Restriction map for pF1KB7205
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BbeI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsgI        BsiWI       BspDI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MseI        MunI        MvnI        NarI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Alw44I        421  GTGCAC               G^TGCAC
AocI          252  CCTGAGG              CC^T[ACGT]AGG
ApaLI         421  GTGCAC               G^TGCAC
AspHI         421  GTGCAC               G[AT]GC[AT]^C
BalI          187  TGGCCA               TGG^CCA
BamHI         779  GGATCC               G^GATCC
BbrPI         733  CACGTG               CAC^GTG
BbsI          656  GTCTTC               GAAGAC(2/6)
BcgI          413  CGATCCCGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1075  CTAG                 C^TAG
BglII         707  AGATCT               A^GATCT
BpuAI         656  GTCTTC               GAAGAC(2/6)
BsaAI         733  CACGTG               [CT]AC^GT[GA]
BsiEI         126  CGACCG               CG[GA][CT]^CG
BsiHKAI       421  GTGCAC               G[AT]GC[AT]^C
BsmBI         996  CGTCTC               CGTCTC(1/5)
BsmI          789  GAATGC               GAATGC(1/-1)
Bsp1407I      305  TGTACA               T^GTACA
BspHI         861  TCATGA               T^CATGA
BspMI        1028  ACCTGC               ACCTGC(4/8)
BsrDI        1013  GCAATG               GCAATG(2/0)
BsrGI         305  TGTACA               T^GTACA
BsrI         1168  CCAGT                ACTGG(1/-1)
BstAPI        423  GCACACGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          24  CCACCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        252  CCTGAGG              CC^T[ACGT]AGG
EarI         1003  CTCTTC               CTCTTC(1/4)
Eco81I        252  CCTGAGG              CC^T[ACGT]AGG
FseI            3  GGCCGGCC             GGCCGG^CC
HaeII         821  AGCGCC               [GA]GCGC^[CT]
HphI          992  TCACC                GGTGA(8/7)
Ksp632I      1003  CTCTTC               CTCTTC(1/4)
MaeI         1075  CTAG                 C^TAG
MaeII         734  ACGT                 A^CGT
MaeIII        991  GTCAC                ^GT[ACGT]AC
MluNI         187  TGGCCA               TGG^CCA
MscI          187  TGGCCA               TGG^CCA
MslI          630  CACCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI            4  GCCGGC               GCC^GGC
NdeI          923  CATATG               CA^TATG
NgoMIV          4  GCCGGC               G^CCGGC
PmaCI         733  CACGTG               CAC^GTG
PmlI          733  CACGTG               CAC^GTG
PpuMI          87  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI          861  TCATGA               T^CATGA
SfcI         1193  CTGTAG               C^T[GA][CT]AG
SmlI          838  CTCAAG               C^T[CT][GA]AG
SspBI         305  TGTACA               T^GTACA
TfiI          966  GAATC                G^A[AT]TC
Tsp45I        991  GTCAC                ^GT[GC]AC
XcmI          661  CCACTACCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII        282  TCCGGA               T^CCGGA
              950  TCCGGA              
AlwNI         229  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              820  CAGCGCCTG           
AviII         467  TGCGCA               TGC^GCA
              605  TGCGCA              
BbvCI         439  GCTGAGG              CCTCAGC(-5/-2)
              453  GCTGAGG             
BglI          133  GCCCCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              505  GCCACCGAGGC         
BsaI         1242  GAGACC               GGTCTC(1/5)
             1125  GGTCTC              
BsaWI         282  TCCGGA               [AT]^CCGG[AT]
              950  TCCGGA              
BseAI         282  TCCGGA               T^CCGGA
              950  TCCGGA              
BseRI         255  GAGGAG               GAGGAG(10/8)
              298  GAGGAG              
BsmFI         579  GGGAC                GGGAC(10/14)
              476  GTCCC               
BspEI         282  TCCGGA               T^CCGGA
              950  TCCGGA              
BsrFI           4  GCCGGC               [GA]^CCGG[CT]
              290  ACCGGC              
BssSI         191  CACGAG               CACGAG(-5/-1)
              487  CACGAG              
BstF5I        309  CATCC                GGATG(2/0)
             1155  CATCC               
BstYI         707  AGATCT               [GA]^GATC[CT]
              779  GGATCC              
Cfr10I          4  GCCGGC               [GA]^CCGG[CT]
              290  ACCGGC              
DraIII        180  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              424  CACACGGTG           
FokI          309  CATCC                GGATG(9/13)
             1155  CATCC               
FspI          467  TGCGCA               TGC^GCA
              605  TGCGCA              
MboII         657  TCTTC                GAAGA(8/7)
             1004  TCTTC               
MflI          707  AGATCT               [GA]^GATC[CT]
              779  GGATCC              
MroI          282  TCCGGA               T^CCGGA
              950  TCCGGA              
SfiI          132  GGCCCCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              504  GGCCACCGAGGCC       
StuI          195  AGGCCT               AGG^CCT
              269  AGGCCT              
TspRI         602  CACTG                CA[GC]TG[ACGT][ACGT]^
             1169  CAGTG               
XhoII         707  AGATCT               [GA]^GATC[CT]
              779  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MboI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SmaI        
StyI        TseI        Van91I      XmaCI       XmaI        
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