Restriction map for pF1KB7196
 (Linear) (Six-base) MAPSORT of: KIBB7196.seq  Check: 1343  from: 1  to: 1470



KIBB7196.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AclI AA'CG_TT

Cuts at:      0     555    1470 
   Size:        555     915
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1470 
   Size:        961     509
 
AflIII A'CryG_T

Cuts at:      0     984    1470 
   Size:        984     486
 
AleI CACnn'nnGTG

Cuts at:      0    1177    1470 
   Size:       1177     293
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     241    1470 
   Size:        241    1229
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1048    1470 
   Size:       1048     422
 
ApaLI G'TGCA_C

Cuts at:      0    1054    1470 
   Size:       1054     416
 
ApoI r'AATT_y

Cuts at:      0     396     841    1123    1198    1251    1470 
   Size:        396     445     282      75      53     219

  Fragments arranged by size: 

                 445     396     282     219      75      53
 
AvaI C'yCGr_G

Cuts at:      0     678     779    1470 
   Size:        678     101     691

  Fragments arranged by size: 

                 691     678     101
 
BamHI G'GATC_C

Cuts at:      0       3     635    1470 
   Size:          3     632     835

  Fragments arranged by size: 

                 835     632       3
 
BanII G_rGCy'C

Cuts at:      0      70    1009    1470 
   Size:         70     939     461

  Fragments arranged by size: 

                 939     461      70
 
BclI T'GATC_A

Cuts at:      0     803    1470 
   Size:        803     667
 
Bme1580I G_kGCm'C

Cuts at:      0    1058    1153    1470 
   Size:       1058      95     317

  Fragments arranged by size: 

                1058     317      95
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     103    1470 
   Size:        103    1367
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     100     941    1470 
   Size:        100     841     529

  Fragments arranged by size: 

                 841     529     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78    1470 
   Size:         78    1392
 
BsaBI GATnn'nnATC

Cuts at:      0     766    1470 
   Size:        766     704
 
BsaWI w'CCGG_w

Cuts at:      0    1001    1470 
   Size:       1001     469
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92     289     429     475    1470 
   Size:         92     197     140      46     995

  Fragments arranged by size: 

                 995     197     140      92      46
 
BseYI C'CCAG_C

Cuts at:      0     889    1470 
   Size:        889     581
 
BsiHKAI G_wGCw'C

Cuts at:      0      26      70     519    1058    1470 
   Size:         26      44     449     539     412

  Fragments arranged by size: 

                 539     449     412      44      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      26      70     519    1009    1058    1153    1470 
   Size:         26      44     449     490      49      95     317

  Fragments arranged by size: 

                 490     449     317      95      49      44      26
 
BspHI T'CATG_A

Cuts at:      0     383    1470 
   Size:        383    1087
 
BsrDI GCAATG_nn'

Cuts at:      0     351     531    1470 
   Size:        351     180     939

  Fragments arranged by size: 

                 939     351     180
 
BssSI C'ACGA_G

Cuts at:      0    1057    1470 
   Size:       1057     413
 
BstYI r'GATC_y

Cuts at:      0       3     365     635    1470 
   Size:          3     362     270     835

  Fragments arranged by size: 

                 835     362     270       3
 
BtsI GCAGTG_nn'

Cuts at:      0     866    1112    1470 
   Size:        866     246     358

  Fragments arranged by size: 

                 866     358     246
 
ClaI AT'CG_AT

Cuts at:      0     760    1470 
   Size:        760     710
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     648     683    1470 
   Size:        648      35     787

  Fragments arranged by size: 

                 787     648      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     490    1470 
   Size:        490     980
 
EarI CTCTTCn'nnn_

Cuts at:      0     282     406     968    1470 
   Size:        282     124     562     502

  Fragments arranged by size: 

                 562     502     282     124
 
EcoICRI GAG'CTC

Cuts at:      0      68    1470 
   Size:         68    1402
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     961    1470 
   Size:         78     883     509

  Fragments arranged by size: 

                 883     509      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     457    1470 
   Size:        457    1013
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1314    1395    1470 
   Size:       1314      81      75

  Fragments arranged by size: 

                1314      81      75
 
EcoRI G'AATT_C

Cuts at:      0     841    1470 
   Size:        841     629
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     317     349     911     943    1470 
   Size:        317      32     562      32     527

  Fragments arranged by size: 

                 562     527     317      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     223     236    1177    1470 
   Size:        223      13     941     293

  Fragments arranged by size: 

                 941     293     223      13
 
MspA1I CmG'CkG

Cuts at:      0     449     874    1452    1470 
   Size:        449     425     578      18

  Fragments arranged by size: 

                 578     449     425      18
 
NspI r_CATG'y

Cuts at:      0    1017    1035    1470 
   Size:       1017      18     435

  Fragments arranged by size: 

                1017     435      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     115    1470 
   Size:        115    1355
 
PfoI T'CCnGG_A

Cuts at:      0     968    1470 
   Size:        968     502
 
PpuMI rG'GwC_Cy

Cuts at:      0    1395    1470 
   Size:       1395      75
 
PshAI GACnn'nnGTC

Cuts at:      0    1226    1470 
   Size:       1226     244
 
PsiI TTA'TAA

Cuts at:      0     566    1470 
   Size:        566     904
 
PstI C_TGCA'G

Cuts at:      0     873    1470 
   Size:        873     597
 
PvuII CAG'CTG

Cuts at:      0     874    1452    1470 
   Size:        874     578      18

  Fragments arranged by size: 

                 874     578      18
 
SacI G_AGCT'C

Cuts at:      0      70    1470 
   Size:         70    1400
 
SfcI C'TryA_G

Cuts at:      0     127     869    1470 
   Size:        127     742     601

  Fragments arranged by size: 

                 742     601     127
 
SmaI CCC'GGG

Cuts at:      0     680    1470 
   Size:        680     790
 
SphI G_CATG'C

Cuts at:      0    1017    1470 
   Size:       1017     453
 
StyI C'CwwG_G

Cuts at:      0     476    1145    1470 
   Size:        476     669     325

  Fragments arranged by size: 

                 669     476     325
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     962    1470 
   Size:        962     508
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     227     259    1470 
   Size:        227      32    1211

  Fragments arranged by size: 

                1211     227      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504    1470 
   Size:        504     966
 
XmaI C'CCGG_G

Cuts at:      0     678    1470 
   Size:        678     792
 
XmnI GAAnn'nnTTC

Cuts at:      0     556    1470 
   Size:        556     914
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     ApoI
     AvaI    BamHI    BanII     BclI Bme1580I     BmrI     BplI     BpmI
    BsaBI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BssSI    BstYI     BtsI     ClaI    CspCI   DraIII     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I     MslI   MspA1I     NspI
    PflMI     PfoI    PpuMI    PshAI     PsiI     PstI    PvuII     SacI
     SfcI     SmaI     SphI     StyI    TaqII     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     DraI     DrdI     EaeI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     ZraI
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