Restriction map for pF1KB7240
 (Linear) (Six-base) MAPSORT of: KIBB7240.seq  Check: 483  from: 1  to: 933



KIBB7240.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     170     933 
   Size:        170     763
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     696     922     933 
   Size:        696     226      11

  Fragments arranged by size: 

                 696     226      11
 
AflIII A'CryG_T

Cuts at:      0     338     731     933 
   Size:        338     393     202

  Fragments arranged by size: 

                 393     338     202
 
AleI CACnn'nnGTG

Cuts at:      0     115     933 
   Size:        115     818
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     259     291     933 
   Size:        259      32     642

  Fragments arranged by size: 

                 642     259      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      28     933 
   Size:         28     905
 
ApoI r'AATT_y

Cuts at:      0     508     933 
   Size:        508     425
 
BanII G_rGCy'C

Cuts at:      0      50      68     933 
   Size:         50      18     865

  Fragments arranged by size: 

                 865      50      18
 
BbvCI CC'TCA_GC

Cuts at:      0     187     933 
   Size:        187     746
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     828     933 
   Size:        828     105
 
BfrBI ATG'CAT

Cuts at:      0     290     933 
   Size:        290     643
 
Bme1580I G_kGCm'C

Cuts at:      0     848     933 
   Size:        848      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     933 
   Size:         52     881
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     933 
   Size:        187     746
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     670     700     933 
   Size:        259      30     381      30     233

  Fragments arranged by size: 

                 381     259     233      30      30
 
BseYI C'CCAG_C

Cuts at:      0      43     628     933 
   Size:         43     585     305

  Fragments arranged by size: 

                 585     305      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     933 
   Size:        148     785
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     933 
   Size:         68     865
 
BsmI GAATG_Cn'

Cuts at:      0     292     766     933 
   Size:        292     474     167

  Fragments arranged by size: 

                 474     292     167
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      68     848     933 
   Size:         50      18     780      85

  Fragments arranged by size: 

                 780      85      50      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     689     933 
   Size:        170     519     244

  Fragments arranged by size: 

                 519     244     170
 
BsrDI GCAATG_nn'

Cuts at:      0     356     933 
   Size:        356     577
 
BsrGI T'GTAC_A

Cuts at:      0     392     933 
   Size:        392     541
 
BstYI r'GATC_y

Cuts at:      0     757     933 
   Size:        757     176
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     933 
   Size:        874      59
 
BtgI C'CryG_G

Cuts at:      0     800     933 
   Size:        800     133
 
EaeI y'GGCC_r

Cuts at:      0     371     933 
   Size:        371     562
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     933 
   Size:         85     848
 
EcoICRI GAG'CTC

Cuts at:      0      66     933 
   Size:         66     867
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     696     922     933 
   Size:         52     644     226      11

  Fragments arranged by size: 

                 644     226      52      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     438     933 
   Size:        438     495
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     685     717     933 
   Size:        685      32     216

  Fragments arranged by size: 

                 685     216      32
 
HincII GTy'rAC

Cuts at:      0     582     933 
   Size:        582     351
 
MscI TGG'CCA

Cuts at:      0     373     933 
   Size:        373     560
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     933 
   Size:        115      57     761

  Fragments arranged by size: 

                 761     115      57
 
MspA1I CmG'CkG

Cuts at:      0      72     933 
   Size:         72     861
 
NcoI C'CATG_G

Cuts at:      0     800     933 
   Size:        800     133
 
NsiI A_TGCA'T

Cuts at:      0     292     933 
   Size:        292     641
 
NspI r_CATG'y

Cuts at:      0     342     735     933 
   Size:        342     393     198

  Fragments arranged by size: 

                 393     342     198
 
PciI A'CATG_T

Cuts at:      0     338     731     933 
   Size:        338     393     202

  Fragments arranged by size: 

                 393     338     202
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     735     933 
   Size:        735     198
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     933 
   Size:        259      32     642

  Fragments arranged by size: 

                 642     259      32
 
PstI C_TGCA'G

Cuts at:      0     765     933 
   Size:        765     168
 
PvuII CAG'CTG

Cuts at:      0      72     933 
   Size:         72     861
 
SacI G_AGCT'C

Cuts at:      0      68     933 
   Size:         68     865
 
SfcI C'TryA_G

Cuts at:      0     378     761     913     933 
   Size:        378     383     152      20

  Fragments arranged by size: 

                 383     378     152      20
 
StuI AGG'CCT

Cuts at:      0     455     933 
   Size:        455     478
 
StyI C'CwwG_G

Cuts at:      0     800     933 
   Size:        800     133
 
TatI w'GTAC_w

Cuts at:      0     176     392     773     933 
   Size:        176     216     381     160

  Fragments arranged by size: 

                 381     216     176     160
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      89     121     670     702     933 
   Size:         89      32     549      32     231

  Fragments arranged by size: 

                 549     231      89      32      32
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI     ApoI    BanII
    BbvCI    BciVI    BfrBI Bme1580I     BpmI   Bpu10I    BsaXI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrGI    BstYI
   Bsu36I     BtgI     EaeI     EarI  EcoICRI  Eco57MI     FalI    Hin4I
   HincII     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PciI
    PflMI     PpiI     PstI    PvuII     SacI     SfcI     StuI     StyI
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI     BcgI     BclI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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