Restriction map for pF1KB7199
 (Linear) (Six-base) MAPSORT of: KIBB7199.seq  Check: 3592  from: 1  to: 1401



KIBB7199.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     798    1401 
   Size:        185     613     603

  Fragments arranged by size: 

                 613     603     185
 
AflIII A'CryG_T

Cuts at:      0     840    1401 
   Size:        840     561
 
AleI CACnn'nnGTG

Cuts at:      0    1058    1401 
   Size:       1058     343
 
AlwNI CAG_nnn'CTG

Cuts at:      0     750    1401 
   Size:        750     651
 
ApaI G_GGCC'C

Cuts at:      0     707     868    1401 
   Size:        707     161     533

  Fragments arranged by size: 

                 707     533     161
 
ApaLI G'TGCA_C

Cuts at:      0     961    1401 
   Size:        961     440
 
ApoI r'AATT_y

Cuts at:      0    1081    1401 
   Size:       1081     320
 
AscI GG'CGCG_CC

Cuts at:      0      59    1401 
   Size:         59    1342
 
AvaI C'yCGr_G

Cuts at:      0     393     460     487     908     996    1197    1401 
   Size:        393      67      27     421      88     201     204

  Fragments arranged by size: 

                 421     393     204     201      88      67      27
 
BanI G'GyrC_C

Cuts at:      0     300     512     709    1401 
   Size:        300     212     197     692

  Fragments arranged by size: 

                 692     300     212     197
 
BanII G_rGCy'C

Cuts at:      0     178     214     380     416     467     707     868    1204 
   Size:        178      36     166      36      51     240     161     336

Cuts at:   1204    1401 
   Size:        197

  Fragments arranged by size: 

                 336     240     197     178     166     161      51      36
                  36
 
BbeI G_GCGC'C

Cuts at:      0     713    1401 
   Size:        713     688
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     420     454     747     781    1188    1222    1401 
   Size:        420      34     293      34     407      34     179

  Fragments arranged by size: 

                 420     407     293     179      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0     825    1137    1401 
   Size:        825     312     264

  Fragments arranged by size: 

                 825     312     264
 
BglI GCCn_nnn'nGGC

Cuts at:      0     427     528    1401 
   Size:        427     101     873

  Fragments arranged by size: 

                 873     427     101
 
BglII A'GATC_T

Cuts at:      0    1010    1401 
   Size:       1010     391
 
BlpI GC'TnA_GC

Cuts at:      0     172     776     781    1401 
   Size:        172     604       5     620

  Fragments arranged by size: 

                 620     604     172       5
 
Bme1580I G_kGCm'C

Cuts at:      0     305     515     533     707     868     907     965    1401 
   Size:        305     210      18     174     161      39      58     436

  Fragments arranged by size: 

                 436     305     210     174     161      58      39      18
            
 
BmgBI CAC'GTC

Cuts at:      0     885    1401 
   Size:        885     516
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     595     903    1401 
   Size:        595     308     498

  Fragments arranged by size: 

                 595     498     308
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     408    1401 
   Size:        408     993
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431    1401 
   Size:        431     970
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1018    1220    1401 
   Size:       1018     202     181

  Fragments arranged by size: 

                1018     202     181
 
BsaAI yAC'GTr

Cuts at:      0     534    1401 
   Size:        534     867
 
BsaHI Gr'CG_yC

Cuts at:      0     710    1401 
   Size:        710     691
 
BsaWI w'CCGG_w

Cuts at:      0     806    1401 
   Size:        806     595
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     671     701     739     769     904     934    1186    1216 
   Size:        671      30      38      30     135      30     252      30

Cuts at:   1216    1401 
   Size:        185

  Fragments arranged by size: 

                 671     252     185     135      38      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     913    1401 
   Size:        913     488
 
BseYI C'CCAG_C

Cuts at:      0      39     304     508    1401 
   Size:         39     265     204     893

  Fragments arranged by size: 

                 893     265     204      39
 
BsiEI CG_ry'CG

Cuts at:      0     582     635     807    1122    1401 
   Size:        582      53     172     315     279

  Fragments arranged by size: 

                 582     315     279     172      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     214     965    1204    1401 
   Size:        214     751     239     197

  Fragments arranged by size: 

                 751     239     214     197
 
Bsp1286I G_dGCh'C

Cuts at:      0     178     214     305     380     416     467     515     533 
   Size:        178      36      91      75      36      51      48      18

Cuts at:    533     707     868     907     965    1204    1401 
   Size:        174     161      39      58     239     197

  Fragments arranged by size: 

                 239     197     178     174     161      91      75      58
                  51      48      39      36      36      18
 
BspHI T'CATG_A

Cuts at:      0    1227    1401 
   Size:       1227     174
 
BsrFI r'CCGG_y

Cuts at:      0      93     421     634     785    1401 
   Size:         93     328     213     151     616

  Fragments arranged by size: 

                 616     328     213     151      93
 
BssHII G'CGCG_C

Cuts at:      0      59    1401 
   Size:         59    1342
 
BssSI C'ACGA_G

Cuts at:      0      73    1401 
   Size:         73    1328
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1175    1401 
   Size:       1175     226
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     252    1366    1401 
   Size:        252    1114      35

  Fragments arranged by size: 

                1114     252      35
 
BstYI r'GATC_y

Cuts at:      0    1010    1037    1401 
   Size:       1010      27     364

  Fragments arranged by size: 

                1010     364      27
 
BtgI C'CryG_G

Cuts at:      0     182     699     848    1401 
   Size:        182     517     149     553

  Fragments arranged by size: 

                 553     517     182     149
 
EaeI y'GGCC_r

Cuts at:      0     579     605     632    1401 
   Size:        579      26      27     769

  Fragments arranged by size: 

                 769     579      27      26
 
EagI C'GGCC_G

Cuts at:      0     579     632    1401 
   Size:        579      53     769

  Fragments arranged by size: 

                 769     579      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     421     790    1401 
   Size:        421     369     611

  Fragments arranged by size: 

                 611     421     369
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     349    1401 
   Size:        349    1052
 
EcoICRI GAG'CTC

Cuts at:      0     212    1202    1401 
   Size:        212     990     199

  Fragments arranged by size: 

                 990     212     199
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     431     798    1401 
   Size:        185     246     367     603

  Fragments arranged by size: 

                 603     367     246     185
 
EcoO109I rG'GnC_Cy

Cuts at:      0     864    1049    1401 
   Size:        864     185     352

  Fragments arranged by size: 

                 864     352     185
 
FspI TGC'GCA

Cuts at:      0    1210    1401 
   Size:       1210     191
 
HaeII r_GCGC'y

Cuts at:      0     320     509     641     713    1401 
   Size:        320     189     132      72     688

  Fragments arranged by size: 

                 688     320     189     132      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     904     936    1401 
   Size:        904      32     465

  Fragments arranged by size: 

                 904     465      32
 
HincII GTy'rAC

Cuts at:      0     948    1401 
   Size:        948     453
 
KasI G'GCGC_C

Cuts at:      0     709    1401 
   Size:        709     692
 
MscI TGG'CCA

Cuts at:      0     607    1401 
   Size:        607     794
 
MslI CAynn'nnrTG

Cuts at:      0     667     673     679     958    1058    1136    1138    1401 
   Size:        667       6       6     279     100      78       2     263

  Fragments arranged by size: 

                 667     279     263     100      78       6       6       2
            
 
MspA1I CmG'CkG

Cuts at:      0     160     184     451     780    1401 
   Size:        160      24     267     329     621

  Fragments arranged by size: 

                 621     329     267     160      24
 
NaeI GCC'GGC

Cuts at:      0      95     423     636     787    1401 
   Size:         95     328     213     151     614

  Fragments arranged by size: 

                 614     328     213     151      95
 
NarI GG'CG_CC

Cuts at:      0     710    1401 
   Size:        710     691
 
NcoI C'CATG_G

Cuts at:      0     848    1401 
   Size:        848     553
 
NgoMIV G'CCGG_C

Cuts at:      0      93     421     634     785    1401 
   Size:         93     328     213     151     616

  Fragments arranged by size: 

                 616     328     213     151      93
 
NsiI A_TGCA'T

Cuts at:      0     827    1139    1401 
   Size:        827     312     262

  Fragments arranged by size: 

                 827     312     262
 
NspI r_CATG'y

Cuts at:      0     445     844     859    1137    1141    1401 
   Size:        445     399      15     278       4     260

  Fragments arranged by size: 

                 445     399     278     260      15       4
 
PasI CC'CwG_GG

Cuts at:      0      34     252    1401 
   Size:         34     218    1149

  Fragments arranged by size: 

                1149     218      34
 
PciI A'CATG_T

Cuts at:      0     840    1401 
   Size:        840     561
 
PfoI T'CCnGG_A

Cuts at:      0      11    1401 
   Size:         11    1390
 
PmlI CAC'GTG

Cuts at:      0     534    1401 
   Size:        534     867
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372    1401 
   Size:        340      32    1029

  Fragments arranged by size: 

                1029     340      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1049    1401 
   Size:       1049     352
 
PshAI GACnn'nnGTC

Cuts at:      0     841    1401 
   Size:        841     560
 
PspOMI G'GGCC_C

Cuts at:      0     703     864    1401 
   Size:        703     161     537

  Fragments arranged by size: 

                 703     537     161
 
PstI C_TGCA'G

Cuts at:      0    1341    1401 
   Size:       1341      60
 
PvuII CAG'CTG

Cuts at:      0     451     780    1401 
   Size:        451     329     621

  Fragments arranged by size: 

                 621     451     329
 
SacI G_AGCT'C

Cuts at:      0     214    1204    1401 
   Size:        214     990     197

  Fragments arranged by size: 

                 990     214     197
 
SacII CC_GC'GG

Cuts at:      0     185    1401 
   Size:        185    1216
 
SapI GCTCTTCn'nnn_

Cuts at:      0     421     790    1401 
   Size:        421     369     611

  Fragments arranged by size: 

                 611     421     369
 
SexAI A'CCwGG_T

Cuts at:      0     545    1401 
   Size:        545     856
 
SfcI C'TryA_G

Cuts at:      0     654    1295    1337    1401 
   Size:        654     641      42      64

  Fragments arranged by size: 

                 654     641      64      42
 
SfoI GGC'GCC

Cuts at:      0     711    1401 
   Size:        711     690
 
SmaI CCC'GGG

Cuts at:      0     489     910     998    1401 
   Size:        489     421      88     403

  Fragments arranged by size: 

                 489     421     403      88
 
SmlI C'TyrA_G

Cuts at:      0    1033    1235    1401 
   Size:       1033     202     166

  Fragments arranged by size: 

                1033     202     166
 
SphI G_CATG'C

Cuts at:      0     445    1401 
   Size:        445     956
 
StyI C'CwwG_G

Cuts at:      0     848    1401 
   Size:        848     553
 
TatI w'GTAC_w

Cuts at:      0     935    1401 
   Size:        935     466
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     671     703     739     771    1186    1218    1401 
   Size:        671      32      36      32     415      32     183

  Fragments arranged by size: 

                 671     415     183      36      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     531     828     855    1401 
   Size:        531     297      27     546

  Fragments arranged by size: 

                 546     531     297      27
 
XmaI C'CCGG_G

Cuts at:      0     487     908     996    1401 
   Size:        487     421      88     405

  Fragments arranged by size: 

                 487     421     405      88
 
XmnI GAAnn'nnTTC

Cuts at:      0     115     979    1069    1401 
   Size:        115     864      90     332

  Fragments arranged by size: 

                 864     332     115      90
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AvaI     BanI    BanII     BbeI     BcgI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI     BtgI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI
    HaeII    Hin4I   HincII     KasI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NsiI     NspI     PasI     PciI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SacII     SapI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI
     StyI     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI    BciVI     BclI     BmtI   Bpu10I     BsaI    BsaBI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NdeI     NheI     NotI     NruI     PacI    PflMI     PmeI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XhoI     ZraI
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