Restriction map for pF1KB7198
 (Linear) (Six-base) MAPSORT of: KIBB7198.seq  Check: 9914  from: 1  to: 1389



KIBB7198.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     521    1389 
   Size:        521     868
 
Acc65I G'GTAC_C

Cuts at:      0     260    1389 
   Size:        260    1129
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     489     622     780    1024    1389 
   Size:        338     151     133     158     244     365

  Fragments arranged by size: 

                 365     338     244     158     151     133
 
AfeI AGC'GCT

Cuts at:      0     142    1255    1389 
   Size:        142    1113     134

  Fragments arranged by size: 

                1113     142     134
 
AflIII A'CryG_T

Cuts at:      0     296    1389 
   Size:        296    1093
 
AgeI A'CCGG_T

Cuts at:      0     302    1389 
   Size:        302    1087
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423    1185    1389 
   Size:        423     762     204

  Fragments arranged by size: 

                 762     423     204
 
ApoI r'AATT_y

Cuts at:      0    1234    1389 
   Size:       1234     155
 
AvaI C'yCGr_G

Cuts at:      0      43    1124    1389 
   Size:         43    1081     265

  Fragments arranged by size: 

                1081     265      43
 
BamHI G'GATC_C

Cuts at:      0     156    1389 
   Size:        156    1233
 
BanI G'GyrC_C

Cuts at:      0       7      79     260    1389 
   Size:          7      72     181    1129

  Fragments arranged by size: 

                1129     181      72       7
 
BanII G_rGCy'C

Cuts at:      0     650    1163    1389 
   Size:        650     513     226

  Fragments arranged by size: 

                 650     513     226
 
BbeI G_GCGC'C

Cuts at:      0      11    1389 
   Size:         11    1378
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     573    1389 
   Size:        573     816
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116     150    1389 
   Size:        116      34    1239

  Fragments arranged by size: 

                1239     116      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1005    1389 
   Size:       1005     384
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     249    1389 
   Size:        249    1140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     378    1389 
   Size:        330      48    1011

  Fragments arranged by size: 

                1011     330      48
 
Bpu10I CC'TnA_GC

Cuts at:      0    1327    1389 
   Size:       1327      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    1291    1389 
   Size:       1285       6      98

  Fragments arranged by size: 

                1285      98       6
 
BsaAI yAC'GTr

Cuts at:      0     297     807    1389 
   Size:        297     510     582

  Fragments arranged by size: 

                 582     510     297
 
BsaHI Gr'CG_yC

Cuts at:      0       8     129    1389 
   Size:          8     121    1260

  Fragments arranged by size: 

                1260     121       8
 
BsaWI w'CCGG_w

Cuts at:      0     188     302    1389 
   Size:        188     114    1087

  Fragments arranged by size: 

                1087     188     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     730     760    1389 
   Size:        445      30     255      30     629

  Fragments arranged by size: 

                 629     445     255      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394     427     487     640    1389 
   Size:        394      33      60     153     749

  Fragments arranged by size: 

                 749     394     153      60      33
 
BseYI C'CCAG_C

Cuts at:      0     626     818    1077    1247    1389 
   Size:        626     192     259     170     142

  Fragments arranged by size: 

                 626     259     192     170     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     294     499     504    1389 
   Size:        127     167     205       5     885

  Fragments arranged by size: 

                 885     205     167     127       5
 
BsiHKAI G_wGCw'C

Cuts at:      0    1121    1163    1271    1389 
   Size:       1121      42     108     118

  Fragments arranged by size: 

                1121     118     108      42
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      94    1389 
   Size:         94    1295
 
Bsp1286I G_dGCh'C

Cuts at:      0     650    1121    1163    1271    1389 
   Size:        650     471      42     108     118

  Fragments arranged by size: 

                 650     471     118     108      42
 
BspHI T'CATG_A

Cuts at:      0    1086    1389 
   Size:       1086     303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     272     521    1389 
   Size:        272     249     868

  Fragments arranged by size: 

                 868     272     249
 
BsrDI GCAATG_nn'

Cuts at:      0     793    1389 
   Size:        793     596
 
BsrFI r'CCGG_y

Cuts at:      0       4      61     302    1389 
   Size:          4      57     241    1087

  Fragments arranged by size: 

                1087     241      57       4
 
BsrGI T'GTAC_A

Cuts at:      0     752    1358    1389 
   Size:        752     606      31

  Fragments arranged by size: 

                 752     606      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     643    1389 
   Size:        643     746
 
BstYI r'GATC_y

Cuts at:      0     156     791    1389 
   Size:        156     635     598

  Fragments arranged by size: 

                 635     598     156
 
BtgI C'CryG_G

Cuts at:      0    1340    1389 
   Size:       1340      49
 
BtsI GCAGTG_nn'

Cuts at:      0     285     516     757    1338    1389 
   Size:        285     231     241     581      51

