Restriction map for pF1KB7240
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AciI          739  GCGG                 CCGC(-3/-1)
AcsI          508  AAATTT               [GA]^AATT[CT]
AlwI          757  GGATC                GGATC(4/5)
AlwNI          23  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          873  CCTGAGG              CC^T[ACGT]AGG
ApoI          508  AAATTT               [GA]^AATT[CT]
AspHI          64  GAGCTC               G[AT]GC[AT]^C
BalI          371  TGGCCA               TGG^CCA
BbvCI         186  CCTCAGC              CCTCAGC(-5/-2)
BciVI         817  GTATCC               GTATCC(6/5)
BfaI          569  CTAG                 C^TAG
BpmI           67  CTCCAG               CTGGAG(16/14)
BsgI          163  CTGCAC               GTGCAG(16/14)
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
BsmFI         625  GTCCC                GGGAC(10/14)
Bsp1407I      392  TGTACA               T^GTACA
BsrDI         349  GCAATG               GCAATG(2/0)
BsrGI         392  TGTACA               T^GTACA
BstYI         757  GGATCT               [GA]^GATC[CT]
Bsu36I        873  CCTGAGG              CC^T[ACGT]AGG
DsaI          800  CCATGG               C^C[GA][CT]GG
EaeI          371  TGGCCA               [CT]^GGCC[GA]
EarI           79  CTCTTC               CTCTTC(1/4)
Eco81I        873  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       800  CCATGG               C^C[AT][AT]GG
EcoT22I       288  ATGCAT               ATGCA^T
HincII        580  GTCAAC               GT[CT]^[GA]AC
HindII        580  GTCAAC               GT[CT]^[GA]AC
Ksp632I        79  CTCTTC               CTCTTC(1/4)
MaeI          569  CTAG                 C^TAG
MflI          757  GGATCT               [GA]^GATC[CT]
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
MspA1I         70  CAGCTG               C[AC]G^C[GT]G
NcoI          800  CCATGG               C^CATGG
NsiI          288  ATGCAT               ATGCA^T
PflMI         729  CCACATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          294  GACTC                GAGTC(4/5)
PstI          761  CTGCAG               CTGCA^G
PvuII          70  CAGCTG               CAG^CTG
SacI           64  GAGCTC               GAGCT^C
SspBI         392  TGTACA               T^GTACA
StuI          453  AGGCCT               AGG^CCT
StyI          800  CCATGG               C^C[AT][AT]GG
TfiI           98  GAATC                G^A[AT]TC
Van91I        729  CCACATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         757  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII        338  ACATGT               A^C[GA][CT]GT
              731  ACATGT              
AvaII         701  GGTCC                G^G[AT]CC
              804  GGACC               
BanII          46  GGGCTC               G[GA]GC[CT]^C
               64  GAGCTC              
BsiYI         363  CCGTTACATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              729  CCACATGTTGG         
BslI          363  CCGTTACATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              729  CCACATGTTGG         
BsmI          286  GAATGC               GAATGC(1/-1)
              766  GCATTC              
BspLU11I      338  ACATGT               A^CATGT
              731  ACATGT              
BspMI         161  ACCTGC               ACCTGC(4/8)
              698  GCAGGT              
Cac8I         418  GCTTGC               GC[ACGT]^[ACGT]GC
              667  GCCAGC              
Cfr13I        701  GGTCC                G^G[ACGT]CC
              804  GGACC               
DpnII         538  GATC                 ^GATC
              758  GATC                
HaeIII        372  GGCC                 GG^CC
              454  GGCC                
HinfI          98  GAATC                G^A[ACGT]TC
              294  GACTC               
HphI          246  GGTGA                GGTGA(8/7)
              159  TCACC               
MboI          538  GATC                 ^GATC
              758  GATC                
MseI          521  TTAA                 T^TAA
              614  TTAA                
MslI          111  CACAGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              168  CACCCCTATG          
NdeII         538  GATC                 ^GATC
              758  GATC                
NspI          338  ACATGT               [GA]CATG^[CT]
              731  ACATGT              
Sau3AI        538  GATC                 ^GATC
              758  GATC                
Sau96I        701  GGTCC                G^G[ACGT]CC
              804  GGACC               
Tru9I         521  TTAA                 T^TAA
              614  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
Bsp1286I    BssKI       BstF5I      BstNI       DdeI        
EcoRII      Fnu4HI      FokI        ItaI        MaeIII      
MboII       MnlI        MvaI        MwoI        NlaIII      
RsaI        ScrFI       SfaNI       SfcI        TseI        
Tsp45I      Tsp509I     TspRI       
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