Restriction map for pF1KB7236
 (Linear) (Six-base) MAPSORT of: KIBB7236.seq  Check: 7051  from: 1  to: 933



KIBB7236.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AfeI AGC'GCT

Cuts at:      0      94     933 
   Size:         94     839
 
AflIII A'CryG_T

Cuts at:      0     197     933 
   Size:        197     736
 
AlwNI CAG_nnn'CTG

Cuts at:      0     154     519     622     933 
   Size:        154     365     103     311

  Fragments arranged by size: 

                 365     311     154     103
 
ApaI G_GGCC'C

Cuts at:      0     293     933 
   Size:        293     640
 
BanII G_rGCy'C

Cuts at:      0     293     305     933 
   Size:        293      12     628

  Fragments arranged by size: 

                 628     293      12
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445     479     933 
   Size:        445      34     454

  Fragments arranged by size: 

                 454     445      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     247     933 
   Size:        247     686
 
BclI T'GATC_A

Cuts at:      0     416     933 
   Size:        416     517
 
BlpI GC'TnA_GC

Cuts at:      0     304     933 
   Size:        304     629
 
Bme1580I G_kGCm'C

Cuts at:      0     293     933 
   Size:        293     640
 
BmgBI CAC'GTC

Cuts at:      0     198     255     711     933 
   Size:        198      57     456     222

  Fragments arranged by size: 

                 456     222     198      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     216     933 
   Size:        216     717
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     316     505     654     933 
   Size:        163     153     189     149     279

  Fragments arranged by size: 

                 279     189     163     153     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     625     933 
   Size:        625     308
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     405     841     862     933 
   Size:        405     436      21      71

  Fragments arranged by size: 

                 436     405      71      21
 
BseYI C'CCAG_C

Cuts at:      0     154     188     663     933 
   Size:        154      34     475     270

  Fragments arranged by size: 

                 475     270     154      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     622     933 
   Size:        222     400     311

  Fragments arranged by size: 

                 400     311     222
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     364     933 
   Size:        364     569
 
Bsp1286I G_dGCh'C

Cuts at:      0     293     305     933 
   Size:        293      12     628

  Fragments arranged by size: 

                 628     293      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     585     933 
   Size:        585     348
 
BstBI TT'CG_AA

Cuts at:      0     497     933 
   Size:        497     436
 
BstEII G'GTnAC_C

Cuts at:      0     564     933 
   Size:        564     369
 
BtgI C'CryG_G

Cuts at:      0     182     560     689     806     933 
   Size:        182     378     129     117     127

  Fragments arranged by size: 

                 378     182     129     127     117
 
BtsI GCAGTG_nn'

Cuts at:      0      71     210     429     933 
   Size:         71     139     219     504

  Fragments arranged by size: 

                 504     219     139      71
 
DraIII CAC_nnn'GTG

Cuts at:      0     411     933 
   Size:        411     522
 
EaeI y'GGCC_r

Cuts at:      0       2     107     925     933 
   Size:          2     105     818       8

  Fragments arranged by size: 

                 818     105       8       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     859     933 
   Size:        382     477      74

  Fragments arranged by size: 

                 477     382      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     316     505     654     933 
   Size:        163     153     189     149     279

  Fragments arranged by size: 

                 279     189     163     153     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     289     290     556     651     933 
   Size:        120     169       1     266      95     282

  Fragments arranged by size: 

                 282     266     169     120      95       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     391     933 
   Size:        391     542
 
HaeII r_GCGC'y

Cuts at:      0      96     933 
   Size:         96     837
 
HincII GTy'rAC

Cuts at:      0     729     933 
   Size:        729     204
 
HindIII A'AGCT_T

Cuts at:      0     583     933 
   Size:        583     350
 
MscI TGG'CCA

Cuts at:      0     109     933 
   Size:        109     824
 
MslI CAynn'nnrTG

Cuts at:      0     412     933 
   Size:        412     521
 
MspA1I CmG'CkG

Cuts at:      0       7     151     192     233     516     789     860     933 
   Size:          7     144      41      41     283     273      71      73

  Fragments arranged by size: 

                 283     273     144      73      71      41      41       7
            
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     105     411     933 
   Size:        105     306     522

  Fragments arranged by size: 

                 522     306     105
 
PfoI T'CCnGG_A

Cuts at:      0     647     670     933 
   Size:        647      23     263

  Fragments arranged by size: 

                 647     263      23
 
PpuMI rG'GwC_Cy

Cuts at:      0     120     651     933 
   Size:        120     531     282

  Fragments arranged by size: 

                 531     282     120
 
PspOMI G'GGCC_C

Cuts at:      0     289     933 
   Size:        289     644
 
PstI C_TGCA'G

Cuts at:      0     440     524     933 
   Size:        440      84     409

  Fragments arranged by size: 

                 440     409      84
 
PvuII CAG'CTG

Cuts at:      0     151     192     233     516     933 
   Size:        151      41      41     283     417

  Fragments arranged by size: 

                 417     283     151      41      41
 
SbfI CC_TGCA'GG

Cuts at:      0     440     933 
   Size:        440     493
 
SexAI A'CCwGG_T

Cuts at:      0      98     933 
   Size:         98     835
 
SfcI C'TryA_G

Cuts at:      0     436     520     933 
   Size:        436      84     413

  Fragments arranged by size: 

                 436     413      84
 
StyI C'CwwG_G

Cuts at:      0     879     933 
   Size:        879      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     480     933 
   Size:        120     360     453

  Fragments arranged by size: 

                 453     360     120
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI     ApaI    BanII     BcgI    BciVI     BclI
     BlpI Bme1580I    BmgBI     BmrI     BpmI     BsaI    BseRI    BseYI
     BsgI    BsmBI Bsp1286I    BspMI    BstBI   BstEII     BtgI     BtsI
   DraIII     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII   HincII
  HindIII     MscI     MslI   MspA1I    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SbfI    SexAI     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI    BfrBI     BglI    BglII     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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