Restriction map for
pF1KB7239
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I DdeI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MscI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 98 GGTACC G^GTACC
AccIII 635 TCCGGA T^CCGGA
AluI 644 AGCT AG^CT
AlwNI 559 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 98 GGTACC G^GTACC
BamHI 404 GGATCC G^GATCC
BbsI 523 GTCTTC GAAGAC(2/6)
BglII 375 AGATCT A^GATCT
BpmI 649 CTGGAG CTGGAG(16/14)
BpuAI 523 GTCTTC GAAGAC(2/6)
BseAI 635 TCCGGA T^CCGGA
BsgI 457 GTGCAG GTGCAG(16/14)
BsiYI 408 CCTCACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 408 CCTCACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 184 GAATGC GAATGC(1/-1)
BspDI 430 ATCGAT AT^CGAT
BspEI 635 TCCGGA T^CCGGA
BsrDI 627 CATTGC GCAATG(2/0)
Bst1107I 176 GTATAC GTA^TAC
BstZ17I 176 GTATAC GTA^TAC
ClaI 430 ATCGAT AT^CGAT
CpoI 539 CGGACCG CG^G[AT]CCG
DsaI 551 CCACGG C^C[GA][CT]GG
EcoNI 408 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 656 GCGTC GACGC(5/10)
HinfI 214 GAGTC G^A[ACGT]TC
KpnI 98 GGTACC GGTAC^C
MaeII 620 ACGT A^CGT
MroI 635 TCCGGA T^CCGGA
MspA1I 607 CCGCTG C[AC]G^C[GT]G
NlaIII 110 CATG CATG^
NspI 109 GCATGC [GA]CATG^[CT]
PleI 214 GAGTC GAGTC(4/5)
PstI 152 CTGCAG CTGCA^G
RsrII 539 CGGACCG CG^G[AT]CCG
SfcI 152 CTGCAG C^T[GA][CT]AG
SphI 109 GCATGC GCATG^C
TspRI 361 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 69 GTCTAC GT^[AC][GT]AC
176 GTATAC
AlwI 405 GATCC GGATC(4/5)
404 GGATC
BanI 98 GGTACC G^G[CT][GA]CC
157 GGTGCC
BbvI 567 GCTGC GCAGC(8/12)
645 GCTGC
BsaWI 231 ACCGGA [AT]^CCGG[AT]
635 TCCGGA
BstXI 161 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCATCCGGATGG
BstYI 375 AGATCT [GA]^GATC[CT]
404 GGATCC
CfoI 420 GCGC GCG^C
477 GCGC
DraII 89 AGGACCC [GA]G^G[ACGT]CC[CT]
132 AGGACCC
EcoO109I 89 AGGACCC [GA]G^G[ACGT]CC[CT]
132 AGGACCC
EcoT14I 126 CCAAGG C^C[AT][AT]GG
167 CCTTGG
FauI 587 CCCGC CCCGC(4/6)
606 CCCGC
HaeII 419 GGCGCT [GA]GCGC^[CT]
476 AGCGCC
HaeIII 300 GGCC GG^CC
572 GGCC
HhaI 420 GCGC GCG^C
477 GCGC
HinP1I 420 GCGC G^CGC
477 GCGC
HphI 242 TCACC GGTGA(8/7)
410 TCACC
MboII 280 TCTTC GAAGA(8/7)
524 TCTTC
MflI 375 AGATCT [GA]^GATC[CT]
404 GGATCC
MslI 110 CATGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
633 CATCCGGATG
PpuMI 89 AGGACCC [GA]G^G[AT]CC[CT]
132 AGGACCC
StyI 126 CCAAGG C^C[AT][AT]GG
167 CCTTGG
TaqI 212 TCGA T^CGA
431 TCGA
TseI 567 GCTGC G^C[AT]GC
645 GCTGC
TthHB8I 212 TCGA T^CGA
431 TCGA
XhoII 375 AGATCT [GA]^GATC[CT]
404 GGATCC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AvaII BcnI BsaJI
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HapII HpaII ItaI MaeIII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI Tsp45I