987 906
Size: 1104 977 1473
Fragments arranged by size:
1473 1104 977
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 3554
AlwNI CAG_nnn'CTG
Cuts at: 118 135 362 859 2426 118
Size: 17 227 497 1567 1246
Fragments arranged by size:
1567 1246 497 227 17
ApaLI G'TGCA_C
Cuts at: 2324 2324
Size: 3554
ApoI r'AATT_y
Cuts at: 558 2907 3163 558
Size: 2349 256 949
Fragments arranged by size:
2349 949 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
AvaI C'yCGr_G
Cuts at: 574 574
Size: 3554
BamHI G'GATC_C
Cuts at: 579 579
Size: 3554
BanI G'GyrC_C
Cuts at: 317 570 984 1232 1267 317
Size: 253 414 248 35 2604
Fragments arranged by size:
2604 414 253 248 35
BanII G_rGCy'C
Cuts at: 539 568 1598 539
Size: 29 1030 2495
Fragments arranged by size:
2495 1030 29
BbeI G_GCGC'C
Cuts at: 988 1236 988
Size: 248 3306
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2213 3554 1452
Size: 761 1341 1452
Fragments arranged by size:
1452 1341 761
BclI T'GATC_A
Cuts at: 1074 1074
Size: 3554
BglII A'GATC_T
Cuts at: 1069 3106 1069
Size: 2037 1517
BlpI GC'TnA_GC
Cuts at: 666 666
Size: 3554
Bme1580I G_kGCm'C
Cuts at: 1179 1272 2328 1179
Size: 93 1056 2405
Fragments arranged by size:
2405 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 766 847 1182 3326 766
Size: 81 335 2144 994
Fragments arranged by size:
2144 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1609 2101 2399 730
Size: 879 492 298 1885
Fragments arranged by size:
1885 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 3258 295
Size: 2963 591
BsaAI yAC'GTr
Cuts at: 907 907
Size: 3554
BsaBI GATnn'nnATC
Cuts at: 6 1068 3105 6
Size: 1062 2037 455
Fragments arranged by size:
2037 1062 455
BsaHI Gr'CG_yC
Cuts at: 401 985 1233 1935 3431 401
Size: 584 248 702 1496 524
Fragments arranged by size:
1496 702 584 524 248
BsaWI w'CCGG_w
Cuts at: 465 743 1264 1942 2216 2363 2774 465
Size: 278 521 678 274 147 411 1245
Fragments arranged by size:
1245 678 521 411 278 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 117 147 301 331 117
Size: 30 154 30 3340
Fragments arranged by size:
3340 154 30 30
BseYI C'CCAG_C
Cuts at: 348 979 1737 2314 2810 348
Size: 631 758 577 496 1092
Fragments arranged by size:
1092 758 631 577 496
BsiEI CG_ry'CG
Cuts at: 104 1096 1142 2350 104
Size: 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 46
BsiHKAI G_wGCw'C
Cuts at: 568 1346 1536 2328 568
Size: 778 190 792 1794
Fragments arranged by size:
1794 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 539 568 1179 1272 1346 1536 1598 2328 539
Size: 29 611 93 74 190 62 730 1765
Fragments arranged by size:
1765 730 611 190 93 74 62 29
BspEI T'CCGG_A
Cuts at: 2774 2774
Size: 3554
BspHI T'CATG_A
Cuts at: 200 2730 200
Size: 2530 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 604 818 1120 1501 284
Size: 320 214 302 381 2337
Fragments arranged by size:
2337 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 1846 1900 3384 3549 50
Size: 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 1466 522
Size: 944 2610
BsrFI r'CCGG_y
Cuts at: 1552 1733 1942 1552
Size: 181 209 3164
Fragments arranged by size:
3164 209 181
BssSI C'ACGA_G
Cuts at: 1825 2183 1825
Size: 358 3196
BstAPI GCAn_nnn'nTGC
Cuts at: 118 362 931 118
Size: 244 569 2741
Fragments arranged by size:
2741 569 244
BstBI TT'CG_AA
Cuts at: 1915 1915
Size: 3554
BstYI r'GATC_y
Cuts at: 1 328 579 1046 1069 1404 1650 2662 2748
Size: 327 251 467 23 335 246 1012 86
Cuts at: 2748 2760 3106 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 327 251 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1504 1685 1848 1504
Size: 181 163 3210
Fragments arranged by size:
3210 181 163
BtsI GCAGTG_nn'
Cuts at: 797 797
Size: 3554
DraI TTT'AAA
Cuts at: 553 553
Size: 3554
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2118 1260
Size: 858 2696
EaeI y'GGCC_r
Cuts at: 1139 1313 1704 1731 3076 1139
Size: 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1139 1139
Size: 3554
EarI CTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1762 2072 2218 3364 1762