  Fragments arranged by size: 

                 581     285     241     231      51
 
EaeI y'GGCC_r

Cuts at:      0       2     214     713    1193    1389 
   Size:          2     212     499     480     196

  Fragments arranged by size: 

                 499     480     212     196       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1237    1389 
   Size:       1237     152
 
EcoICRI GAG'CTC

Cuts at:      0    1161    1389 
   Size:       1161     228
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     338     378     489     622     780    1024    1389 
   Size:        330       8      40     111     133     158     244     365

  Fragments arranged by size: 

                 365     330     244     158     133     111      40       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     151     871    1389 
   Size:        151     720     518

  Fragments arranged by size: 

                 720     518     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1130    1389 
   Size:       1130     259
 
FseI GG_CCGG'CC

Cuts at:      0      65    1389 
   Size:         65    1324
 
HaeII r_GCGC'y

Cuts at:      0      11     144    1257    1389 
   Size:         11     133    1113     132

  Fragments arranged by size: 

                1113     133     132      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173    1389 
   Size:        141      32    1216

  Fragments arranged by size: 

                1216     141      32
 
HincII GTy'rAC

Cuts at:      0    1215    1389 
   Size:       1215     174
 
KasI G'GCGC_C

Cuts at:      0       7    1389 
   Size:          7    1382
 
KpnI G_GTAC'C

Cuts at:      0     264    1389 
   Size:        264    1125
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     926    1389 
   Size:        350     576     463

  Fragments arranged by size: 

                 576     463     350
 
MscI TGG'CCA

Cuts at:      0     216    1195    1389 
   Size:        216     979     194

  Fragments arranged by size: 

                 979     216     194
 
MslI CAynn'nnrTG

Cuts at:      0     952    1389 
   Size:        952     437
 
MspA1I CmG'CkG

Cuts at:      0      21     251     316    1230    1389 
   Size:         21     230      65     914     159

  Fragments arranged by size: 

                 914     230     159      65      21
 
NaeI GCC'GGC

Cuts at:      0       6      63    1389 
   Size:          6      57    1326

  Fragments arranged by size: 

                1326      57       6
 
NarI GG'CG_CC

Cuts at:      0       8    1389 
   Size:          8    1381
 
NgoMIV G'CCGG_C

Cuts at:      0       4      61    1389 
   Size:          4      57    1328

  Fragments arranged by size: 

                1328      57       4
 
NspI r_CATG'y

Cuts at:      0    1359    1389 
   Size:       1359      30
 
PasI CC'CwG_GG

Cuts at:      0      75     269     848    1389 
   Size:         75     194     579     541

  Fragments arranged by size: 

                 579     541     194      75
 
PmlI CAC'GTG

Cuts at:      0     297    1389 
   Size:        297    1092
 
PpuMI rG'GwC_Cy

Cuts at:      0    1130    1389 
   Size:       1130     259
 
PstI C_TGCA'G

Cuts at:      0     335    1387    1389 
   Size:        335    1052       2

  Fragments arranged by size: 

                1052     335       2
 
PvuII CAG'CTG

Cuts at:      0     251     316    1230    1389 
   Size:        251      65     914     159

  Fragments arranged by size: 

                 914     251     159      65
 
SacI G_AGCT'C

Cuts at:      0    1163    1389 
   Size:       1163     226
 
SanDI GG'GwC_CC

Cuts at:      0    1130    1389 
   Size:       1130     259
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1237    1389 
   Size:       1237     152
 
ScaI AGT'ACT

Cuts at:      0     703    1389 
   Size:        703     686
 
SfcI C'TryA_G

Cuts at:      0     331    1383    1389 
   Size:        331    1052       6

  Fragments arranged by size: 

                1052     331       6
 
SfoI GGC'GCC

Cuts at:      0       9    1389 
   Size:          9    1380
 
SmaI CCC'GGG

Cuts at:      0    1126    1389 
   Size:       1126     263
 
SmlI C'TyrA_G

Cuts at:      0    1264    1306    1389 
   Size:       1264      42      83

  Fragments arranged by size: 

                1264      83      42
 
StuI AGG'CCT

Cuts at:      0     323    1378    1389 
   Size:        323    1055      11

  Fragments arranged by size: 

                1055     323      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     762    1389 
   Size:        762     627
 
TatI w'GTAC_w

Cuts at:      0     701     752    1358    1389 
   Size:        701      51     606      31

  Fragments arranged by size: 

                 701     606      51      31
 
XmaI C'CCGG_G

Cuts at:      0    1124    1389 
   Size:       1124     265
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AgeI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BstXI    BstYI     BtgI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PmlI    PpuMI     PstI    PvuII
     SacI    SanDI     SapI     ScaI     SfcI     SfoI     SmaI     SmlI
     StuI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AhdI     AleI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BsaI    BsaBI    BsiEI    BsiWI     BsmI    BspEI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FalI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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