Restriction map for pF1KB7213
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        AlwI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstF5I      BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcyI          209  GGCGTC               G[GA]^CG[CT]C
AfaI          114  GTAC                 GT^AC
Alw44I        402  GTGCAC               G^TGCAC
AlwNI         304  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          409  GGGCCC               GGGCC^C
ApaLI         402  GTGCAC               G^TGCAC
AspHI         402  GTGCAC               G[AT]GC[AT]^C
BanI          361  GGCACC               G^G[CT][GA]CC
BbrPI          13  CACGTG               CAC^GTG
BpmI          214  CTGGAG               CTGGAG(16/14)
BsaAI          13  CACGTG               [CT]AC^GT[GA]
BsaHI         209  GGCGTC               G[GA]^CG[CT]C
BsaJI         357  CCTGGG               C^C[ACGT][ACGT]GG
BsiHKAI       402  GTGCAC               G[AT]GC[AT]^C
BstAPI        275  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         48  GGTCACC              G^GT[ACGT]ACC
BstPI          48  GGTCACC              G^GT[ACGT]ACC
CfoI          284  GCGC                 GCG^C
DpnII          81  GATC                 ^GATC
DrdI           93  GACAGGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          157  TGGCCG               [CT]^GGCC[GA]
EcoO65I        48  GGTCACC              G^GT[ACGT]ACC
FauI          417  CCCGC                CCCGC(4/6)
HgaI          210  GCGTC                GACGC(5/10)
HhaI          284  GCGC                 GCG^C
Hin1I         209  GGCGTC               G[GA]^CG[CT]C
HinP1I        284  GCGC                 G^CGC
MboI           81  GATC                 ^GATC
MboII          87  GAAGA                GAAGA(8/7)
MspA1I        304  CAGCGG               C[AC]G^C[GT]G
NaeI          286  GCCGGC               GCC^GGC
NdeII          81  GATC                 ^GATC
NgoMIV        286  GCCGGC               G^CCGGC
PflMI          12  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          13  CACGTG               CAC^GTG
PmlI           13  CACGTG               CAC^GTG
PstI          109  CTGCAG               CTGCA^G
RsaI          114  GTAC                 GT^AC
Sau3AI         81  GATC                 ^GATC
SexAI          59  ACCTGGT              A^CC[AT]GGT
SfaNI         140  GCATC                GCATC(5/9)
SfcI          109  CTGCAG               C^T[GA][CT]AG
TaqI           83  TCGA                 T^CGA
TspRI         154  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        83  TCGA                 T^CGA
Van91I         12  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI          221  AGCT                 AG^CT
              398  AGCT                
AvaII         165  GGACC                G^G[AT]CC
              293  GGACC               
BanII         232  GAGCCC               G[GA]GC[CT]^C
              409  GGGCCC              
BcnI           53  CCGGG                CC^[GC]GG
              407  CCGGG               
BspMI         298  ACCTGC               ACCTGC(4/8)
              313  ACCTGC              
BsrFI         159  GCCGGT               [GA]^CCGG[CT]
              286  GCCGGC              
Cac8I         286  GCCGGC               GC[ACGT]^[ACGT]GC
              302  GCCAGC              
Cfr10I        159  GCCGGT               [GA]^CCGG[CT]
              286  GCCGGC              
HaeIII        158  GGCC                 GG^CC
              410  GGCC                
HphI           75  GGTGA                GGTGA(8/7)
               50  TCACC               
MaeII          14  ACGT                 A^CGT
              270  ACGT                
NciI           53  CCGGG                CC^[GC]GG
              407  CCGGG               
Tsp45I         49  GTCAC                ^GT[GC]AC
              323  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        BbvI        BmyI        BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstNI       Cfr13I      
EcoRII      Fnu4HI      HapII       HpaII       ItaI        
MaeIII      MnlI        MspI        MvaI        MwoI        
NlaIV       Sau96I      ScrFI       TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com