Restriction map for pF1KB7205
 (Linear) (Six-base) MAPSORT of: KIBB7205.seq  Check: 9045  from: 1  to: 1278



KIBB7205.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1037    1278 
   Size:       1037     241
 
AlwNI CAG_nnn'CTG

Cuts at:      0     234     825    1278 
   Size:        234     591     453

  Fragments arranged by size: 

                 591     453     234
 
ApaI G_GGCC'C

Cuts at:      0      78     135     742     894    1273    1278 
   Size:         78      57     607     152     379       5

  Fragments arranged by size: 

                 607     379     152      78      57       5
 
ApaLI G'TGCA_C

Cuts at:      0     421    1278 
   Size:        421     857
 
AvaI C'yCGr_G

Cuts at:      0      71     373     394     746     812    1051    1278 
   Size:         71     302      21     352      66     239     227

  Fragments arranged by size: 

                 352     302     239     227      71      66      21
 
BamHI G'GATC_C

Cuts at:      0     779    1278 
   Size:        779     499
 
BanI G'GyrC_C

Cuts at:      0     429    1055    1173    1250    1278 
   Size:        429     626     118      77      28

  Fragments arranged by size: 

                 626     429     118      77      28
 
BanII G_rGCy'C

Cuts at:      0      78     135     394     732     742     894    1273    1278 
   Size:         78      57     259     338      10     152     379       5

  Fragments arranged by size: 

                 379     338     259     152      78      57      10       5
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     649    1278 
   Size:        649     629
 
BbvCI CC'TCA_GC

Cuts at:      0     440     454    1278 
   Size:        440      14     824

  Fragments arranged by size: 

                 824     440      14
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     402     436    1278 
   Size:        402      34     842

  Fragments arranged by size: 

                 842     402      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     139     511    1278 
   Size:        139     372     767

  Fragments arranged by size: 

                 767     372     139
 
BglII A'GATC_T

Cuts at:      0     707    1278 
   Size:        707     571
 
Bme1580I G_kGCm'C

Cuts at:      0      78     135     348     425     434     742     894    1060 
   Size:         78      57     213      77       9     308     152     166

Cuts at:   1060    1178    1273    1278 
   Size:        118      95       5

  Fragments arranged by size: 

                 308     213     166     152     118      95      78      77
                  57       9       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     192    1278 
   Size:        192    1086
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     358     368     426     556     586     868    1151 
   Size:        256     102      10      58     130      30     282     283

Cuts at:   1151    1260    1278 
   Size:        109      18

  Fragments arranged by size: 

                 283     282     256     130     109     102      58      30
                  18      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     440     454    1278 
   Size:        440      14     824

  Fragments arranged by size: 

                 824     440      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     823    1278 
   Size:        823     455
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1131    1236    1278 
   Size:       1131     105      42

  Fragments arranged by size: 

                1131     105      42
 
BsaAI yAC'GTr

Cuts at:      0     735    1278 
   Size:        735     543
 
BsaWI w'CCGG_w

Cuts at:      0     282     950    1278 
   Size:        282     668     328

  Fragments arranged by size: 

                 668     328     282
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     289     319     719     749     974    1004    1278 
   Size:        289      30     400      30     225      30     274

  Fragments arranged by size: 

                 400     289     274     225      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     270     313    1278 
   Size:        270      43     965

  Fragments arranged by size: 

                 965     270      43
 
BseYI C'CCAG_C

Cuts at:      0     240     764    1161    1224    1278 
   Size:        240     524     397      63      54

  Fragments arranged by size: 

                 524     397     240      63      54
 
BsiEI CG_ry'CG

Cuts at:      0     129    1278 
   Size:        129    1149
 
BsiHKAI G_wGCw'C

Cuts at:      0     425    1278 
   Size:        425     853
 
BsmI GAATG_Cn'

Cuts at:      0     795    1278 
   Size:        795     483
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1002    1278 
   Size:       1002     276
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     135     348     394     425     434     732     742 
   Size:         78      57     213      46      31       9     298      10

Cuts at:    742     894    1060    1178    1273    1278 
   Size:        152     166     118      95       5

  Fragments arranged by size: 

                 298     213     166     152     118      95      78      57
                  46      31      10       9       5
 
BspEI T'CCGG_A

Cuts at:      0     282     950    1278 
   Size:        282     668     328

  Fragments arranged by size: 

                 668     328     282
 
BspHI T'CATG_A

Cuts at:      0     861    1278 
   Size:        861     417
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1037    1278 
   Size:       1037     241
 
BsrDI GCAATG_nn'

Cuts at:      0    1020    1278 
   Size:       1020     258
 
BsrFI r'CCGG_y

Cuts at:      0       4     290    1278 
   Size:          4     286     988

  Fragments arranged by size: 

                 988     286       4
 
BsrGI T'GTAC_A

Cuts at:      0     305    1278 
   Size:        305     973
 
BssSI C'ACGA_G

Cuts at:      0     191     487    1278 
   Size:        191     296     791

  Fragments arranged by size: 

                 791     296     191
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     429    1278 
   Size:        429     849
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31    1278 
   Size:         31    1247
 
BstYI r'GATC_y

Cuts at:      0     707     779    1278 
   Size:        707      72     499

  Fragments arranged by size: 

                 707     499      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     253    1278 
   Size:        253    1025
 
