Restriction map for pF1KB7209
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaHI       BsaI        
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FauI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MfeI        MflI        MluI        MluNI       
MscI        MseI        MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII         74  TCCGGA               T^CCGGA
AlwNI         412  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         339  GAGCTC               G[AT]GC[AT]^C
BbsI          100  GAAGAC               GAAGAC(2/6)
BclI          170  TGATCA               T^GATCA
BlpI          517  GCTGAGC              GC^T[ACGT]AGC
BpmI          334  CTCCAG               CTGGAG(16/14)
Bpu1102I      517  GCTGAGC              GC^T[ACGT]AGC
BpuAI         100  GAAGAC               GAAGAC(2/6)
BsaBI         171  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          74  TCCGGA               [AT]^CCGG[AT]
BseAI          74  TCCGGA               T^CCGGA
BsiHKAI       339  GAGCTC               G[AT]GC[AT]^C
BspEI          74  TCCGGA               T^CCGGA
CelII         517  GCTGAGC              GC^T[ACGT]AGC
CfoI          525  GCGC                 GCG^C
DpnII         171  GATC                 ^GATC
DrdI          475  GACCCCATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          479  CCATGG               C^C[GA][CT]GG
EcoNI         106  CCTTCTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       479  CCATGG               C^C[AT][AT]GG
EcoT22I       117  ATGCAT               ATGCA^T
FbaI          170  TGATCA               T^GATCA
HhaI          525  GCGC                 GCG^C
HinP1I        525  GCGC                 G^CGC
MaeII          79  ACGT                 A^CGT
MaeIII        484  GTCAC                ^GT[ACGT]AC
MamI          171  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          171  GATC                 ^GATC
MroI           74  TCCGGA               T^CCGGA
MslI          155  CATCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        237  CCGCTG               C[AC]G^C[GT]G
NcoI          479  CCATGG               C^CATGG
NdeI           10  CATATG               CA^TATG
NdeII         171  GATC                 ^GATC
NsiI          117  ATGCAT               ATGCA^T
NspI          530  GCATGT               [GA]CATG^[CT]
SacI          339  GAGCTC               GAGCT^C
Sau3AI        171  GATC                 ^GATC
ScaI          272  AGTACT               AGT^ACT
SfaNI         116  GATGC                GCATC(5/9)
SfcI          110  CTGTAG               C^T[GA][CT]AG
StyI          479  CCATGG               C^C[AT][AT]GG
TaqI          210  TCGA                 T^CGA
TfiI          396  GATTC                G^A[AT]TC
Tsp45I        484  GTCAC                ^GT[GC]AC
Tsp509I       442  AATT                 ^AATT
TthHB8I       210  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          237  CCGC                 CCGC(-3/-1)
              528  CCGC                
AvaII           6  GGTCC                G^G[AT]CC
              474  GGACC               
BanII         339  GAGCTC               G[GA]GC[CT]^C
              352  GAGCCC              
BmyI          339  GAGCTC               G[AGT]GC[ACT]^C
              352  GAGCCC              
BsmFI         454  GGGAC                GGGAC(10/14)
              492  GTCCC               
Bsp1286I      339  GAGCTC               G[AGT]GC[ACT]^C
              352  GAGCCC              
BsrI          275  ACTGG                ACTGG(1/-1)
              288  CCAGT               
DdeI          129  CTCAG                C^T[ACGT]AG
              518  CTGAG               
DraII         364  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              413  AGGCCCT             
EcoO109I      364  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              413  AGGCCCT             
HapII          75  CCGG                 C^CGG
              349  CCGG                
HpaII          75  CCGG                 C^CGG
              349  CCGG                
MboII         100  GAAGA                GAAGA(8/7)
              567  TCTTC               
MspI           75  CCGG                 C^CGG
              349  CCGG                
MwoI           83  GCCACTTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              119  GCATTTCAGGC         
PleI          388  GACTC                GAGTC(4/5)
              581  GACTC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AfaI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstF5I      BstNI       Cfr13I      
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MnlI        MvaI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       TseI        
TspRI       
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