Restriction map for pF1KB7198
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AhdI        Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BbvCI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FspI        HindIII     HpaI        KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MunI        MvnI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        260  GGTACC               G^GTACC
AcsI         1234  GAATTT               [GA]^AATT[CT]
AflIII        296  ACGTGT               A^C[GA][CT]GT
AgeI          302  ACCGGT               A^CCGGT
ApoI         1234  GAATTT               [GA]^AATT[CT]
Asp718I       260  GGTACC               G^GTACC
BamHI         156  GGATCC               G^GATCC
BbeI            7  GGCGCC               GGCGC^C
BbrPI         295  CACGTG               CAC^GTG
BbsI          580  GTCTTC               GAAGAC(2/6)
BcgI          127  CGACGCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         994  GTATCC               GTATCC(6/5)
BpuAI         580  GTCTTC               GAAGAC(2/6)
BsmAI         100  GAGAC                GTCTC(1/5)
BsmBI         100  GAGACG               CGTCTC(1/5)
BspHI        1086  TCATGA               T^CATGA
BsrDI         786  GCAATG               GCAATG(2/0)
BstXI         636  CCAGTGGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1129  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         1340  CCACGG               C^C[GA][CT]GG
EarI         1242  GAAGAG               CTCTTC(1/4)
EcoO109I     1129  GGGACCC              [GA]G^G[ACGT]CC[CT]
FseI           60  GGCCGGCC             GGCCGG^CC
HincII       1213  GTCAAC               GT[CT]^[GA]AC
HindII       1213  GTCAAC               GT[CT]^[GA]AC
KasI            7  GGCGCC               G^GCGCC
KpnI          260  GGTACC               GGTAC^C
Ksp632I      1242  GAAGAG               CTCTTC(1/4)
MslI          948  CATTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI            7  GGCGCC               GG^CGCC
NspI         1355  GCATGT               [GA]CATG^[CT]
PinAI         302  ACCGGT               A^CCGGT
PmaCI         295  CACGTG               CAC^GTG
PmlI          295  CACGTG               CAC^GTG
PpuMI        1129  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI         1086  TCATGA               T^CATGA
SacI         1159  GAGCTC               GAGCT^C
SapI         1242  GAAGAGC              GCTCTTC(1/4)
ScaI          701  AGTACT               AGT^ACT
SfoI            7  GGCGCC               GGC^GCC
SmaI         1124  CCCGGG               CCC^GGG
TaqI          197  TCGA                 T^CGA
TthHB8I       197  TCGA                 T^CGA
XmaCI        1124  CCCGGG               C^CCGGG
XmaI         1124  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI            7  GGCGCC               G[GA]^CG[CT]C
              128  GACGCC              
AlwNI         418  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1180  CAGGGGCTG           
Aor51HI       140  AGCGCT               AGC^GCT
             1253  AGCGCT              
AvaI           43  CTCGGG               C^[CT]CG[GA]G
             1124  CCCGGG              
BalI          214  TGGCCA               TGG^CCA
             1193  TGGCCA              
BanII         646  GGGCTC               G[GA]GC[CT]^C
             1159  GAGCTC              
BpmI          345  CTCCAG               CTGGAG(16/14)
              357  CTGGAG              
BsaAI         295  CACGTG               [CT]AC^GT[GA]
              805  TACGTG              
BsaHI           7  GGCGCC               G[GA]^CG[CT]C
              128  GACGCC              
BsaWI         188  ACCGGA               [AT]^CCGG[AT]
              302  ACCGGT              
BsoBI          43  CTCGGG               C^[CT]CG[GA]G
             1124  CCCGGG              
Bsp1407I      752  TGTACA               T^GTACA
             1358  TGTACA              
BspMI         263  ACCTGC               ACCTGC(4/8)
              512  ACCTGC              
BsrGI         752  TGTACA               T^GTACA
             1358  TGTACA              
BstYI         156  GGATCC               [GA]^GATC[CT]
              791  GGATCT              
Eco47III      140  AGCGCT               AGC^GCT
             1253  AGCGCT              
EcoNI         147  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              867  CCTCTATGAGG         
HgaI          128  GACGC                GACGC(5/10)
              592  GACGC               
Hin1I           7  GGCGCC               G[GA]^CG[CT]C
              128  GACGCC              
HphI          954  GGTGA                GGTGA(8/7)
             1169  TCACC               
MaeII         296  ACGT                 A^CGT
              806  ACGT                
MflI          156  GGATCC               [GA]^GATC[CT]
              791  GGATCT              
MluNI         214  TGGCCA               TGG^CCA
             1193  TGGCCA              
MscI          214  TGGCCA               TGG^CCA
             1193  TGGCCA              
NaeI            4  GCCGGC               GCC^GGC
               61  GCCGGC              
NgoMIV          4  GCCGGC               G^CCGGC
               61  GCCGGC              
PstI          331  CTGCAG               CTGCA^G
             1383  CTGCAG              
SfaNI         553  GCATC                GCATC(5/9)
              986  GCATC               
SfcI          331  CTGCAG               C^T[GA][CT]AG
             1383  CTGCAG              
SmlI         1264  CTTGAG               C^T[CT][GA]AG
             1306  CTCAAG              
SspBI         752  TGTACA               T^GTACA
             1358  TGTACA              
StuI          321  AGGCCT               AGG^CCT
             1376  AGGCCT              
Tsp509I       718  AATT                 ^AATT
             1235  AATT                
XhoII         156  GGATCC               [GA]^GATC[CT]
              791  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanI        BbvI        BcnI        BmyI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       EaeI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       TseI        
TspRI       
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