Size: 310 146 1146 1952
Fragments arranged by size:
1952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 566
Size: 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1811 2558 2826 1379
Size: 432 747 268 2107
Fragments arranged by size:
2107 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 115 139 693 115
Size: 24 554 2976
Fragments arranged by size:
2976 554 24
EcoRI G'AATT_C
Cuts at: 558 558
Size: 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 1573
Size: 3554
FspI TGC'GCA
Cuts at: 1335 1335
Size: 3554
HaeII r_GCGC'y
Cuts at: 988 1236 2258 3237 988
Size: 248 1022 979 1305
Fragments arranged by size:
1305 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 117 123 149 155 320 352 474 506 1396
Size: 6 26 6 165 32 122 32 890
Cuts at: 1396 1426 1428 1458 117
Size: 30 2 30 2213
Fragments arranged by size:
2213 890 165 122 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 593 593
Size: 3554
KasI G'GCGC_C
Cuts at: 984 1232 984
Size: 248 3306
KpnI G_GTAC'C
Cuts at: 574 574
Size: 3554
MfeI C'AATT_G
Cuts at: 237 237
Size: 3554
MluI A'CGCG_T
Cuts at: 2987 2987
Size: 3554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2409 2225
Size: 184 3370
MscI TGG'CCA
Cuts at: 1315 1315
Size: 3554
MslI CAynn'nnrTG
Cuts at: 1543 1670 1543
Size: 127 3427
MspA1I CmG'CkG
Cuts at: 352 362 392 665 979 1339 2352 2597 352
Size: 10 30 273 314 360 1013 245 1309
Fragments arranged by size:
1309 1013 360 314 273 245 30 10
NaeI GCC'GGC
Cuts at: 1735 1735
Size: 3554
NarI GG'CG_CC
Cuts at: 985 1233 985
Size: 248 3306
NcoI C'CATG_G
Cuts at: 107 107
Size: 3554
NgoMIV G'CCGG_C
Cuts at: 1733 1733
Size: 3554
NspI r_CATG'y
Cuts at: 607 1107 2014 607
Size: 500 907 2147
Fragments arranged by size:
2147 907 500
PciI A'CATG_T
Cuts at: 2010 2010
Size: 3554
PflMI CCAn_nnn'nTGG
Cuts at: 135 135
Size: 3554
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 3554
PmeI GTTT'AAAC
Cuts at: 553 553
Size: 3554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 429 461 2693 2725 429
Size: 32 2232 32 1258
Fragments arranged by size:
2232 1258 32 32
PpuMI rG'GwC_Cy
Cuts at: 139 139
Size: 3554
PstI C_TGCA'G
Cuts at: 123 601 1286 123
Size: 478 685 2391
Fragments arranged by size:
2391 685 478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3554
PvuII CAG'CTG
Cuts at: 352 362 979 1339 352
Size: 10 617 360 2567
Fragments arranged by size:
2567 617 360 10
RsrII CG'GwC_CG
Cuts at: 1749 1749
Size: 3554
SacI G_AGCT'C
Cuts at: 568 568
Size: 3554
SalI G'TCGA_C
Cuts at: 591 591
Size: 3554
SanDI GG'GwC_CC
Cuts at: 139 139
Size: 3554
SapI GCTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
SbfI CC_TGCA'GG
Cuts at: 123 601 123
Size: 478 3076
ScaI AGT'ACT
Cuts at: 3071 3071
Size: 3554
SfcI C'TryA_G
Cuts at: 33 119 597 1282 2275 2466 33
Size: 86 478 685 993 191 1121
Fragments arranged by size:
1121 993 685 478 191 86
SfoI GGC'GCC
Cuts at: 986 1234 986
Size: 248 3306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
SmaI CCC'GGG
Cuts at: 576 576
Size: 3554
SmlI C'TyrA_G
Cuts at: 709 1624 2116 2378 709
Size: 915 492 262 1885
Fragments arranged by size:
1885 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3554
SphI G_CATG'C
Cuts at: 607 1107 607
Size: 500 3054
StyI C'CwwG_G
Cuts at: 107 305 688 1042 107
Size: 198 383 354 2619
Fragments arranged by size:
2619 383 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1270 1729 1938 1137
Size: 133 459 209 2753
Fragments arranged by size:
2753 459 209 133
TatI w'GTAC_w
Cuts at: 3069 3069
Size: 3554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 248 280 301 333 3071 3103 248
Size: 32 21 32 2738 32 699
Fragments arranged by size:
2738 699 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1351 1351
Size: 3554
XbaI T'CTAG_A
Cuts at: 585 585
Size: 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1049 1049
Size: 3554
XmaI C'CCGG_G
Cuts at: 574 574
Size: 3554
ZraI GAC'GTC
Cuts at: 402 402
Size: 3554
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI