Restriction map for pF1KB7199
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        Aor51HI     
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BamHI       BbsI        BbvCI       BciVI       
BclI        BfrI        BlnI        BpuAI       BsaBI       
BsaI        BseAI       BsgI        BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspMI       BsrBI       BsrDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI         1081  GAATTT               [GA]^AATT[CT]
AcyI          709  GGCGCC               G[GA]^CG[CT]C
AflIII        840  ACATGT               A^C[GA][CT]GT
Alw44I        961  GTGCAC               G^TGCAC
AlwNI         745  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         961  GTGCAC               G^TGCAC
ApoI         1081  GAATTT               [GA]^AATT[CT]
AscI           58  GGCGCGCC             GG^CGCGCC
AviII        1208  TGCGCA               TGC^GCA
BalI          605  TGGCCA               TGG^CCA
BbeI          709  GGCGCC               GGCGC^C
BbrPI         532  CACGTG               CAC^GTG
BglII        1010  AGATCT               A^GATCT
BpmI          446  CTCCAG               CTGGAG(16/14)
BsaAI         532  CACGTG               [CT]AC^GT[GA]
BsaHI         709  GGCGCC               G[GA]^CG[CT]C
BsaWI         806  ACCGGA               [AT]^CCGG[AT]
BseRI         898  GAGGAG               GAGGAG(10/8)
BsmFI         122  GGGAC                GGGAC(10/14)
BspHI        1227  TCATGA               T^CATGA
BspLU11I      840  ACATGT               A^CATGT
BssHII         59  GCGCGC               G^CGCGC
BssSI          73  CACGAG               CACGAG(-5/-1)
BstAPI       1169  GCAAAGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT14I       848  CCATGG               C^C[AT][AT]GG
FspI         1208  TGCGCA               TGC^GCA
HgaI         1100  GACGC                GACGC(5/10)
Hin1I         709  GGCGCC               G[GA]^CG[CT]C
HincII        946  GTCAAC               GT[CT]^[GA]AC
HindII        946  GTCAAC               GT[CT]^[GA]AC
KasI          709  GGCGCC               G^GCGCC
KspI          182  CCGCGG               CCGC^GG
MluNI         605  TGGCCA               TGG^CCA
MscI          605  TGGCCA               TGG^CCA
MseI         1385  TTAA                 T^TAA
NarI          709  GGCGCC               GG^CGCC
NcoI          848  CCATGG               C^CATGG
PleI          124  GACTC                GAGTC(4/5)
PmaCI         532  CACGTG               CAC^GTG
PmlI          532  CACGTG               CAC^GTG
PpuMI        1048  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         837  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1337  CTGCAG               CTGCA^G
RcaI         1227  TCATGA               T^CATGA
SacII         182  CCGCGG               CCGC^GG
SexAI         545  ACCAGGT              A^CC[AT]GGT
SfoI          709  GGCGCC               GGC^GCC
SphI          441  GCATGC               GCATG^C
StyI          848  CCATGG               C^C[AT][AT]GG
TaqI          767  TCGA                 T^CGA
Tru9I        1385  TTAA                 T^TAA
TthHB8I       767  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          936  GTAC                 GT^AC
             1395  GTAC                
AlwI         1038  GATCC                GGATC(4/5)
              764  GGATC               
ApaI          703  GGGCCC               GGGCC^C
              864  GGGCCC              
BfaI          470  CTAG                 C^TAG
             1349  CTAG                
BglI          421  GCCGGCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              522  GCCCACAGGGC         
BstF5I        456  CATCC                GGATG(2/0)
              954  CATCC               
BstXI         245  CCAACGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1359  CCACAACCCTGG        
BstYI        1010  AGATCT               [GA]^GATC[CT]
             1037  AGATCC              
DraII         863  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1048  AGGACCC             
EagI          579  CGGCCG               C^GGCCG
              632  CGGCCG              
EarI          415  CTCTTC               CTCTTC(1/4)
              795  GAAGAG              
EclXI         579  CGGCCG               C^GGCCG
              632  CGGCCG              
Eco52I        579  CGGCCG               C^GGCCG
              632  CGGCCG              
EcoO109I      863  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1048  AGGACCC             
EcoT22I       823  ATGCAT               ATGCA^T
             1135  ATGCAT              
FokI          456  CATCC                GGATG(9/13)
              954  CATCC               
Ksp632I       415  CTCTTC               CTCTTC(1/4)
              795  GAAGAG              
MaeI          470  CTAG                 C^TAG
             1349  CTAG                
MflI         1010  AGATCT               [GA]^GATC[CT]
             1037  AGATCC              
NsiI          823  ATGCAT               ATGCA^T
             1135  ATGCAT              
PvuII         449  CAGCTG               CAG^CTG
              778  CAGCTG              
RsaI          936  GTAC                 GT^AC
             1395  GTAC                
SacI          210  GAGCTC               GAGCT^C
             1200  GAGCTC              
SapI          795  GAAGAGC              GCTCTTC(1/4)
              414  GCTCTTC             
SmlI         1033  CTCAAG               C^T[CT][GA]AG
             1235  CTCAAG              
Tsp509I      1082  AATT                 ^AATT
             1381  AATT                
TspRI         381  CAGTG                CA[GC]TG[ACGT][ACGT]^
              602  CAGTG               
XhoII        1010  AGATCT               [GA]^GATC[CT]
             1037  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AluI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcgI        BcnI        BlpI        BmyI        Bpu1102I    
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EaeI        EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        TfiI        TseI        Tsp45I      
XcmI        XmaCI       XmaI        XmnI        
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