BtgI C'CryG_G

Cuts at:      0     120     152     212     434     651    1119    1134    1278 
   Size:        120      32      60     222     217     468      15     144

  Fragments arranged by size: 

                 468     222     217     144     120      60      32      15
            
 
BtsI GCAGTG_nn'

Cuts at:      0     601    1278 
   Size:        601     677
 
DraIII CAC_nnn'GTG

Cuts at:      0     185     429    1278 
   Size:        185     244     849

  Fragments arranged by size: 

                 849     244     185
 
EaeI y'GGCC_r

Cuts at:      0       2     187    1184    1278 
   Size:          2     185     997      94

  Fragments arranged by size: 

                 997     185      94       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1009    1278 
   Size:       1009     269
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     106     214    1011    1278 
   Size:        106     108     797     267

  Fragments arranged by size: 

                 797     267     108     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     358     368     426     556     586     868    1151 
   Size:        256     102      10      58     130      30     282     283

Cuts at:   1151    1260    1278 
   Size:        109      18

  Fragments arranged by size: 

                 283     282     256     130     109     102      58      30
                  18      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75      88     132     531     890     891    1269    1270 
   Size:         75      13      44     399     359       1     378       1

Cuts at:   1270    1278 
   Size:          8

  Fragments arranged by size: 

                 399     378     359      75      44      13       8       1
                   1
 
FseI GG_CCGG'CC

Cuts at:      0       8    1278 
   Size:          8    1270
 
FspI TGC'GCA

Cuts at:      0     469     607    1278 
   Size:        469     138     671

  Fragments arranged by size: 

                 671     469     138
 
HaeII r_GCGC'y

Cuts at:      0     825    1278 
   Size:        825     453
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     974    1006    1278 
   Size:        974      32     272

  Fragments arranged by size: 

                 974     272      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     573    1278 
   Size:        573     705
 
MscI TGG'CCA

Cuts at:      0     189    1278 
   Size:        189    1089
 
MslI CAynn'nnrTG

Cuts at:      0     634    1278 
   Size:        634     644
 
MspA1I CmG'CkG

Cuts at:      0     138     207     234     404     473     768    1092    1278 
   Size:        138      69      27     170      69     295     324     186

  Fragments arranged by size: 

                 324     295     186     170     138      69      69      27
            
 
NaeI GCC'GGC

Cuts at:      0       6    1278 
   Size:          6    1272
 
NcoI C'CATG_G

Cuts at:      0     120     152    1119    1134    1278 
   Size:        120      32     967      15     144

  Fragments arranged by size: 

                 967     144     120      32      15
 
NdeI CA'TA_TG

Cuts at:      0     924    1278 
   Size:        924     354
 
NgoMIV G'CCGG_C

Cuts at:      0       4    1278 
   Size:          4    1274
 
PasI CC'CwG_GG

Cuts at:      0     894    1278 
   Size:        894     384
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     185     234     825    1278 
   Size:        185      49     591     453

  Fragments arranged by size: 

                 591     453     185      49
 
PmlI CAC'GTG

Cuts at:      0     735    1278 
   Size:        735     543
 
PpuMI rG'GwC_Cy

Cuts at:      0      88    1278 
   Size:         88    1190
 
PspOMI G'GGCC_C

Cuts at:      0      74     131     738     890    1269    1278 
   Size:         74      57     607     152     379       9

  Fragments arranged by size: 

                 607     379     152      74      57       9
 
PvuII CAG'CTG

Cuts at:      0     207     234     473     768    1092    1278 
   Size:        207      27     239     295     324     186

  Fragments arranged by size: 

                 324     295     239     207     186      27
 
SfcI C'TryA_G

Cuts at:      0    1193    1278 
   Size:       1193      85
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     139     511    1278 
   Size:        139     372     767

  Fragments arranged by size: 

                 767     372     139
 
SmaI CCC'GGG

Cuts at:      0      73     748     814    1053    1278 
   Size:         73     675      66     239     225

  Fragments arranged by size: 

                 675     239     225      73      66
 
SmlI C'TyrA_G

Cuts at:      0     838    1278 
   Size:        838     440
 
StuI AGG'CCT

Cuts at:      0     197     271    1278 
   Size:        197      74    1007

  Fragments arranged by size: 

                1007     197      74
 
StyI C'CwwG_G

Cuts at:      0     120     152    1119    1134    1262    1278 
   Size:        120      32     967      15     128      16

  Fragments arranged by size: 

                 967     128     120      32      16      15
 
TatI w'GTAC_w

Cuts at:      0     305     851    1278 
   Size:        305     546     427

  Fragments arranged by size: 

                 546     427     305
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112     719     751    1278 
   Size:         80      32     607      32     527

  Fragments arranged by size: 

                 607     527      80      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     668    1278 
   Size:        668     610
 
XmaI C'CCGG_G

Cuts at:      0      71     746     812    1051    1278 
   Size:         71     675      66     239     227

  Fragments arranged by size: 

                 675     239     227      71      66
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I
     FseI     FspI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     PasI    PflMI     PmlI    PpuMI
   PspOMI    PvuII     SfcI     SfiI     SmaI     SmlI     StuI     StyI
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI     BlpI
    BmgBI     BmrI     BmtI    BsaBI    BsaHI     BsgI    BsiWI    BsrBI
   BssHII    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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