Restriction map for pF1KB7204
##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          319  AAATTC               [GA]^AATT[CT]
AluI           43  AGCT                 AG^CT
AlwNI         340  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          319  AAATTC               [GA]^AATT[CT]
AspHI          37  GTGCTC               G[AT]GC[AT]^C
BbvI          292  GCTGC                GCAGC(8/12)
BlpI           39  GCTCAGC              GC^T[ACGT]AGC
BmyI           37  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I       39  GCTCAGC              GC^T[ACGT]AGC
BsaI          304  GAGACC               GGTCTC(1/5)
BsiHKAI        37  GTGCTC               G[AT]GC[AT]^C
BsiYI         183  CCCCCAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          183  CCCCCAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         304  GAGAC                GTCTC(1/5)
Bsp1286I       37  GTGCTC               G[AGT]GC[ACT]^C
BspHI         512  TCATGA               T^CATGA
BsrI          207  CCAGT                ACTGG(1/-1)
BstYI         517  AGATCC               [GA]^GATC[CT]
CelII          39  GCTCAGC              GC^T[ACGT]AGC
DraII         106  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI           26  TGGCCG               [CT]^GGCC[GA]
EcoO109I      106  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Fnu4HI        292  GCTGC                GC^[ACGT]GC
ItaI          292  GCTGC                GC^[ACGT]GC
MaeIII        367  GTGAC                ^GT[ACGT]AC
MflI          517  AGATCC               [GA]^GATC[CT]
MseI          553  TTAA                 T^TAA
MwoI          226  GCCATCTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI          512  TCATGA               T^CATGA
SmlI          221  CTCAAG               C^T[CT][GA]AG
Tru9I         553  TTAA                 T^TAA
TseI          292  GCTGC                G^C[AT]GC
Tsp45I        367  GTGAC                ^GT[GC]AC
Tsp509I       320  AATT                 ^AATT
XcmI           53  CCATCTGTTCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         517  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI          249  GATCC                GGATC(4/5)
              518  GATCC               
BfaI          313  CTAG                 C^TAG
              355  CTAG                
BstF5I        417  CATCC                GGATG(2/0)
              300  GGATG               
Cfr13I          3  GGCCC                G^G[ACGT]CC
              107  GGGCC               
DdeI           40  CTCAG                C^T[ACGT]AG
               80  CTCAG               
EarI          268  CTCTTC               CTCTTC(1/4)
              388  GAAGAG              
FokI          417  CATCC                GGATG(9/13)
              300  GGATG               
Ksp632I       268  CTCTTC               CTCTTC(1/4)
              388  GAAGAG              
MaeI          313  CTAG                 C^TAG
              355  CTAG                
NlaIII        241  CATG                 CATG^
              513  CATG                
PleI           84  GACTC                GAGTC(4/5)
              412  GACTC               
Sau96I          3  GGCCC                G^G[ACGT]CC
              107  GGGCC               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

BsaJI       BssKI       BstNI       DpnII       EcoRII      
HaeIII      HinfI       MboI        MboII       MnlI        
MvaI        NdeII       Sau3AI      ScrFI       TfiI        
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