Restriction map for pF1KB7236
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstXI       BstYI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SfaNI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII         917  CGCG                 CG^CG
AflIII        197  ACGTGT               A^C[GA][CT]GT
Aor51HI        92  AGCGCT               AGC^GCT
ApaI          289  GGGCCC               GGGCC^C
BalI          107  TGGCCA               TGG^CCA
BcgI          456  CGATGTCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         236  GTATCC               GTATCC(6/5)
BclI          416  TGATCA               T^GATCA
BlpI          303  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      303  GCTCAGC              GC^T[ACGT]AGC
BsaI          631  GAGACC               GGTCTC(1/5)
BsmBI         358  CGTCTC               CGTCTC(1/5)
BspMI         594  GCAGGT               ACCTGC(4/8)
BstBI         496  TTCGAA               TT^CGAA
BstEII        564  GGTCACC              G^GT[ACGT]ACC
BstF5I        774  CATCC                GGATG(2/0)
BstPI         564  GGTCACC              G^GT[ACGT]ACC
BstUI         917  CGCG                 CG^CG
Cac8I         434  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         303  GCTCAGC              GC^T[ACGT]AGC
DraIII        406  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III       92  AGCGCT               AGC^GCT
EcoO65I       564  GGTCACC              G^GT[ACGT]ACC
EcoT14I       879  CCTTGG               C^C[AT][AT]GG
FauI          918  GCGGG                CCCGC(4/6)
FbaI          416  TGATCA               T^GATCA
FokI          774  CATCC                GGATG(9/13)
HaeII          92  AGCGCT               [GA]GCGC^[CT]
HincII        727  GTCAAC               GT[CT]^[GA]AC
HindII        727  GTCAAC               GT[CT]^[GA]AC
HindIII       583  AAGCTT               A^AGCTT
MaeIII        565  GTCAC                ^GT[ACGT]AC
MluNI         107  TGGCCA               TGG^CCA
MscI          107  TGGCCA               TGG^CCA
MslI          408  CATCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          917  CGCG                 CG^CG
NspV          496  TTCGAA               TT^CGAA
SbfI          435  CCTGCAGG             CCTGCA^GG
SexAI          98  ACCAGGT              A^CC[AT]GGT
SfuI          496  TTCGAA               TT^CGAA
Sse8387I      435  CCTGCAGG             CCTGCA^GG
StyI          879  CCTTGG               C^C[AT][AT]GG
Tsp45I        565  GTCAC                ^GT[GC]AC
Tsp509I        48  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII         120  GGACC                G^G[AT]CC
              651  GGACC               
BanII         289  GGGCCC               G[GA]GC[CT]^C
              301  GGGCTC              
BcnI           14  CCCGG                CC^[GC]GG
              648  CCGGG               
BmyI          289  GGGCCC               G[AGT]GC[ACT]^C
              301  GGGCTC              
Bsp1286I      289  GGGCCC               G[AGT]GC[ACT]^C
              301  GGGCTC              
DpnII          27  GATC                 ^GATC
              417  GATC                
EarI          853  CTCTTC               CTCTTC(1/4)
              387  GAAGAG              
Ksp632I       853  CTCTTC               CTCTTC(1/4)
              387  GAAGAG              
MboI           27  GATC                 ^GATC
              417  GATC                
NciI           14  CCCGG                CC^[GC]GG
              648  CCGGG               
NdeII          27  GATC                 ^GATC
              417  GATC                
PflMI          99  CCAGGTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              405  CCACATCGTGG         
PleI          744  GACTC                GAGTC(4/5)
              211  GAGTC               
PpuMI         119  GGGACCT              [GA]G^G[AT]CC[CT]
              650  GGGACCT             
PstI          436  CTGCAG               CTGCA^G
              520  CTGCAG              
Sau3AI         27  GATC                 ^GATC
              417  GATC                
SfcI          436  CTGCAG               C^T[GA][CT]AG
              520  CTGCAG              
TfiI          491  GATTC                G^A[AT]TC
              811  GATTC               
Van91I         99  CCAGGTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              405  CCACATCGTGG         
XcmI          113  CCATGAGGGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              473  CCAAGTTCGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwNI       BbvI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsrI        BssKI       BstNI       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NlaIII      
NlaIV       PvuII       Sau96I      ScrFI       TaqI        
TseI        TspRI       TthHB8I     
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