Restriction map for pF1KB7219
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BmyI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI           24  GTCGAC               GT^[AC][GT]AC
AciI          363  CCGC                 CCGC(-3/-1)
AlwI          111  GATCC                GGATC(4/5)
AspI          597  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          415  GGTGCC               G^G[CT][GA]CC
BbsI          817  GAAGAC               GAAGAC(2/6)
BlpI          722  GCTCAGC              GC^T[ACGT]AGC
BpmI           67  CTCCAG               CTGGAG(16/14)
Bpu1102I      722  GCTCAGC              GC^T[ACGT]AGC
BpuAI         817  GAAGAC               GAAGAC(2/6)
BsmFI         232  GTCCC                GGGAC(10/14)
BspHI         404  TCATGA               T^CATGA
BsrDI         487  GCAATG               GCAATG(2/0)
BstXI         248  CCAGCTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         247  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         722  GCTCAGC              GC^T[ACGT]AGC
DraIII        327  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
HincII         24  GTCGAC               GT[CT]^[GA]AC
HindII         24  GTCGAC               GT[CT]^[GA]AC
MslI          168  CACCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        434  CAGCTG               C[AC]G^C[GT]G
PflFI         597  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         439  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         434  CAGCTG               CAG^CTG
RcaI          404  TCATGA               T^CATGA
SalI           24  GTCGAC               G^TCGAC
SfcI          885  CTATAG               C^T[GA][CT]AG
StuI          707  AGGCCT               AGG^CCT
Tsp45I        448  GTCAC                ^GT[GC]AC
Tth111I       597  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcsI           31  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
AfaI          102  GTAC                 GT^AC
              774  GTAC                
ApoI           31  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
BalI          371  TGGCCA               TGG^CCA
              425  TGGCCA              
BbvI          433  GCAGC                GCAGC(8/12)
              913  GCTGC               
BglI          316  GCCATGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              418  GCCTATGTGGC         
BsgI          163  CTGCAC               GTGCAG(16/14)
              472  GTGCAG              
BspMI         161  ACCTGC               ACCTGC(4/8)
              718  ACCTGC              
BssKI          39  CCTGG                ^CC[ACGT]GG
              443  CCTGG               
BstF5I         98  GGATG                GGATG(2/0)
              903  GGATG               
BstNI          39  CCTGG                CC^[AT]GG
              443  CCTGG               
DpnII         111  GATC                 ^GATC
              339  GATC                
DraII         439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              830  AGGCCCT             
EaeI          371  TGGCCA               [CT]^GGCC[GA]
              425  TGGCCA              
EcoO109I      439  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              830  AGGCCCT             
EcoRII         39  CCTGG                ^CC[AT]GG
              443  CCTGG               
EcoT14I       317  CCATGG               C^C[AT][AT]GG
              428  CCATGG              
Fnu4HI        433  GCAGC                GC^[ACGT]GC
              913  GCTGC               
FokI           98  GGATG                GGATG(9/13)
              903  GGATG               
HphI          741  GGTGA                GGTGA(8/7)
              159  TCACC               
ItaI          433  GCAGC                GC^[ACGT]GC
              913  GCTGC               
MaeII          22  ACGT                 A^CGT
              209  ACGT                
MaeIII        448  GTCAC                ^GT[ACGT]AC
              495  GTTAC               
MboI          111  GATC                 ^GATC
              339  GATC                
MluNI         371  TGGCCA               TGG^CCA
              425  TGGCCA              
MscI          371  TGGCCA               TGG^CCA
              425  TGGCCA              
MseI          521  TTAA                 T^TAA
              872  TTAA                
MvaI           39  CCTGG                CC^[AT]GG
              443  CCTGG               
NcoI          317  CCATGG               C^CATGG
              428  CCATGG              
NdeII         111  GATC                 ^GATC
              339  GATC                
RsaI          102  GTAC                 GT^AC
              774  GTAC                
Sau3AI        111  GATC                 ^GATC
              339  GATC                
ScrFI          39  CCTGG                CC^[ACGT]GG
              443  CCTGG               
StyI          317  CCATGG               C^C[AT][AT]GG
              428  CCATGG              
Tru9I         521  TTAA                 T^TAA
              872  TTAA                
TseI          433  GCAGC                G^C[AT]GC
              913  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AvaII       BsaJI       BsiYI       BslI        
BsrI        Cfr13I      DdeI        DsaI        HaeIII      
HinfI       MboII       MnlI        MwoI        NlaIII      
NlaIV       PleI        Sau96I      TaqI        TfiI        
Tsp509I     TspRI       TthHB8I     
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