Restriction map for pF1KB7207
 (Linear) (Six-base) MAPSORT of: KIBB7207.seq  Check: 9473  from: 1  to: 669



KIBB7207.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     281     669 
   Size:        281     388
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     434     669 
   Size:        254     180     235

  Fragments arranged by size: 

                 254     235     180
 
AseI AT'TA_AT

Cuts at:      0     599     669 
   Size:        599      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318     669 
   Size:        318     351
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     290     669 
   Size:        290     379
 
BmgBI CAC'GTC

Cuts at:      0      25     669 
   Size:         25     644
 
BseYI C'CCAG_C

Cuts at:      0       4     669 
   Size:          4     665
 
BsmI GAATG_Cn'

Cuts at:      0     215     669 
   Size:        215     454
 
BstZ17I GTA'TAC

Cuts at:      0     282     669 
   Size:        282     387
 
EarI CTCTTCn'nnn_

Cuts at:      0      12     479     566     575     669 
   Size:         12     467      87       9      94

  Fragments arranged by size: 

                 467      94      87      12       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     434     669 
   Size:        254     180     235

  Fragments arranged by size: 

                 254     235     180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     230     669 
   Size:        230     439
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     669 
   Size:         36     633
 
PsiI TTA'TAA

Cuts at:      0     128     669 
   Size:        128     541
 
SapI GCTCTTCn'nnn_

Cuts at:      0     566     669 
   Size:        566     103
 
SspI AAT'ATT

Cuts at:      0      45     669 
   Size:         45     624
 

 Enzymes that do cut:

     AccI     AcuI     AseI     BbsI    BciVI    BmgBI    BseYI     BsmI
  BstZ17I     EarI  Eco57MI     FalI     MmeI     PsiI     SapI     SspI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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