Restriction map for pF1KB7232
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspHI       AvrII       
BamHI       BbeI        BbrPI       BciVI       BglI        
BglII       BlnI        BpmI        BsaBI       BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        HaeII       HindIII     
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PleI        PmaCI       PmeI        PmlI        Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

AccIII         75  TCCGGA               T^CCGGA
AcsI          553  AAATTC               [GA]^AATT[CT]
AflII         874  CTTAAG               C^TTAAG
AflIII        726  ACATGT               A^C[GA][CT]GT
AgeI          290  ACCGGT               A^CCGGT
AhdI           12  GACCCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1069  CCTAAGG              CC^T[ACGT]AGG
ApoI          553  AAATTC               [GA]^AATT[CT]
AseI          511  ATTAAT               AT^TAAT
AsnI          511  ATTAAT               AT^TAAT
AspEI          12  GACCCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          139  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          951  CCCGGG               C^[CT]CG[GA]G
AviII         326  TGCGCA               TGC^GCA
BalI          801  TGGCCA               TGG^CCA
BanI          222  GGTGCC               G^G[CT][GA]CC
BanII         270  GGGCTC               G[GA]GC[CT]^C
BbsI           20  GTCTTC               GAAGAC(2/6)
BbvCI         437  CCTCAGC              CCTCAGC(-5/-2)
BcgI           89  GCAGGAAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1002  TGATCA               T^GATCA
BfrI          874  CTTAAG               C^TTAAG
BlpI          171  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      171  GCTCAGC              GC^T[ACGT]AGC
BpuAI          20  GTCTTC               GAAGAC(2/6)
BsaAI         904  TACGTG               [CT]AC^GT[GA]
BsaI          195  GAGACC               GGTCTC(1/5)
BseAI          75  TCCGGA               T^CCGGA
BsgI          650  CTGCAC               GTGCAG(16/14)
BsoBI         951  CCCGGG               C^[CT]CG[GA]G
BspEI          75  TCCGGA               T^CCGGA
BspLU11I      726  ACATGT               A^CATGT
BsrDI         785  CATTGC               GCAATG(2/0)
BsrFI         290  ACCGGT               [GA]^CCGG[CT]
BsrI          935  ACTGG                ACTGG(1/-1)
BstXI         566  CCAGACTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1069  CCTAAGG              CC^T[ACGT]AGG
CelII         171  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        290  ACCGGT               [GA]^CCGG[CT]
DpnII        1003  GATC                 ^GATC
Eam1105I       12  GACCCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1069  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       669  CCATGG               C^C[AT][AT]GG
FbaI         1002  TGATCA               T^GATCA
FspI          326  TGCGCA               TGC^GCA
HpaI         1054  GTTAAC               GTT^AAC
MboI         1003  GATC                 ^GATC
MluNI         801  TGGCCA               TGG^CCA
MroI           75  TCCGGA               T^CCGGA
MscI          801  TGGCCA               TGG^CCA
NcoI          669  CCATGG               C^CATGG
NdeII        1003  GATC                 ^GATC
PflFI         139  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         288  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         290  ACCGGT               A^CCGGT
PshAI        1038  GACGTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         511  ATTAAT               AT^TAAT
Sau3AI       1003  GATC                 ^GATC
SexAI         580  ACCAGGT              A^CC[AT]GGT
SmaI          951  CCCGGG               CCC^GGG
SmlI          874  CTTAAG               C^T[CT][GA]AG
SphI         1075  GCATGC               GCATG^C
SspI          514  AATATT               AAT^ATT
StyI          669  CCATGG               C^C[AT][AT]GG
Tth111I       139  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        288  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         951  CCCGGG               C^CCGGG
XmaI          951  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          60  CGCG                 CG^CG
               99  CGCG                
AcyI          105  GGCGTC               G[GA]^CG[CT]C
              214  GACGCC              
AfaI          903  GTAC                 GT^AC
             1116  GTAC                
BmyI          223  GTGCCC               G[AGT]GC[ACT]^C
              270  GGGCTC              
BsaHI         105  GGCGTC               G[GA]^CG[CT]C
              214  GACGCC              
BsaWI          75  TCCGGA               [AT]^CCGG[AT]
              290  ACCGGT              
BseRI         424  GAGGAG               GAGGAG(10/8)
              700  GAGGAG              
BsmI          597  GAATGC               GAATGC(1/-1)
              982  GAATGC              
Bsp1286I      223  GTGCCC               G[AGT]GC[ACT]^C
              270  GGGCTC              
BstF5I        125  CATCC                GGATG(2/0)
              820  CATCC               
BstUI          60  CGCG                 CG^CG
               99  CGCG                
CfoI           59  GCGC                 GCG^C
              327  GCGC                
EaeI            5  TGGCCG               [CT]^GGCC[GA]
              801  TGGCCA              
FokI          125  CATCC                GGATG(9/13)
              820  CATCC               
HgaI          214  GACGC                GACGC(5/10)
              106  GCGTC               
HhaI           59  GCGC                 GCG^C
              327  GCGC                
Hin1I         105  GGCGTC               G[GA]^CG[CT]C
              214  GACGCC              
HinP1I         59  GCGC                 G^CGC
              327  GCGC                
HincII       1045  GTCAAC               GT[CT]^[GA]AC
             1054  GTTAAC              
HindII       1045  GTCAAC               GT[CT]^[GA]AC
             1054  GTTAAC              
MslI          751  CACCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1161  CATGGTTGTG          
MvnI           60  CGCG                 CG^CG
               99  CGCG                
PvuII         765  CAGCTG               CAG^CTG
              882  CAGCTG              
RsaI          903  GTAC                 GT^AC
             1116  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BspMI       BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DraII       DsaI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       NspI        PpuMI       Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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